| Summary: | Carbon dioxide (CO<sub>2</sub>) enrichment in an agricultural environment has been shown to enhance the efficiency of crop photosynthesis, increasing crop yield and product quality. There is a problem of the excessive use of CO<sub>2</sub> gas when the CO<sub>2</sub> is enriched for crops, such as soybean and other field crops. Given the application of micro-nanobubbles (MNBs) in agricultural production, this research takes CO<sub>2</sub> as the gas source to prepare the micro-nanobubble water by the dissolved gas release method, and the response surface methodology is used to optimize the preparation process. The results show that the optimum parameters, which are the gas–liquid ratio, generator running time, and inlet water temperature for the preparation of CO<sub>2</sub> micro-nanobubble water, are 2.87%, 28.47 min, and 25.52 °C, respectively. The CO<sub>2</sub> content in the MNB water prepared under the optimum parameters is 7.64 mg/L, and the pH is 4.08. Furthermore, the particle size of the bubbles is mostly 255.5 nm. With the extension of the storage time, some of the bubbles polymerize and spill out, but there is still a certain amount of nanoscale bubbles during a certain period. This research provides a new idea for using MNB technology to increase the content and lifespan of CO<sub>2</sub> in water, which will slow the release and increase the utilization of CO<sub>2</sub> when using CO<sub>2</sub> enrichment in agriculture.
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