RP5-1148A21.3 (lncRP5) exerts oncogenic function in human ovarian carcinoma
Ovarian cancer (OC) is a fatal gynecological malignancy that is difficult to diagnose at early stages. Various long non-coding RNAs (lncRNAs) are aberrantly expressed in OC and exert regulatory effects on OC; however, the underlying mechanism requires in-depth investigation. This work is designed to...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
China Science Publishing & Media Ltd.
2022-01-01
|
| Series: | Acta Biochimica et Biophysica Sinica |
| Subjects: | |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2022002 |
| _version_ | 1797635844983488512 |
|---|---|
| author | Sun Pingping Bao Aimei Hua Xiangdong Cao Jian Ding Ye |
| author_facet | Sun Pingping Bao Aimei Hua Xiangdong Cao Jian Ding Ye |
| author_sort | Sun Pingping |
| collection | DOAJ |
| description | Ovarian cancer (OC) is a fatal gynecological malignancy that is difficult to diagnose at early stages. Various long non-coding RNAs (lncRNAs) are aberrantly expressed in OC and exert regulatory effects on OC; however, the underlying mechanism requires in-depth investigation. This work is designed to explore the molecular regulatory axis of a newly identified lncRNA in OC, that is, lncRNA RP5-1148A21.3 (lncRP5). RT-qPCR shows lncRP5 is significantly upregulated in OC patients and cell lines, and it is mainly located in the cytoplasm of OC cells. The results of CCK-8, colony formation, and transwell assays demonstrate that overexpression of lncRP5 greatly contributes to malignant behaviors of OC cells, while inhibition of lncRP5 shows the opposite effects. Moreover, the binding relationship between lncRP5 and miR-545-5p is predicted by bioinformatics and is further verified by luciferase assay. Functionally, the regulatory effects of lncRP5 and miR-545-3p are negatively related; miR-545-5p serves as a tumor suppressor in OC. Further studies demonstrate that PTP4A1 is the target gene of miR-545-5p. Overexpression of PTP4A1 abrogates the inhibitory function of miR-545-5p on OC cell growth and metastasis. The lncRP5/miR-545-5p/PTP4A1 axis is subsequently demonstrated in vivo, and knockdown of lncRP5 notably inhibits tumor growth. This study provides a novel regulatory mechanism of OC, which may contribute to the diagnosis and therapy of OC. |
| first_indexed | 2024-03-11T12:26:06Z |
| format | Article |
| id | doaj.art-cca6bfc8082b4669a19e44c1fc82eaf0 |
| institution | Directory Open Access Journal |
| issn | 1672-9145 |
| language | English |
| last_indexed | 2024-03-11T12:26:06Z |
| publishDate | 2022-01-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj.art-cca6bfc8082b4669a19e44c1fc82eaf02023-11-06T09:02:46ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452022-01-015420921910.3724/abbs.202200220d259ccRP5-1148A21.3 (lncRP5) exerts oncogenic function in human ovarian carcinomaSun Pingping0Bao Aimei1Hua Xiangdong2Cao Jian3Ding Ye4[][][][][]Ovarian cancer (OC) is a fatal gynecological malignancy that is difficult to diagnose at early stages. Various long non-coding RNAs (lncRNAs) are aberrantly expressed in OC and exert regulatory effects on OC; however, the underlying mechanism requires in-depth investigation. This work is designed to explore the molecular regulatory axis of a newly identified lncRNA in OC, that is, lncRNA RP5-1148A21.3 (lncRP5). RT-qPCR shows lncRP5 is significantly upregulated in OC patients and cell lines, and it is mainly located in the cytoplasm of OC cells. The results of CCK-8, colony formation, and transwell assays demonstrate that overexpression of lncRP5 greatly contributes to malignant behaviors of OC cells, while inhibition of lncRP5 shows the opposite effects. Moreover, the binding relationship between lncRP5 and miR-545-5p is predicted by bioinformatics and is further verified by luciferase assay. Functionally, the regulatory effects of lncRP5 and miR-545-3p are negatively related; miR-545-5p serves as a tumor suppressor in OC. Further studies demonstrate that PTP4A1 is the target gene of miR-545-5p. Overexpression of PTP4A1 abrogates the inhibitory function of miR-545-5p on OC cell growth and metastasis. The lncRP5/miR-545-5p/PTP4A1 axis is subsequently demonstrated in vivo, and knockdown of lncRP5 notably inhibits tumor growth. This study provides a novel regulatory mechanism of OC, which may contribute to the diagnosis and therapy of OC.https://www.sciengine.com/doi/10.3724/abbs.2022002ovarian cancermiR-545-5pPTP4A1RP5-1148A21.3 (lncRP5) |
| spellingShingle | Sun Pingping Bao Aimei Hua Xiangdong Cao Jian Ding Ye RP5-1148A21.3 (lncRP5) exerts oncogenic function in human ovarian carcinoma Acta Biochimica et Biophysica Sinica ovarian cancer miR-545-5p PTP4A1 RP5-1148A21.3 (lncRP5) |
| title | RP5-1148A21.3 (lncRP5) exerts oncogenic function in human ovarian carcinoma |
| title_full | RP5-1148A21.3 (lncRP5) exerts oncogenic function in human ovarian carcinoma |
| title_fullStr | RP5-1148A21.3 (lncRP5) exerts oncogenic function in human ovarian carcinoma |
| title_full_unstemmed | RP5-1148A21.3 (lncRP5) exerts oncogenic function in human ovarian carcinoma |
| title_short | RP5-1148A21.3 (lncRP5) exerts oncogenic function in human ovarian carcinoma |
| title_sort | rp5 1148a21 3 lncrp5 exerts oncogenic function in human ovarian carcinoma |
| topic | ovarian cancer miR-545-5p PTP4A1 RP5-1148A21.3 (lncRP5) |
| url | https://www.sciengine.com/doi/10.3724/abbs.2022002 |
| work_keys_str_mv | AT sunpingping rp51148a213lncrp5exertsoncogenicfunctioninhumanovariancarcinoma AT baoaimei rp51148a213lncrp5exertsoncogenicfunctioninhumanovariancarcinoma AT huaxiangdong rp51148a213lncrp5exertsoncogenicfunctioninhumanovariancarcinoma AT caojian rp51148a213lncrp5exertsoncogenicfunctioninhumanovariancarcinoma AT dingye rp51148a213lncrp5exertsoncogenicfunctioninhumanovariancarcinoma |