Simultaneous production of detergent stable keratinolytic protease, amylase and biosurfactant by Bacillus subtilis PF1 using agro industrial waste
The present study is an attempt to optimize simultaneous production of keratinolytic protease, amylase and biosurfactant from feather meal, potato peel and rape seed cake in a single media by response surface methodology to evaluate their biochemical properties for detergent additive. The optimizati...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-06-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X16300145 |
| _version_ | 1818672439810326528 |
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| author | Khushboo Bhange Venkatesh Chaturvedi Renu Bhatt |
| author_facet | Khushboo Bhange Venkatesh Chaturvedi Renu Bhatt |
| author_sort | Khushboo Bhange |
| collection | DOAJ |
| description | The present study is an attempt to optimize simultaneous production of keratinolytic protease, amylase and biosurfactant from feather meal, potato peel and rape seed cake in a single media by response surface methodology to evaluate their biochemical properties for detergent additive. The optimization was carried out using 20 run, 3 factor and 5-level of central composite design on design expert software which resulted in a 1.2, 0.84 and 2.28 fold increase in protease, amylase and biosurfactant production. The proteolytic activity was found to be optimum at pH 9.0 and 60 °C while optimum amylolytic activity was recorded at pH 6.0 and 70 °C respectively. Both enzymes were found to be stable in the presence of organic solvents, ionic and commercial detergent and oxidizing agents. The biosurfactant was extracted with chloroform and was found to be stable at varying pH and temperature; however a reduction in the activity was observed at temperature higher than 70 °C. The isolated enzymes and biosurfactants may find applications in the effective removal of stains. |
| first_indexed | 2024-12-17T07:39:55Z |
| format | Article |
| id | doaj.art-cca94082d7b24decbf0860a821689c2a |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-17T07:39:55Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-cca94082d7b24decbf0860a821689c2a2022-12-21T21:58:11ZengElsevierBiotechnology Reports2215-017X2016-06-0110C9410410.1016/j.btre.2016.03.007Simultaneous production of detergent stable keratinolytic protease, amylase and biosurfactant by Bacillus subtilis PF1 using agro industrial wasteKhushboo Bhange0Venkatesh Chaturvedi1Renu Bhatt2Department of Biotechnology, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur 495009, Chhattisgarh, IndiaSchool of Biotechnology, Banaras Hindu University, Varanasi, U.P., IndiaDepartment of Biotechnology, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur 495009, Chhattisgarh, IndiaThe present study is an attempt to optimize simultaneous production of keratinolytic protease, amylase and biosurfactant from feather meal, potato peel and rape seed cake in a single media by response surface methodology to evaluate their biochemical properties for detergent additive. The optimization was carried out using 20 run, 3 factor and 5-level of central composite design on design expert software which resulted in a 1.2, 0.84 and 2.28 fold increase in protease, amylase and biosurfactant production. The proteolytic activity was found to be optimum at pH 9.0 and 60 °C while optimum amylolytic activity was recorded at pH 6.0 and 70 °C respectively. Both enzymes were found to be stable in the presence of organic solvents, ionic and commercial detergent and oxidizing agents. The biosurfactant was extracted with chloroform and was found to be stable at varying pH and temperature; however a reduction in the activity was observed at temperature higher than 70 °C. The isolated enzymes and biosurfactants may find applications in the effective removal of stains.http://www.sciencedirect.com/science/article/pii/S2215017X16300145ProteaseAmylaseBiosurfactantBacillus subtilis PF1 |
| spellingShingle | Khushboo Bhange Venkatesh Chaturvedi Renu Bhatt Simultaneous production of detergent stable keratinolytic protease, amylase and biosurfactant by Bacillus subtilis PF1 using agro industrial waste Biotechnology Reports Protease Amylase Biosurfactant Bacillus subtilis PF1 |
| title | Simultaneous production of detergent stable keratinolytic protease, amylase and biosurfactant by Bacillus subtilis PF1 using agro industrial waste |
| title_full | Simultaneous production of detergent stable keratinolytic protease, amylase and biosurfactant by Bacillus subtilis PF1 using agro industrial waste |
| title_fullStr | Simultaneous production of detergent stable keratinolytic protease, amylase and biosurfactant by Bacillus subtilis PF1 using agro industrial waste |
| title_full_unstemmed | Simultaneous production of detergent stable keratinolytic protease, amylase and biosurfactant by Bacillus subtilis PF1 using agro industrial waste |
| title_short | Simultaneous production of detergent stable keratinolytic protease, amylase and biosurfactant by Bacillus subtilis PF1 using agro industrial waste |
| title_sort | simultaneous production of detergent stable keratinolytic protease amylase and biosurfactant by bacillus subtilis pf1 using agro industrial waste |
| topic | Protease Amylase Biosurfactant Bacillus subtilis PF1 |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X16300145 |
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