Zinc Finger Nucleases as tools to understand and treat human diseases
<p>Abstract</p> <p>Recent work has shown that it is possible to target regulatory elements to DNA sequences of an investigator's choosing, increasing the armamentarium for probing gene function. In this review, we discuss the development and use of designer zinc finger protein...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2010-07-01
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| Series: | BMC Medicine |
| Online Access: | http://www.biomedcentral.com/1741-7015/8/42 |
| _version_ | 1811248903432962048 |
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| author | Stokoe David Davis David |
| author_facet | Stokoe David Davis David |
| author_sort | Stokoe David |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Recent work has shown that it is possible to target regulatory elements to DNA sequences of an investigator's choosing, increasing the armamentarium for probing gene function. In this review, we discuss the development and use of designer zinc finger proteins (ZFPs) as sequence specific tools. While the main focus of this review is to discuss the attachment of the FokI nuclease to ZFPs and the ability of the resulting fusion protein (termed zinc finger nucleases (ZFNs)) to genomically manipulate a gene of interest, we will also cover the utility of other functional domains, such as transcriptional activators and repressors, and highlight how these are being used as discovery and therapeutic tools.</p> |
| first_indexed | 2024-04-12T15:36:04Z |
| format | Article |
| id | doaj.art-ccb75faf04a145239c2610e6bc319b04 |
| institution | Directory Open Access Journal |
| issn | 1741-7015 |
| language | English |
| last_indexed | 2024-04-12T15:36:04Z |
| publishDate | 2010-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medicine |
| spelling | doaj.art-ccb75faf04a145239c2610e6bc319b042022-12-22T03:26:57ZengBMCBMC Medicine1741-70152010-07-01814210.1186/1741-7015-8-42Zinc Finger Nucleases as tools to understand and treat human diseasesStokoe DavidDavis David<p>Abstract</p> <p>Recent work has shown that it is possible to target regulatory elements to DNA sequences of an investigator's choosing, increasing the armamentarium for probing gene function. In this review, we discuss the development and use of designer zinc finger proteins (ZFPs) as sequence specific tools. While the main focus of this review is to discuss the attachment of the FokI nuclease to ZFPs and the ability of the resulting fusion protein (termed zinc finger nucleases (ZFNs)) to genomically manipulate a gene of interest, we will also cover the utility of other functional domains, such as transcriptional activators and repressors, and highlight how these are being used as discovery and therapeutic tools.</p>http://www.biomedcentral.com/1741-7015/8/42 |
| spellingShingle | Stokoe David Davis David Zinc Finger Nucleases as tools to understand and treat human diseases BMC Medicine |
| title | Zinc Finger Nucleases as tools to understand and treat human diseases |
| title_full | Zinc Finger Nucleases as tools to understand and treat human diseases |
| title_fullStr | Zinc Finger Nucleases as tools to understand and treat human diseases |
| title_full_unstemmed | Zinc Finger Nucleases as tools to understand and treat human diseases |
| title_short | Zinc Finger Nucleases as tools to understand and treat human diseases |
| title_sort | zinc finger nucleases as tools to understand and treat human diseases |
| url | http://www.biomedcentral.com/1741-7015/8/42 |
| work_keys_str_mv | AT stokoedavid zincfingernucleasesastoolstounderstandandtreathumandiseases AT davisdavid zincfingernucleasesastoolstounderstandandtreathumandiseases |