Determining Composition of Volatiles in Couroupita guianensis Aubl. Through Headspace-Solid Phase Micro-Extraction (HS-SPME)
Composition of volatile components in Couroupita guianensis Aubl. flowers was analyzed using headspace-solid phase micro-extraction (HS-SPME), followed by capillary gas chromatography and mass spectrometry (GC-MS) separation and identification. In all, 75 compounds were identified accounting for 96...
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| Format: | Article |
| Language: | English |
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Society for Promotion of Horticulture - Indian Institute of Horticultural Research
2014-12-01
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| Series: | Journal of Horticultural Sciences |
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| Online Access: | https://jhs.iihr.res.in/index.php/jhs/article/view/188 |
| _version_ | 1797740681259646976 |
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| author | Arpita Mandal Khan K S Shivashankara T K Roy |
| author_facet | Arpita Mandal Khan K S Shivashankara T K Roy |
| author_sort | Arpita Mandal Khan |
| collection | DOAJ |
| description |
Composition of volatile components in Couroupita guianensis Aubl. flowers was analyzed using headspace-solid phase micro-extraction (HS-SPME), followed by capillary gas chromatography and mass spectrometry (GC-MS) separation and identification. In all, 75 compounds were identified accounting for 96.32% of the total volatiles present. The major groups of compounds present were oxygenated terpenoids (35.66%), alcohols (26.92%), esters (17.36%), mono-and sesqui-terpenoids (8.64%), aldehydes and ketones (4.71%), hydrocarbons (1.68%), phenols (0.18%), acids (0.754%) and heterocyclic compounds (0.42%) constituted a small proportion of the volatile profile. The most abundant individual constituent was eugenol (18.95%) followed by nerol (13.49%), (E,E) farnesol (12.88%), (E,E)-farnesyl acetate (6.68%), trans ocimene (6.02%), nootkatone (4.64%), geraniol (2.94%), 2-isopropenyl-5- methyl-4-hexenyl acetate (2.69%), cedr-8-en-13-ol (2.58%), (E,Z)-farnesyl acetate (2.40%) and methyl (11E)-11- hexadecenoate (2.041%). Analytical comparison of composition of volatiles in the flowers, obtained by different methods of extraction, viz., solvent extraction, micro-simultaneous extraction and headspace-solid phase microextraction, revealed specific variations in relative concentrations of the constituent chemicals. Linalool was the major chemical (21.5% and 14.9%) in solvent extract and micro-simultaneous extract, respectively, but appeared in negligible quantity (0.16%) in head-space analysis.
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| first_indexed | 2024-03-12T14:15:53Z |
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| id | doaj.art-ccb7563bcfd044ce99eabca403eb799e |
| institution | Directory Open Access Journal |
| issn | 0973-354X 2582-4899 |
| language | English |
| last_indexed | 2024-03-12T14:15:53Z |
| publishDate | 2014-12-01 |
| publisher | Society for Promotion of Horticulture - Indian Institute of Horticultural Research |
| record_format | Article |
| series | Journal of Horticultural Sciences |
| spelling | doaj.art-ccb7563bcfd044ce99eabca403eb799e2023-08-20T14:00:32ZengSociety for Promotion of Horticulture - Indian Institute of Horticultural ResearchJournal of Horticultural Sciences0973-354X2582-48992014-12-019210.24154/jhs.v9i2.188178Determining Composition of Volatiles in Couroupita guianensis Aubl. Through Headspace-Solid Phase Micro-Extraction (HS-SPME)Arpita Mandal KhanK S ShivashankaraT K Roy Composition of volatile components in Couroupita guianensis Aubl. flowers was analyzed using headspace-solid phase micro-extraction (HS-SPME), followed by capillary gas chromatography and mass spectrometry (GC-MS) separation and identification. In all, 75 compounds were identified accounting for 96.32% of the total volatiles present. The major groups of compounds present were oxygenated terpenoids (35.66%), alcohols (26.92%), esters (17.36%), mono-and sesqui-terpenoids (8.64%), aldehydes and ketones (4.71%), hydrocarbons (1.68%), phenols (0.18%), acids (0.754%) and heterocyclic compounds (0.42%) constituted a small proportion of the volatile profile. The most abundant individual constituent was eugenol (18.95%) followed by nerol (13.49%), (E,E) farnesol (12.88%), (E,E)-farnesyl acetate (6.68%), trans ocimene (6.02%), nootkatone (4.64%), geraniol (2.94%), 2-isopropenyl-5- methyl-4-hexenyl acetate (2.69%), cedr-8-en-13-ol (2.58%), (E,Z)-farnesyl acetate (2.40%) and methyl (11E)-11- hexadecenoate (2.041%). Analytical comparison of composition of volatiles in the flowers, obtained by different methods of extraction, viz., solvent extraction, micro-simultaneous extraction and headspace-solid phase microextraction, revealed specific variations in relative concentrations of the constituent chemicals. Linalool was the major chemical (21.5% and 14.9%) in solvent extract and micro-simultaneous extract, respectively, but appeared in negligible quantity (0.16%) in head-space analysis. https://jhs.iihr.res.in/index.php/jhs/article/view/188Couroupita guianensisVolatilesHeadspace-Solid Phase Micro-Extraction (HS-SPME)Capillary Gas Chromatography and Mass Spectrometry (GC/MS) |
| spellingShingle | Arpita Mandal Khan K S Shivashankara T K Roy Determining Composition of Volatiles in Couroupita guianensis Aubl. Through Headspace-Solid Phase Micro-Extraction (HS-SPME) Journal of Horticultural Sciences Couroupita guianensis Volatiles Headspace-Solid Phase Micro-Extraction (HS-SPME) Capillary Gas Chromatography and Mass Spectrometry (GC/MS) |
| title | Determining Composition of Volatiles in Couroupita guianensis Aubl. Through Headspace-Solid Phase Micro-Extraction (HS-SPME) |
| title_full | Determining Composition of Volatiles in Couroupita guianensis Aubl. Through Headspace-Solid Phase Micro-Extraction (HS-SPME) |
| title_fullStr | Determining Composition of Volatiles in Couroupita guianensis Aubl. Through Headspace-Solid Phase Micro-Extraction (HS-SPME) |
| title_full_unstemmed | Determining Composition of Volatiles in Couroupita guianensis Aubl. Through Headspace-Solid Phase Micro-Extraction (HS-SPME) |
| title_short | Determining Composition of Volatiles in Couroupita guianensis Aubl. Through Headspace-Solid Phase Micro-Extraction (HS-SPME) |
| title_sort | determining composition of volatiles in couroupita guianensis aubl through headspace solid phase micro extraction hs spme |
| topic | Couroupita guianensis Volatiles Headspace-Solid Phase Micro-Extraction (HS-SPME) Capillary Gas Chromatography and Mass Spectrometry (GC/MS) |
| url | https://jhs.iihr.res.in/index.php/jhs/article/view/188 |
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