Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in Sahel goat in Semi-Arid region
Abstract Background Despite the widespread use of dexamethasone in veterinary and human medicine, it is reported to cause some severe pregnancy related side effects like abortion in some animals. The mechanism of the response is not clear but seems to be related to interspecies and/or breed differen...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Society of Animal Sciences and Technology
2017-05-01
|
| Series: | Journal of Animal Science and Technology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40781-017-0137-5 |
| _version_ | 1811340691295436800 |
|---|---|
| author | Dauda Yahi Nicholas Adetayo Ojo Gideon Dauda Mshelia |
| author_facet | Dauda Yahi Nicholas Adetayo Ojo Gideon Dauda Mshelia |
| author_sort | Dauda Yahi |
| collection | DOAJ |
| description | Abstract Background Despite the widespread use of dexamethasone in veterinary and human medicine, it is reported to cause some severe pregnancy related side effects like abortion in some animals. The mechanism of the response is not clear but seems to be related to interspecies and/or breed difference in response which may involve alterations in the concentrations of some reproductive hormones. Methods Twenty Sahel goats comprising 18 does and 2 bucks were used for this study. Pregnancies were achieved by natural mating after synchronization. Repeated dexamethasone injections were given at 0.25 mg/kg body weight. Blood samples were collected biweekly for hormonal assay. Uterine biopsies were harvested at days 28 and day 78 of gestation through caesarean section for immunohistochemical analysis using 3 pregnant does randomly selected from each group at each instant. Data were expressed as Means ± Standard Deviations and analyzed using statistical soft ware package, GraphPad Instant, version 3.0 (2003) and progesterone receptor (PR) were scored semi-quantitatively. Results Dexamethasone treatments had no significant (p > 0.05) effect on progesterone and estrogen concentrations in pregnant Sahel goats but up regulated PR from 2+ to 3+ in second trimester. Conclusion As dexamethasone adverse effect on placenta is an established fact, the lack of effect on progesterone level in this study may be due to the fact that unlike other species whose progesterone production during pregnancy is placenta – dependent, in goats is corpus luteum - dependent. Consequently dexamethasone adverse effect on placenta reported in literatures did not influence progesterone levels during pregnancy in Sahel goat. The up regulation of progesterone receptor (PR) in Sahel goat gravid uterus is a beneficial effects and that dexamethasone can safely be used in corpus luteum – dependent progesterone secreting pregnant animal species like Sahel goat and camel. Therefore source of progesterone secretions during pregnancy should be considered in clinical application of dexamethasone in pregnancy. |
| first_indexed | 2024-04-13T18:45:39Z |
| format | Article |
| id | doaj.art-cca520639bf143b084a74c91c18b601b |
| institution | Directory Open Access Journal |
| issn | 2055-0391 |
| language | English |
| last_indexed | 2024-04-13T18:45:39Z |
| publishDate | 2017-05-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj.art-cca520639bf143b084a74c91c18b601b2022-12-22T02:34:36ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2055-03912017-05-015911710.1186/s40781-017-0137-5Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in Sahel goat in Semi-Arid regionDauda Yahi0Nicholas Adetayo Ojo1Gideon Dauda Mshelia2Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of MaiduguriDepartment of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of MaiduguriDepartment of Theriogenology, Faculty of Veterinary Medicine, University of MaiduguriAbstract Background Despite the widespread use of dexamethasone in veterinary and human medicine, it is reported to cause some severe pregnancy related side effects like abortion in some animals. The mechanism of the response is not clear but seems to be related to interspecies and/or breed difference in response which may involve alterations in the concentrations of some reproductive hormones. Methods Twenty Sahel goats comprising 18 does and 2 bucks were used for this study. Pregnancies were achieved by natural mating after synchronization. Repeated dexamethasone injections were given at 0.25 mg/kg body weight. Blood samples were collected biweekly for hormonal assay. Uterine biopsies were harvested at days 28 and day 78 of gestation through caesarean section for immunohistochemical analysis using 3 pregnant does randomly selected from each group at each instant. Data were expressed as Means ± Standard Deviations and analyzed using statistical soft ware package, GraphPad Instant, version 3.0 (2003) and progesterone receptor (PR) were scored semi-quantitatively. Results Dexamethasone treatments had no significant (p > 0.05) effect on progesterone and estrogen concentrations in pregnant Sahel goats but up regulated PR from 2+ to 3+ in second trimester. Conclusion As dexamethasone adverse effect on placenta is an established fact, the lack of effect on progesterone level in this study may be due to the fact that unlike other species whose progesterone production during pregnancy is placenta – dependent, in goats is corpus luteum - dependent. Consequently dexamethasone adverse effect on placenta reported in literatures did not influence progesterone levels during pregnancy in Sahel goat. The up regulation of progesterone receptor (PR) in Sahel goat gravid uterus is a beneficial effects and that dexamethasone can safely be used in corpus luteum – dependent progesterone secreting pregnant animal species like Sahel goat and camel. Therefore source of progesterone secretions during pregnancy should be considered in clinical application of dexamethasone in pregnancy.http://link.springer.com/article/10.1186/s40781-017-0137-5DexamethasoneEstrogenPregnancyProgesteroneSahel goatUterus |
| spellingShingle | Dauda Yahi Nicholas Adetayo Ojo Gideon Dauda Mshelia Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in Sahel goat in Semi-Arid region Journal of Animal Science and Technology Dexamethasone Estrogen Pregnancy Progesterone Sahel goat Uterus |
| title | Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in Sahel goat in Semi-Arid region |
| title_full | Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in Sahel goat in Semi-Arid region |
| title_fullStr | Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in Sahel goat in Semi-Arid region |
| title_full_unstemmed | Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in Sahel goat in Semi-Arid region |
| title_short | Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in Sahel goat in Semi-Arid region |
| title_sort | effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in sahel goat in semi arid region |
| topic | Dexamethasone Estrogen Pregnancy Progesterone Sahel goat Uterus |
| url | http://link.springer.com/article/10.1186/s40781-017-0137-5 |
| work_keys_str_mv | AT daudayahi effectsofdexamethasoneonprogesteroneandestrogenprofilesanduterineprogesteronereceptorlocalizationduringpregnancyinsahelgoatinsemiaridregion AT nicholasadetayoojo effectsofdexamethasoneonprogesteroneandestrogenprofilesanduterineprogesteronereceptorlocalizationduringpregnancyinsahelgoatinsemiaridregion AT gideondaudamshelia effectsofdexamethasoneonprogesteroneandestrogenprofilesanduterineprogesteronereceptorlocalizationduringpregnancyinsahelgoatinsemiaridregion |