Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia.
Clinical evidence has shown that stress may be associated with alterations in masticatory muscle functions. Morphological changes in masticatory muscles induced by occlusal alterations and associated with emotional stress are still lacking in the literature. The objective of this study was to evalua...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4459827?pdf=render |
| _version_ | 1818399417088081920 |
|---|---|
| author | Mamie Mizusaki Iyomasa Fernanda Silva Fernandes Daniela Mizusaki Iyomasa Yamba Carla Lara Pereira Rodrigo Alberto Restrepo Fernández Ricardo Alexandre Calzzani Glauce Crivelaro Nascimento Christie Ramos Andrade Leite-Panissi João Paulo Mardegan Issa |
| author_facet | Mamie Mizusaki Iyomasa Fernanda Silva Fernandes Daniela Mizusaki Iyomasa Yamba Carla Lara Pereira Rodrigo Alberto Restrepo Fernández Ricardo Alexandre Calzzani Glauce Crivelaro Nascimento Christie Ramos Andrade Leite-Panissi João Paulo Mardegan Issa |
| author_sort | Mamie Mizusaki Iyomasa |
| collection | DOAJ |
| description | Clinical evidence has shown that stress may be associated with alterations in masticatory muscle functions. Morphological changes in masticatory muscles induced by occlusal alterations and associated with emotional stress are still lacking in the literature. The objective of this study was to evaluate the influence of acute stress on metabolic activity and oxidative stress of masseter muscles of rats subjected to occlusal modification through morphological and histochemical analyses. In this study, adult Wistar rats were divided into 4 groups: a group with extraction and acute stress (E+A); group with extraction and without stress (E+C); group without extraction and with acute stress (NO+A); and control group without both extraction and stress (NO+C). Masseter muscles were analyzed by Succinate Dehydrogenase (SDH), Nicotinamide Adenine Dinucleotide Diaphorase (NADH) and Reactive Oxygen Species (ROS) techniques. Statistical analyses and two-way ANOVA were applied, followed by Tukey-Kramer tests. In the SDH test, the E+C, E+A and NO+A groups showed a decrease in high desidrogenase activities fibers (P < 0.05), compared to the NO+C group. In the NADH test, there was no difference among the different groups. In the ROS test, in contrast, E+A, E+C and NO+A groups showed a decrease in ROS expression, compared to NO+C groups (P < 0.05). Modified dental occlusion and acute stress--which are important and prevalent problems that affect the general population--are important etiologic factors in metabolic plasticity and ROS levels of masseter muscles. |
| first_indexed | 2024-12-14T07:20:21Z |
| format | Article |
| id | doaj.art-ccb8614b608341a1aa34deebb86bc6e4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T07:20:21Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ccb8614b608341a1aa34deebb86bc6e42022-12-21T23:11:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e012839710.1371/journal.pone.0128397Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia.Mamie Mizusaki IyomasaFernanda Silva FernandesDaniela Mizusaki IyomasaYamba Carla Lara PereiraRodrigo Alberto Restrepo FernándezRicardo Alexandre CalzzaniGlauce Crivelaro NascimentoChristie Ramos Andrade Leite-PanissiJoão Paulo Mardegan IssaClinical evidence has shown that stress may be associated with alterations in masticatory muscle functions. Morphological changes in masticatory muscles induced by occlusal alterations and associated with emotional stress are still lacking in the literature. The objective of this study was to evaluate the influence of acute stress on metabolic activity and oxidative stress of masseter muscles of rats subjected to occlusal modification through morphological and histochemical analyses. In this study, adult Wistar rats were divided into 4 groups: a group with extraction and acute stress (E+A); group with extraction and without stress (E+C); group without extraction and with acute stress (NO+A); and control group without both extraction and stress (NO+C). Masseter muscles were analyzed by Succinate Dehydrogenase (SDH), Nicotinamide Adenine Dinucleotide Diaphorase (NADH) and Reactive Oxygen Species (ROS) techniques. Statistical analyses and two-way ANOVA were applied, followed by Tukey-Kramer tests. In the SDH test, the E+C, E+A and NO+A groups showed a decrease in high desidrogenase activities fibers (P < 0.05), compared to the NO+C group. In the NADH test, there was no difference among the different groups. In the ROS test, in contrast, E+A, E+C and NO+A groups showed a decrease in ROS expression, compared to NO+C groups (P < 0.05). Modified dental occlusion and acute stress--which are important and prevalent problems that affect the general population--are important etiologic factors in metabolic plasticity and ROS levels of masseter muscles.http://europepmc.org/articles/PMC4459827?pdf=render |
| spellingShingle | Mamie Mizusaki Iyomasa Fernanda Silva Fernandes Daniela Mizusaki Iyomasa Yamba Carla Lara Pereira Rodrigo Alberto Restrepo Fernández Ricardo Alexandre Calzzani Glauce Crivelaro Nascimento Christie Ramos Andrade Leite-Panissi João Paulo Mardegan Issa Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia. PLoS ONE |
| title | Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia. |
| title_full | Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia. |
| title_fullStr | Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia. |
| title_full_unstemmed | Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia. |
| title_short | Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia. |
| title_sort | metabolic changes in masseter muscle of rats submitted to acute stress associated with exodontia |
| url | http://europepmc.org/articles/PMC4459827?pdf=render |
| work_keys_str_mv | AT mamiemizusakiiyomasa metabolicchangesinmassetermuscleofratssubmittedtoacutestressassociatedwithexodontia AT fernandasilvafernandes metabolicchangesinmassetermuscleofratssubmittedtoacutestressassociatedwithexodontia AT danielamizusakiiyomasa metabolicchangesinmassetermuscleofratssubmittedtoacutestressassociatedwithexodontia AT yambacarlalarapereira metabolicchangesinmassetermuscleofratssubmittedtoacutestressassociatedwithexodontia AT rodrigoalbertorestrepofernandez metabolicchangesinmassetermuscleofratssubmittedtoacutestressassociatedwithexodontia AT ricardoalexandrecalzzani metabolicchangesinmassetermuscleofratssubmittedtoacutestressassociatedwithexodontia AT glaucecrivelaronascimento metabolicchangesinmassetermuscleofratssubmittedtoacutestressassociatedwithexodontia AT christieramosandradeleitepanissi metabolicchangesinmassetermuscleofratssubmittedtoacutestressassociatedwithexodontia AT joaopaulomardeganissa metabolicchangesinmassetermuscleofratssubmittedtoacutestressassociatedwithexodontia |