Effect of crocin, an active saffron constituent, on ethanol toxicity in the rat: histopathological and biochemical studies
<em><strong>Objective(s):</strong></em> The drinking of ethanol causes the wide range of clinical illness and morphological changes including hepatotoxicity and nephrotoxicity. In the current study, the protective properties of crocin versus oxidative stress, apoptosis and in...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-01-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_14140_f4c6aa580195ff56aa5ad313321c03fc.pdf |
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| author | Alireza Rezaee Khorasany Marjan Razavi Elahe Taghiabadi Abbass Tabatabaee Yazdy Hossein Hosseinzadeh |
| author_facet | Alireza Rezaee Khorasany Marjan Razavi Elahe Taghiabadi Abbass Tabatabaee Yazdy Hossein Hosseinzadeh |
| author_sort | Alireza Rezaee Khorasany |
| collection | DOAJ |
| description | <em><strong>Objective(s):</strong></em> The drinking of ethanol causes the wide range of clinical illness and morphological changes including hepatotoxicity and nephrotoxicity. In the current study, the protective properties of crocin versus oxidative stress, apoptosis and inflammation induced by ethanol were assessed. <br /><em><strong>Materials and Methods:</strong> </em>The male Wistar rats were divided into seven groups consisting of 6 rats in control, ethanol (50% v/v - 5 g/kg), crocin (10, 20 and 40 mg/Kg) plus ethanol, crocin 20 and 40 mg/Kg.<br /><em><strong>Results:</strong></em> The MDA level was remarkably enhanced, while the content of GSH was significantly diminished in the kidney and liver of alcoholic rat but protective groups restored the level of MDA and GSH contents. Ethanol consumption induced hepatotoxicity and nephrotoxicity as evidenced by biochemical abnormalities and histopathological damages but crocin improved them. Also, crocin restored the TNF-α and IL-6 levels in the liver. The consumption of ethanol enhanced the levels of caspase-3, -8, -9 and Bax/Bcl-2 ratio (mRNA and protein) but, western blot and real-time PCR data confirmed that crocin treatment prevented apoptosis induced by ethanol.<br /><em><strong>Conclusion:</strong> </em>This research demonstrates that crocin has protective activities against ethanol toxic effects in rat liver and kidney via anti-inflammatory, antioxidant, and anti-apoptotic effects. |
| first_indexed | 2024-12-13T18:37:04Z |
| format | Article |
| id | doaj.art-ccb775cdeb4a48a38d732faf96c2ab9f |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-13T18:37:04Z |
| publishDate | 2020-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-ccb775cdeb4a48a38d732faf96c2ab9f2022-12-21T23:35:20ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742020-01-01231516210.22038/ijbms.2019.37133.884514140Effect of crocin, an active saffron constituent, on ethanol toxicity in the rat: histopathological and biochemical studiesAlireza Rezaee Khorasany0Marjan Razavi1Elahe Taghiabadi2Abbass Tabatabaee Yazdy3Hossein Hosseinzadeh4Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranTargeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranOrthopedic Research Center, Shahid Kamyab Hospital, Mashhad University of Medical Sciences, Mashhad, IranGhaem Hospital, Department of Pathology, Mashhad University of Medical Sciences, Mashhad, IranPharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran<em><strong>Objective(s):</strong></em> The drinking of ethanol causes the wide range of clinical illness and morphological changes including hepatotoxicity and nephrotoxicity. In the current study, the protective properties of crocin versus oxidative stress, apoptosis and inflammation induced by ethanol were assessed. <br /><em><strong>Materials and Methods:</strong> </em>The male Wistar rats were divided into seven groups consisting of 6 rats in control, ethanol (50% v/v - 5 g/kg), crocin (10, 20 and 40 mg/Kg) plus ethanol, crocin 20 and 40 mg/Kg.<br /><em><strong>Results:</strong></em> The MDA level was remarkably enhanced, while the content of GSH was significantly diminished in the kidney and liver of alcoholic rat but protective groups restored the level of MDA and GSH contents. Ethanol consumption induced hepatotoxicity and nephrotoxicity as evidenced by biochemical abnormalities and histopathological damages but crocin improved them. Also, crocin restored the TNF-α and IL-6 levels in the liver. The consumption of ethanol enhanced the levels of caspase-3, -8, -9 and Bax/Bcl-2 ratio (mRNA and protein) but, western blot and real-time PCR data confirmed that crocin treatment prevented apoptosis induced by ethanol.<br /><em><strong>Conclusion:</strong> </em>This research demonstrates that crocin has protective activities against ethanol toxic effects in rat liver and kidney via anti-inflammatory, antioxidant, and anti-apoptotic effects.http://ijbms.mums.ac.ir/article_14140_f4c6aa580195ff56aa5ad313321c03fc.pdfcrocinethanolhepatotoxicity nephrotoxicityoxidative stress |
| spellingShingle | Alireza Rezaee Khorasany Marjan Razavi Elahe Taghiabadi Abbass Tabatabaee Yazdy Hossein Hosseinzadeh Effect of crocin, an active saffron constituent, on ethanol toxicity in the rat: histopathological and biochemical studies Iranian Journal of Basic Medical Sciences crocin ethanol hepatotoxicity nephrotoxicity oxidative stress |
| title | Effect of crocin, an active saffron constituent, on ethanol toxicity in the rat: histopathological and biochemical studies |
| title_full | Effect of crocin, an active saffron constituent, on ethanol toxicity in the rat: histopathological and biochemical studies |
| title_fullStr | Effect of crocin, an active saffron constituent, on ethanol toxicity in the rat: histopathological and biochemical studies |
| title_full_unstemmed | Effect of crocin, an active saffron constituent, on ethanol toxicity in the rat: histopathological and biochemical studies |
| title_short | Effect of crocin, an active saffron constituent, on ethanol toxicity in the rat: histopathological and biochemical studies |
| title_sort | effect of crocin an active saffron constituent on ethanol toxicity in the rat histopathological and biochemical studies |
| topic | crocin ethanol hepatotoxicity nephrotoxicity oxidative stress |
| url | http://ijbms.mums.ac.ir/article_14140_f4c6aa580195ff56aa5ad313321c03fc.pdf |
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