Use of Nutshells Wastes in the Production of Lignocellulolytic Enzymes by White-Rot Fungi
Abstract Agricultural wastes are an environmental and economic problem that countries must strive to solve as soon as possible. White-rot fungi can take advantage of these wastes through an efficient process involving the bioconversion of lignocellulosic materials. Therefore, this work has used pine...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2023-01-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132023000100203&tlng=en |
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| author | Adriana Ibarra-Islas Jazmín Edith Méndez Hernández Silvia Armenta Josefa Espitia López Paul Misael Garza López Sergio Hernández León Oscar Arce-Cervantes |
| author_facet | Adriana Ibarra-Islas Jazmín Edith Méndez Hernández Silvia Armenta Josefa Espitia López Paul Misael Garza López Sergio Hernández León Oscar Arce-Cervantes |
| author_sort | Adriana Ibarra-Islas |
| collection | DOAJ |
| description | Abstract Agricultural wastes are an environmental and economic problem that countries must strive to solve as soon as possible. White-rot fungi can take advantage of these wastes through an efficient process involving the bioconversion of lignocellulosic materials. Therefore, this work has used pine needles (Pinus pseudostrobus) and nutshells (Carya illinoinensis), relevant wastes in Mexico, as substrates for growing three fungal strains (Ganoderma lucidum, Lentinula edodes, and Pleurotus ostreatus) on PDA plates supplemented with these materials. Besides, the capacity to produce lignocellulolytic enzymes by these three fungi through solid-state fermentation has been evaluated using pine needles and nutshells along with barley straw. The relevant results suggest that nutshells are a potential substrate for growing G. lucidum (mycelial growth rate of 0.758 cm d-1). Albeit pine needles waste has allowed G. lucidum growth (0.402 cm d-1), L. edodes and P. ostreatus growth on PDA supplemented plates were not observed. Further to this, nutshells or pine needles combined with barley straw were used as substrates through solid-state fermentation to produce lignocellulolytic enzymes (cellulase, xylanase, and laccase). G. lucidum presented the highest cellulase productivity (3.03 ± 0.34 IU/gdm·d) using nutshells and barley straw (BS_NS) as substrates, while it was half using pine needles and barley straw (BS_PN) (1.35 ± 0.20 IU/gdm·d). The results were similar for xylanase productivity because G. lucidum produced 4.80 ± 0.61 IU/gdm·d using BS_PN, slightly higher than the BS_NS (3.79 ± 0.29 IU/gdm·). Finally, nutshells might be a suitable substrate for the growth of white-rot fungi, mainly the G. lucidum strain, making it an ecological alternative for future biotechnological applications. |
| first_indexed | 2024-04-10T19:03:49Z |
| format | Article |
| id | doaj.art-ccaeafe037894e68b578e48b3688b18e |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-10T19:03:49Z |
| publishDate | 2023-01-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-ccaeafe037894e68b578e48b3688b18e2023-01-31T07:33:02ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242023-01-016610.1590/1678-4324-2023210654Use of Nutshells Wastes in the Production of Lignocellulolytic Enzymes by White-Rot FungiAdriana Ibarra-Islashttps://orcid.org/0000-0002-8729-3015Jazmín Edith Méndez Hernándezhttps://orcid.org/0000-0002-6436-2882Silvia Armentahttps://orcid.org/0000-0001-8464-5591Josefa Espitia Lópezhttps://orcid.org/0000-0002-0026-7624Paul Misael Garza Lópezhttps://orcid.org/0000-0002-3151-8369Sergio Hernández Leónhttps://orcid.org/0000-0001-5892-6066Oscar Arce-Cervanteshttps://orcid.org/0000-0002-3388-2973Abstract Agricultural wastes are an environmental and economic problem that countries must strive to solve as soon as possible. White-rot fungi can take advantage of these wastes through an efficient process involving the bioconversion of lignocellulosic materials. Therefore, this work has used pine needles (Pinus pseudostrobus) and nutshells (Carya illinoinensis), relevant wastes in Mexico, as substrates for growing three fungal strains (Ganoderma lucidum, Lentinula edodes, and Pleurotus ostreatus) on PDA plates supplemented with these materials. Besides, the capacity to produce lignocellulolytic enzymes by these three fungi through solid-state fermentation has been evaluated using pine needles and nutshells along with barley straw. The relevant results suggest that nutshells are a potential substrate for growing G. lucidum (mycelial growth rate of 0.758 cm d-1). Albeit pine needles waste has allowed G. lucidum growth (0.402 cm d-1), L. edodes and P. ostreatus growth on PDA supplemented plates were not observed. Further to this, nutshells or pine needles combined with barley straw were used as substrates through solid-state fermentation to produce lignocellulolytic enzymes (cellulase, xylanase, and laccase). G. lucidum presented the highest cellulase productivity (3.03 ± 0.34 IU/gdm·d) using nutshells and barley straw (BS_NS) as substrates, while it was half using pine needles and barley straw (BS_PN) (1.35 ± 0.20 IU/gdm·d). The results were similar for xylanase productivity because G. lucidum produced 4.80 ± 0.61 IU/gdm·d using BS_PN, slightly higher than the BS_NS (3.79 ± 0.29 IU/gdm·). Finally, nutshells might be a suitable substrate for the growth of white-rot fungi, mainly the G. lucidum strain, making it an ecological alternative for future biotechnological applications.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132023000100203&tlng=enLignocellulosic enzymesGanoderma lucidumLentinula edodesPleurotus ostreatusnutshells wastepine needles waste. |
| spellingShingle | Adriana Ibarra-Islas Jazmín Edith Méndez Hernández Silvia Armenta Josefa Espitia López Paul Misael Garza López Sergio Hernández León Oscar Arce-Cervantes Use of Nutshells Wastes in the Production of Lignocellulolytic Enzymes by White-Rot Fungi Brazilian Archives of Biology and Technology Lignocellulosic enzymes Ganoderma lucidum Lentinula edodes Pleurotus ostreatus nutshells waste pine needles waste. |
| title | Use of Nutshells Wastes in the Production of Lignocellulolytic Enzymes by White-Rot Fungi |
| title_full | Use of Nutshells Wastes in the Production of Lignocellulolytic Enzymes by White-Rot Fungi |
| title_fullStr | Use of Nutshells Wastes in the Production of Lignocellulolytic Enzymes by White-Rot Fungi |
| title_full_unstemmed | Use of Nutshells Wastes in the Production of Lignocellulolytic Enzymes by White-Rot Fungi |
| title_short | Use of Nutshells Wastes in the Production of Lignocellulolytic Enzymes by White-Rot Fungi |
| title_sort | use of nutshells wastes in the production of lignocellulolytic enzymes by white rot fungi |
| topic | Lignocellulosic enzymes Ganoderma lucidum Lentinula edodes Pleurotus ostreatus nutshells waste pine needles waste. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132023000100203&tlng=en |
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