New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species <i>Sanghuangporus</i><i>baumii</i> (Pilát) L.W. Zhou & Y.C. Dai
Triterpenoids are secondary metabolites produced by the fungus <i>Sanghuangporus</i> <i>baumii</i> that have important pharmacological activities. However, the yield of triterpenoids is low and cannot meet market demand. Here, we treated <i>S. baumii</i> with seve...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/9/889 |
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| author | Zengcai Liu Ruipeng Liu Xinyu Tong Li Zou |
| author_facet | Zengcai Liu Ruipeng Liu Xinyu Tong Li Zou |
| author_sort | Zengcai Liu |
| collection | DOAJ |
| description | Triterpenoids are secondary metabolites produced by the fungus <i>Sanghuangporus</i> <i>baumii</i> that have important pharmacological activities. However, the yield of triterpenoids is low and cannot meet market demand. Here, we treated <i>S. baumii</i> with several concentrations of MeJA (methyl jasmonate) and found that the total triterpenoid content was highest (23.31 mg/g) when the MeJA concentration was 250 μmol/L. qRT-PCR was used to quantify the transcription of five key genes involved in triterpenoid biosynthesis. The results showed that the relative transcription of most genes increased with increasing MeJA concentration, indicating that MeJA is a potent inducer of triterpenoid biosynthesis in <i>S. baumii</i>. To further explore whether other terpenoid biosynthesis pathways are also involved in the accumulation of triterpenoids induced by MeJA, we measured the contents of cis-Zeatin (cZ), gibberellins (GAs), and the transcript levels of related biosynthesis genes. We found that MeJA significantly inhibited the biosynthesis of cZ, GAs, and the transcription of related genes. The repressive effects of MeJA on cZ and GA accumulation were further confirmed by growth rate and biomass assays. In conclusion, our study provides an effective method to enhance the triterpenoid content of <i>S. baumii</i>, and also provides novel insights into the mechanism of MeJA-induced triterpenoid biosynthesis. |
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| id | doaj.art-ccaa270a35364fcfa557d09f6c8a83ee |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T23:31:32Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-ccaa270a35364fcfa557d09f6c8a83ee2023-11-23T17:08:45ZengMDPI AGJournal of Fungi2309-608X2022-08-018988910.3390/jof8090889New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species <i>Sanghuangporus</i><i>baumii</i> (Pilát) L.W. Zhou & Y.C. DaiZengcai Liu0Ruipeng Liu1Xinyu Tong2Li Zou3College of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaTriterpenoids are secondary metabolites produced by the fungus <i>Sanghuangporus</i> <i>baumii</i> that have important pharmacological activities. However, the yield of triterpenoids is low and cannot meet market demand. Here, we treated <i>S. baumii</i> with several concentrations of MeJA (methyl jasmonate) and found that the total triterpenoid content was highest (23.31 mg/g) when the MeJA concentration was 250 μmol/L. qRT-PCR was used to quantify the transcription of five key genes involved in triterpenoid biosynthesis. The results showed that the relative transcription of most genes increased with increasing MeJA concentration, indicating that MeJA is a potent inducer of triterpenoid biosynthesis in <i>S. baumii</i>. To further explore whether other terpenoid biosynthesis pathways are also involved in the accumulation of triterpenoids induced by MeJA, we measured the contents of cis-Zeatin (cZ), gibberellins (GAs), and the transcript levels of related biosynthesis genes. We found that MeJA significantly inhibited the biosynthesis of cZ, GAs, and the transcription of related genes. The repressive effects of MeJA on cZ and GA accumulation were further confirmed by growth rate and biomass assays. In conclusion, our study provides an effective method to enhance the triterpenoid content of <i>S. baumii</i>, and also provides novel insights into the mechanism of MeJA-induced triterpenoid biosynthesis.https://www.mdpi.com/2309-608X/8/9/889<i>Sanghuangporus</i> <i>baumii</i>methyl jasmonateterpenoidstriterpenoid biosynthesisgrowth and development |
| spellingShingle | Zengcai Liu Ruipeng Liu Xinyu Tong Li Zou New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species <i>Sanghuangporus</i><i>baumii</i> (Pilát) L.W. Zhou & Y.C. Dai Journal of Fungi <i>Sanghuangporus</i> <i>baumii</i> methyl jasmonate terpenoids triterpenoid biosynthesis growth and development |
| title | New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species <i>Sanghuangporus</i><i>baumii</i> (Pilát) L.W. Zhou & Y.C. Dai |
| title_full | New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species <i>Sanghuangporus</i><i>baumii</i> (Pilát) L.W. Zhou & Y.C. Dai |
| title_fullStr | New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species <i>Sanghuangporus</i><i>baumii</i> (Pilát) L.W. Zhou & Y.C. Dai |
| title_full_unstemmed | New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species <i>Sanghuangporus</i><i>baumii</i> (Pilát) L.W. Zhou & Y.C. Dai |
| title_short | New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species <i>Sanghuangporus</i><i>baumii</i> (Pilát) L.W. Zhou & Y.C. Dai |
| title_sort | new insights into methyl jasmonate regulation of triterpenoid biosynthesis in medicinal fungal species i sanghuangporus i i baumii i pilat l w zhou y c dai |
| topic | <i>Sanghuangporus</i> <i>baumii</i> methyl jasmonate terpenoids triterpenoid biosynthesis growth and development |
| url | https://www.mdpi.com/2309-608X/8/9/889 |
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