Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation
Abstract Metataxonomic approach was used to describe the bacterial community from a creosote-contaminated aquifer and to access the potential for in situ bioremediation of the polycyclic aromatic hydrocarbons (PAHs) by biostimulation. In general, the wells with higher PAH contamination had lower ric...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2019-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-019-47921-y |
| _version_ | 1830144373636988928 |
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| author | Aline Daniela Lopes Júlio Ubiana de Cássia Mourão Silva Julliane Dutra Medeiros Daniel Kumazawa Morais Vera Lúcia dos Santos |
| author_facet | Aline Daniela Lopes Júlio Ubiana de Cássia Mourão Silva Julliane Dutra Medeiros Daniel Kumazawa Morais Vera Lúcia dos Santos |
| author_sort | Aline Daniela Lopes Júlio |
| collection | DOAJ |
| description | Abstract Metataxonomic approach was used to describe the bacterial community from a creosote-contaminated aquifer and to access the potential for in situ bioremediation of the polycyclic aromatic hydrocarbons (PAHs) by biostimulation. In general, the wells with higher PAH contamination had lower richness and diversity than others, using the Shannon and Simpson indices. By the principal coordinate analysis (PCoA) it was possible to observe the clustering of the bacterial community of most wells in response of the presence of PAH contamination. The significance analysis using edgeR package of the R program showed variation in the abundance of some Operational Taxonomic Units (OTUs) of contaminated wells compared to uncontaminated ones. Taxons enriched in the contaminated wells were correlated positively (p < 0.05) with the hydrocarbons, according to redundancy analysis (RDA). All these enriched taxa have been characterized as PAH degrading agents, such as the genus Comamonas, Geobacter, Hydrocarboniphaga, Anaerolinea and Desulfomonile. Additionally, it was possible to predict, with the PICRUSt program, a greater proportion of pathways and genes related to the degradation of PAHs in the wells with higher contamination levels. We conclude that the contaminants promoted the enrichment of several groups of degrading bacteria in the area, which strengthens the feasibility of applying biostimulation as an aquifer remediation strategy. |
| first_indexed | 2024-12-17T10:05:09Z |
| format | Article |
| id | doaj.art-ccbb06c139294f3cad67e7c78994b2b7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T10:05:09Z |
| publishDate | 2019-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ccbb06c139294f3cad67e7c78994b2b72022-12-21T21:53:11ZengNature PortfolioScientific Reports2045-23222019-08-019111410.1038/s41598-019-47921-yMetataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediationAline Daniela Lopes Júlio0Ubiana de Cássia Mourão Silva1Julliane Dutra Medeiros2Daniel Kumazawa Morais3Vera Lúcia dos Santos4Laboratory of Applied Microbiology, Microbiology Department, Institute of Biological Science, Universidade Federal de Minas GeraisLaboratory of Applied Microbiology, Microbiology Department, Institute of Biological Science, Universidade Federal de Minas GeraisBiosystems Informatics and Genomics Group, René Rachou Research Center/Fiocruz-MGLaboratory of Environmental Microbiology, Institute of Microbiology of the Czech Academy of Sciences – CASLaboratory of Applied Microbiology, Microbiology Department, Institute of Biological Science, Universidade Federal de Minas GeraisAbstract Metataxonomic approach was used to describe the bacterial community from a creosote-contaminated aquifer and to access the potential for in situ bioremediation of the polycyclic aromatic hydrocarbons (PAHs) by biostimulation. In general, the wells with higher PAH contamination had lower richness and diversity than others, using the Shannon and Simpson indices. By the principal coordinate analysis (PCoA) it was possible to observe the clustering of the bacterial community of most wells in response of the presence of PAH contamination. The significance analysis using edgeR package of the R program showed variation in the abundance of some Operational Taxonomic Units (OTUs) of contaminated wells compared to uncontaminated ones. Taxons enriched in the contaminated wells were correlated positively (p < 0.05) with the hydrocarbons, according to redundancy analysis (RDA). All these enriched taxa have been characterized as PAH degrading agents, such as the genus Comamonas, Geobacter, Hydrocarboniphaga, Anaerolinea and Desulfomonile. Additionally, it was possible to predict, with the PICRUSt program, a greater proportion of pathways and genes related to the degradation of PAHs in the wells with higher contamination levels. We conclude that the contaminants promoted the enrichment of several groups of degrading bacteria in the area, which strengthens the feasibility of applying biostimulation as an aquifer remediation strategy.https://doi.org/10.1038/s41598-019-47921-y |
| spellingShingle | Aline Daniela Lopes Júlio Ubiana de Cássia Mourão Silva Julliane Dutra Medeiros Daniel Kumazawa Morais Vera Lúcia dos Santos Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation Scientific Reports |
| title | Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation |
| title_full | Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation |
| title_fullStr | Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation |
| title_full_unstemmed | Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation |
| title_short | Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation |
| title_sort | metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation |
| url | https://doi.org/10.1038/s41598-019-47921-y |
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