Efficacy of Lamium album as a natural fungicide: impact on seed germination, ergosterol, and mycotoxins in Fusarium culmorum-infected wheat seedlings
Fusarium culmorum is a major wheat pathogen, and its secondary metabolites (mycotoxins) cause damage to plants, animals, and human health. In the era of sustainable agriculture, eco-friendly methods of prevention and control are constantly needed. The use of plant extracts as biocontrol agents has g...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1363204/full |
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| author | Pascaline Aimee Uwineza Monika Urbaniak Łukasz Stępień Anna Gramza-Michałowska Agnieszka Waśkiewicz |
| author_facet | Pascaline Aimee Uwineza Monika Urbaniak Łukasz Stępień Anna Gramza-Michałowska Agnieszka Waśkiewicz |
| author_sort | Pascaline Aimee Uwineza |
| collection | DOAJ |
| description | Fusarium culmorum is a major wheat pathogen, and its secondary metabolites (mycotoxins) cause damage to plants, animals, and human health. In the era of sustainable agriculture, eco-friendly methods of prevention and control are constantly needed. The use of plant extracts as biocontrol agents has gained popularity as they are a source of active substances that play a crucial role in fighting against phytopathogens. This study evaluated the impact of Lamium album on wheat seed germination and seedling growth. In a pot experiment, the effect of L. album on wheat seedlings artificially inoculated with F. culmorum was evaluated by measuring seedling growth parameters, and by using chromatographic methods, ergosterol and mycotoxins levels were analyzed. The results showed that the phytotoxic effect of L. album flower extracts on wheat seed germination and seedling growth was concentration dependent. The radicle length was also reduced compared to the control; however, L. album did not significantly affect the dry weight of the radicle. A slight phytotoxic effect on seed germination was observed, but antifungal effects on artificially infected wheat seedlings were also confirmed with the reduction of ergosterol level and mycotoxins accumulation in the roots and leaves after 21 days of inoculation. F. culmorum DNA was identified in the control samples only. Overall, this study is a successful in planta study showing L. album flower extract protection of wheat against the pathogen responsible for Fusarium crown and root rot. Further research is essential to study the effects of L. album extracts on key regulatory genes for mycotoxin biosynthetic pathways. |
| first_indexed | 2024-03-07T22:55:40Z |
| format | Article |
| id | doaj.art-cca9808f0c7047d8adec38dc7d70fb3b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-07T22:55:40Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cca9808f0c7047d8adec38dc7d70fb3b2024-02-23T04:51:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-02-011510.3389/fmicb.2024.13632041363204Efficacy of Lamium album as a natural fungicide: impact on seed germination, ergosterol, and mycotoxins in Fusarium culmorum-infected wheat seedlingsPascaline Aimee Uwineza0Monika Urbaniak1Łukasz Stępień2Anna Gramza-Michałowska3Agnieszka Waśkiewicz4Department of Chemistry, Poznan University of Life Sciences, Poznan, PolandPlant-Pathogen Interaction Team, Institute of Plant Genetics, Polish Academy of Sciences, Poznan, PolandPlant-Pathogen Interaction Team, Institute of Plant Genetics, Polish Academy of Sciences, Poznan, PolandDepartment of Gastronomy Science and Functional Foods, Poznan University of Life Sciences, Poznan, PolandDepartment of Chemistry, Poznan University of Life Sciences, Poznan, PolandFusarium culmorum is a major wheat pathogen, and its secondary metabolites (mycotoxins) cause damage to plants, animals, and human health. In the era of sustainable agriculture, eco-friendly methods of prevention and control are constantly needed. The use of plant extracts as biocontrol agents has gained popularity as they are a source of active substances that play a crucial role in fighting against phytopathogens. This study evaluated the impact of Lamium album on wheat seed germination and seedling growth. In a pot experiment, the effect of L. album on wheat seedlings artificially inoculated with F. culmorum was evaluated by measuring seedling growth parameters, and by using chromatographic methods, ergosterol and mycotoxins levels were analyzed. The results showed that the phytotoxic effect of L. album flower extracts on wheat seed germination and seedling growth was concentration dependent. The radicle length was also reduced compared to the control; however, L. album did not significantly affect the dry weight of the radicle. A slight phytotoxic effect on seed germination was observed, but antifungal effects on artificially infected wheat seedlings were also confirmed with the reduction of ergosterol level and mycotoxins accumulation in the roots and leaves after 21 days of inoculation. F. culmorum DNA was identified in the control samples only. Overall, this study is a successful in planta study showing L. album flower extract protection of wheat against the pathogen responsible for Fusarium crown and root rot. Further research is essential to study the effects of L. album extracts on key regulatory genes for mycotoxin biosynthetic pathways.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1363204/fullTriticum genusplant extractLamiaceaePCRtrichotheceneszearalenone |
| spellingShingle | Pascaline Aimee Uwineza Monika Urbaniak Łukasz Stępień Anna Gramza-Michałowska Agnieszka Waśkiewicz Efficacy of Lamium album as a natural fungicide: impact on seed germination, ergosterol, and mycotoxins in Fusarium culmorum-infected wheat seedlings Frontiers in Microbiology Triticum genus plant extract Lamiaceae PCR trichothecenes zearalenone |
| title | Efficacy of Lamium album as a natural fungicide: impact on seed germination, ergosterol, and mycotoxins in Fusarium culmorum-infected wheat seedlings |
| title_full | Efficacy of Lamium album as a natural fungicide: impact on seed germination, ergosterol, and mycotoxins in Fusarium culmorum-infected wheat seedlings |
| title_fullStr | Efficacy of Lamium album as a natural fungicide: impact on seed germination, ergosterol, and mycotoxins in Fusarium culmorum-infected wheat seedlings |
| title_full_unstemmed | Efficacy of Lamium album as a natural fungicide: impact on seed germination, ergosterol, and mycotoxins in Fusarium culmorum-infected wheat seedlings |
| title_short | Efficacy of Lamium album as a natural fungicide: impact on seed germination, ergosterol, and mycotoxins in Fusarium culmorum-infected wheat seedlings |
| title_sort | efficacy of lamium album as a natural fungicide impact on seed germination ergosterol and mycotoxins in fusarium culmorum infected wheat seedlings |
| topic | Triticum genus plant extract Lamiaceae PCR trichothecenes zearalenone |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1363204/full |
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