Polymyxin E Induces Rapid Paenibacillus polymyxa Death by Damaging Cell Membrane while Ca2+ Can Protect Cells from Damage.
Polymyxin E, produced by Paenibacillus polymyxa, is an important antibiotic normally against Gram-negative pathogens. In this study, we found that polymyxin E can kill its producer P. polymyxa, a Gram-positive bacterium, by disrupting its cell membrane. Membrane damage was clearly revealed by detect...
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| 格式: | Article |
| 語言: | English |
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Public Library of Science (PLoS)
2015-01-01
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| 叢編: | PLoS ONE |
| 在線閱讀: | http://europepmc.org/articles/PMC4529208?pdf=render |
| _version_ | 1828259081541910528 |
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| author | Zhiliang Yu Yuanning Cai Wangrong Qin Jianxun Lin Juanping Qiu |
| author_facet | Zhiliang Yu Yuanning Cai Wangrong Qin Jianxun Lin Juanping Qiu |
| author_sort | Zhiliang Yu |
| collection | DOAJ |
| description | Polymyxin E, produced by Paenibacillus polymyxa, is an important antibiotic normally against Gram-negative pathogens. In this study, we found that polymyxin E can kill its producer P. polymyxa, a Gram-positive bacterium, by disrupting its cell membrane. Membrane damage was clearly revealed by detecting the leakage of intracellular molecules. The observation using scanning electron microscopy also supported that polymyxin E can destroy the cell membrane and cause an extensive cell surface alteration. On the other hand, divalent cations can give protection against polymyxin E. Compared with Mg2+, Ca2+ can more effectively alleviate polymyxin E-induced damage to the cell membrane, thus remarkably increasing the P. polymyxa survival. Our findings would shed light on a not yet described bactericidal mechanism of polymyxin E against Gram-positive bacteria and more importantly the nature of limited fermentation output of polymyxin E from P. polymyxa. |
| first_indexed | 2024-04-13T03:03:35Z |
| format | Article |
| id | doaj.art-ccb4e0a2c6b64d25a237a2d05bd0a27f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T03:03:35Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ccb4e0a2c6b64d25a237a2d05bd0a27f2022-12-22T03:05:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013519810.1371/journal.pone.0135198Polymyxin E Induces Rapid Paenibacillus polymyxa Death by Damaging Cell Membrane while Ca2+ Can Protect Cells from Damage.Zhiliang YuYuanning CaiWangrong QinJianxun LinJuanping QiuPolymyxin E, produced by Paenibacillus polymyxa, is an important antibiotic normally against Gram-negative pathogens. In this study, we found that polymyxin E can kill its producer P. polymyxa, a Gram-positive bacterium, by disrupting its cell membrane. Membrane damage was clearly revealed by detecting the leakage of intracellular molecules. The observation using scanning electron microscopy also supported that polymyxin E can destroy the cell membrane and cause an extensive cell surface alteration. On the other hand, divalent cations can give protection against polymyxin E. Compared with Mg2+, Ca2+ can more effectively alleviate polymyxin E-induced damage to the cell membrane, thus remarkably increasing the P. polymyxa survival. Our findings would shed light on a not yet described bactericidal mechanism of polymyxin E against Gram-positive bacteria and more importantly the nature of limited fermentation output of polymyxin E from P. polymyxa.http://europepmc.org/articles/PMC4529208?pdf=render |
| spellingShingle | Zhiliang Yu Yuanning Cai Wangrong Qin Jianxun Lin Juanping Qiu Polymyxin E Induces Rapid Paenibacillus polymyxa Death by Damaging Cell Membrane while Ca2+ Can Protect Cells from Damage. PLoS ONE |
| title | Polymyxin E Induces Rapid Paenibacillus polymyxa Death by Damaging Cell Membrane while Ca2+ Can Protect Cells from Damage. |
| title_full | Polymyxin E Induces Rapid Paenibacillus polymyxa Death by Damaging Cell Membrane while Ca2+ Can Protect Cells from Damage. |
| title_fullStr | Polymyxin E Induces Rapid Paenibacillus polymyxa Death by Damaging Cell Membrane while Ca2+ Can Protect Cells from Damage. |
| title_full_unstemmed | Polymyxin E Induces Rapid Paenibacillus polymyxa Death by Damaging Cell Membrane while Ca2+ Can Protect Cells from Damage. |
| title_short | Polymyxin E Induces Rapid Paenibacillus polymyxa Death by Damaging Cell Membrane while Ca2+ Can Protect Cells from Damage. |
| title_sort | polymyxin e induces rapid paenibacillus polymyxa death by damaging cell membrane while ca2 can protect cells from damage |
| url | http://europepmc.org/articles/PMC4529208?pdf=render |
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