Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper Virus
Canine distemper (CD) caused by canine distemper virus (CDV) is one of the major infectious diseases in minks, bringing serious economic losses to the mink breeding industry. By an integrated analysis of microRNA (miRNA)-messenger RNA (mRNA), the present study analyzed the changes in the mink transc...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.897740/full |
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| author | Qiang Chen Qiang Chen Qiang Chen Mingwei Tong Na Sun Yong Yang Yuening Cheng Li Yi Gaili Wang Zhigang Cao Quan Zhao Shipeng Cheng |
| author_facet | Qiang Chen Qiang Chen Qiang Chen Mingwei Tong Na Sun Yong Yang Yuening Cheng Li Yi Gaili Wang Zhigang Cao Quan Zhao Shipeng Cheng |
| author_sort | Qiang Chen |
| collection | DOAJ |
| description | Canine distemper (CD) caused by canine distemper virus (CDV) is one of the major infectious diseases in minks, bringing serious economic losses to the mink breeding industry. By an integrated analysis of microRNA (miRNA)-messenger RNA (mRNA), the present study analyzed the changes in the mink transcriptome upon CDV infection in mink lung epithelial cells (Mv. l. Lu cells) for the first time. A total of 4,734 differentially expressed mRNAs (2,691 upregulated and 2,043 downregulated) with |log2(FoldChange) |>1 and P-adj<0.05 and 181 differentially expressed miRNAs (152 upregulated and 29 downregulated) with |log2(FoldChange) |>2 and P-adj<0.05 were identified. Gene Ontology (GO) enrichment indicated that differentially expressed genes (DEGs) were associated with various biological processes and molecular function, such as response to stimulus, cell communication, signaling, cytokine activity, transmembrane signaling receptor activity and signaling receptor activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the combination of miRNA and mRNA was done for immune and inflammatory responses, such as Janus kinase (JAK)-signal transducer and activator (STAT) signaling pathway and nuclear factor (NF)-kappa B signaling pathway. The enrichment analysis of target mRNA of differentially expressed miRNA revealed that mir-140-5p and mir-378-12 targeted corresponding genes to regulate NF-kappa B signaling pathway. JAK-STAT signaling pathway could be modulated by mir-425-2, mir-139-4, mir-140-6, mir-145-3, mir-140-5p and mir-204-2. This study compared the influence of miRNA-mRNA expression in Mv. l. Lu cells before and after CDV infection by integrated analysis of miRNA-mRNA and analyzed the complex network interaction between virus and host cells. The results can help understand the molecular mechanism of the natural immune response induced by CDV infection in host cells. |
| first_indexed | 2024-04-12T17:01:46Z |
| format | Article |
| id | doaj.art-ccb2a1b409014309917b9e9cc3354802 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-12T17:01:46Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-ccb2a1b409014309917b9e9cc33548022022-12-22T03:24:03ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-05-01910.3389/fvets.2022.897740897740Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper VirusQiang Chen0Qiang Chen1Qiang Chen2Mingwei Tong3Na Sun4Yong Yang5Yuening Cheng6Li Yi7Gaili Wang8Zhigang Cao9Quan Zhao10Shipeng Cheng11College of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Special Animal Epidemic Disease, Ministry of Agriculture, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaCollege of Landscape Architecture, Changchun University, Changchun, ChinaSchool of Basic Medical Sciences, Shanxi Medical University, Taiyuan, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin City, ChinaSchool of Basic Medical Sciences, Shanxi Medical University, Taiyuan, ChinaKey Laboratory of Special Animal Epidemic Disease, Ministry of Agriculture, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaKey Laboratory of Special Animal Epidemic Disease, Ministry of Agriculture, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaJilin Academy of Animal Husbandry and Veterinary Medicine, Changchun, ChinaKey Laboratory of Special Animal Epidemic Disease, Ministry of Agriculture, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaCollege of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Special Animal Epidemic Disease, Ministry of Agriculture, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaCanine distemper (CD) caused by canine distemper virus (CDV) is one of the major infectious diseases in minks, bringing serious economic losses to the mink breeding industry. By an integrated analysis of microRNA (miRNA)-messenger RNA (mRNA), the present study analyzed the changes in the mink transcriptome upon CDV infection in mink lung epithelial cells (Mv. l. Lu cells) for the first time. A total of 4,734 differentially expressed mRNAs (2,691 upregulated and 2,043 downregulated) with |log2(FoldChange) |>1 and P-adj<0.05 and 181 differentially expressed miRNAs (152 upregulated and 29 downregulated) with |log2(FoldChange) |>2 and P-adj<0.05 were identified. Gene Ontology (GO) enrichment indicated that differentially expressed genes (DEGs) were associated with various biological processes and molecular function, such as response to stimulus, cell communication, signaling, cytokine activity, transmembrane signaling receptor activity and signaling receptor activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the combination of miRNA and mRNA was done for immune and inflammatory responses, such as Janus kinase (JAK)-signal transducer and activator (STAT) signaling pathway and nuclear factor (NF)-kappa B signaling pathway. The enrichment analysis of target mRNA of differentially expressed miRNA revealed that mir-140-5p and mir-378-12 targeted corresponding genes to regulate NF-kappa B signaling pathway. JAK-STAT signaling pathway could be modulated by mir-425-2, mir-139-4, mir-140-6, mir-145-3, mir-140-5p and mir-204-2. This study compared the influence of miRNA-mRNA expression in Mv. l. Lu cells before and after CDV infection by integrated analysis of miRNA-mRNA and analyzed the complex network interaction between virus and host cells. The results can help understand the molecular mechanism of the natural immune response induced by CDV infection in host cells.https://www.frontiersin.org/articles/10.3389/fvets.2022.897740/fullcanine distemperMv. l. Lu cellsmiRNAtranscriptomecorrelation analysis |
| spellingShingle | Qiang Chen Qiang Chen Qiang Chen Mingwei Tong Na Sun Yong Yang Yuening Cheng Li Yi Gaili Wang Zhigang Cao Quan Zhao Shipeng Cheng Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper Virus Frontiers in Veterinary Science canine distemper Mv. l. Lu cells miRNA transcriptome correlation analysis |
| title | Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper Virus |
| title_full | Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper Virus |
| title_fullStr | Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper Virus |
| title_full_unstemmed | Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper Virus |
| title_short | Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper Virus |
| title_sort | integrated analysis of mirna mrna expression in mink lung epithelial cells infected with canine distemper virus |
| topic | canine distemper Mv. l. Lu cells miRNA transcriptome correlation analysis |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.897740/full |
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