Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study
AbstractObjectives The objective of this study was to investigate the glycolytic activity of adenomyosis, which is characterized by malignant biological behaviors including abnormal cell proliferation, migration, invasion, cell regulation, and epithelial-mesenchymal transition.Methods From January 2...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Gynecological Endocrinology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09513590.2024.2332411 |
| _version_ | 1797238781924868096 |
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| author | Shiya Huang Xueqian Huang Xiaohui Wen Xuehong Liu Hongxia Ma Linling Xie Yishu Wang Shanjia Liu Yongge Guan Kunyin Li |
| author_facet | Shiya Huang Xueqian Huang Xiaohui Wen Xuehong Liu Hongxia Ma Linling Xie Yishu Wang Shanjia Liu Yongge Guan Kunyin Li |
| author_sort | Shiya Huang |
| collection | DOAJ |
| description | AbstractObjectives The objective of this study was to investigate the glycolytic activity of adenomyosis, which is characterized by malignant biological behaviors including abnormal cell proliferation, migration, invasion, cell regulation, and epithelial-mesenchymal transition.Methods From January 2021 to August 2022, a total of 15 patients who underwent total hysterectomy for adenomyosis and 14 patients who had non-endometrial diseases, specifically with cervical squamous intraepithelial neoplasia and uterine myoma, were included in this study. Myometrium with ectopic endometrium from patients with adenomyosis while normal myometrium from patients in the control group were collected. All samples were confirmed by a histopathological examination. The samples were analyzed by liquid chromatography-mass spectrometry (LC-MS), real-time quantitative PCR, NAD+/NADH assay kit as well as the glucose and lactate assay kits.Results Endometrial stroma and glands could be observed within the myometrium of patients in the adenomyosis group. We found that the mRNA expressions of HK1, PFKFB3, glyceraldehyde-3-phospate dehydrogenase (GAPDH), PKM2, and PDHA as well as the protein expressions of PFKFB3 were elevated in ectopic endometrial tissues of the adenomyosis group as compared to normal myometrium of the control group. The level of fructose 1,6-diphosphate was increased while NAD + and NAD+/NADH ratio were decreased compared with the control group. Besides, increased glucose consumption and lactate production were observed in myometrium with ectopic endometrium.Conclusions We concluded that altered glycolytic phenotype of the myometrium with ectopic endometrium in women with adenomyosis may contribute the development of adenomyosis. |
| first_indexed | 2024-04-24T17:41:06Z |
| format | Article |
| id | doaj.art-ccad501a9f5948c98c8497da7134fc9a |
| institution | Directory Open Access Journal |
| issn | 0951-3590 1473-0766 |
| language | English |
| last_indexed | 2024-04-24T17:41:06Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gynecological Endocrinology |
| spelling | doaj.art-ccad501a9f5948c98c8497da7134fc9a2024-03-28T00:00:13ZengTaylor & Francis GroupGynecological Endocrinology0951-35901473-07662024-12-0140110.1080/09513590.2024.2332411Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary studyShiya Huang0Xueqian Huang1Xiaohui Wen2Xuehong Liu3Hongxia Ma4Linling Xie5Yishu Wang6Shanjia Liu7Yongge Guan8Kunyin Li9The Third Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaThe Third Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaThe Third Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaThe Third Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaDepartment of Traditional Chinese Medicine, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, PR ChinaThe Third Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaThe Third Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaThe Third Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaThe Third Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaThe Third Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaAbstractObjectives The objective of this study was to investigate the glycolytic activity of adenomyosis, which is characterized by malignant biological behaviors including abnormal cell proliferation, migration, invasion, cell regulation, and epithelial-mesenchymal transition.Methods From January 2021 to August 2022, a total of 15 patients who underwent total hysterectomy for adenomyosis and 14 patients who had non-endometrial diseases, specifically with cervical squamous intraepithelial neoplasia and uterine myoma, were included in this study. Myometrium with ectopic endometrium from patients with adenomyosis while normal myometrium from patients in the control group were collected. All samples were confirmed by a histopathological examination. The samples were analyzed by liquid chromatography-mass spectrometry (LC-MS), real-time quantitative PCR, NAD+/NADH assay kit as well as the glucose and lactate assay kits.Results Endometrial stroma and glands could be observed within the myometrium of patients in the adenomyosis group. We found that the mRNA expressions of HK1, PFKFB3, glyceraldehyde-3-phospate dehydrogenase (GAPDH), PKM2, and PDHA as well as the protein expressions of PFKFB3 were elevated in ectopic endometrial tissues of the adenomyosis group as compared to normal myometrium of the control group. The level of fructose 1,6-diphosphate was increased while NAD + and NAD+/NADH ratio were decreased compared with the control group. Besides, increased glucose consumption and lactate production were observed in myometrium with ectopic endometrium.Conclusions We concluded that altered glycolytic phenotype of the myometrium with ectopic endometrium in women with adenomyosis may contribute the development of adenomyosis.https://www.tandfonline.com/doi/10.1080/09513590.2024.2332411Adenomyosismyometrium with ectopic endometriumglycolysisglycolytic enzymeslactate |
| spellingShingle | Shiya Huang Xueqian Huang Xiaohui Wen Xuehong Liu Hongxia Ma Linling Xie Yishu Wang Shanjia Liu Yongge Guan Kunyin Li Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study Gynecological Endocrinology Adenomyosis myometrium with ectopic endometrium glycolysis glycolytic enzymes lactate |
| title | Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study |
| title_full | Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study |
| title_fullStr | Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study |
| title_full_unstemmed | Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study |
| title_short | Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study |
| title_sort | enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis a preliminary study |
| topic | Adenomyosis myometrium with ectopic endometrium glycolysis glycolytic enzymes lactate |
| url | https://www.tandfonline.com/doi/10.1080/09513590.2024.2332411 |
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