Substrate Shift Reveals Roles for Members of Bacterial Consortia in Degradation of Plant Cell Wall Polymers
Deconstructing the intricate matrix of cellulose, hemicellulose, and lignin poses a major challenge in biofuel production. In diverse environments in nature, some microbial communities, are able to overcome plant biomass recalcitrance. Identifying key degraders of each component of plant cell wall c...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00364/full |
| _version_ | 1811327699676823552 |
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| author | Camila Carlos Camila Carlos Huan Fan Cameron R. Currie Cameron R. Currie |
| author_facet | Camila Carlos Camila Carlos Huan Fan Cameron R. Currie Cameron R. Currie |
| author_sort | Camila Carlos |
| collection | DOAJ |
| description | Deconstructing the intricate matrix of cellulose, hemicellulose, and lignin poses a major challenge in biofuel production. In diverse environments in nature, some microbial communities, are able to overcome plant biomass recalcitrance. Identifying key degraders of each component of plant cell wall can help improve biological degradation of plant feedstock. Here, we sequenced the metagenome of lignocellulose-adapted microbial consortia sub-cultured on xylan and alkali lignin media. We observed a drastic shift on community composition after sub-culturing, independently of the original consortia. Proteobacteria relative abundance increased after growth in alkali lignin medium, while Bacteroidetes abundance increased after growth in xylan medium. At the genus level, Pseudomonas was more abundant in the communities growing on alkali lignin, Sphingobacterium in the communities growing on xylan and Cellulomonas abundance was the highest in the original microbial consortia. We also observed functional convergence of microbial communities after incubation in alkali lignin, due to an enrichment of genes involved in benzoate degradation and catechol ortho-cleavage pathways. Our results represent an important step toward the elucidation of key members of microbial communities on lignocellulose degradation and may aide the design of novel lignocellulolytic microbial consortia that are able to efficiently degrade plant cell wall polymers. |
| first_indexed | 2024-04-13T15:11:36Z |
| format | Article |
| id | doaj.art-ccbb51991fde46b0878ec7b6c802c3aa |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T15:11:36Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ccbb51991fde46b0878ec7b6c802c3aa2022-12-22T02:41:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-03-01910.3389/fmicb.2018.00364312542Substrate Shift Reveals Roles for Members of Bacterial Consortia in Degradation of Plant Cell Wall PolymersCamila Carlos0Camila Carlos1Huan Fan2Cameron R. Currie3Cameron R. Currie4Department of Bacteriology, University of Wisconsin–Madison, Madison, WI, United StatesU.S. Department of Energy, Great Lakes Bioenergy Research Center, University of Wisconsin–Madison, Madison, WI, United StatesDepartment of Bacteriology, University of Wisconsin–Madison, Madison, WI, United StatesDepartment of Bacteriology, University of Wisconsin–Madison, Madison, WI, United StatesU.S. Department of Energy, Great Lakes Bioenergy Research Center, University of Wisconsin–Madison, Madison, WI, United StatesDeconstructing the intricate matrix of cellulose, hemicellulose, and lignin poses a major challenge in biofuel production. In diverse environments in nature, some microbial communities, are able to overcome plant biomass recalcitrance. Identifying key degraders of each component of plant cell wall can help improve biological degradation of plant feedstock. Here, we sequenced the metagenome of lignocellulose-adapted microbial consortia sub-cultured on xylan and alkali lignin media. We observed a drastic shift on community composition after sub-culturing, independently of the original consortia. Proteobacteria relative abundance increased after growth in alkali lignin medium, while Bacteroidetes abundance increased after growth in xylan medium. At the genus level, Pseudomonas was more abundant in the communities growing on alkali lignin, Sphingobacterium in the communities growing on xylan and Cellulomonas abundance was the highest in the original microbial consortia. We also observed functional convergence of microbial communities after incubation in alkali lignin, due to an enrichment of genes involved in benzoate degradation and catechol ortho-cleavage pathways. Our results represent an important step toward the elucidation of key members of microbial communities on lignocellulose degradation and may aide the design of novel lignocellulolytic microbial consortia that are able to efficiently degrade plant cell wall polymers.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00364/fullmicrobial enrichmentlignocellulosexylanligninPseudomonasSphingobacterium |
| spellingShingle | Camila Carlos Camila Carlos Huan Fan Cameron R. Currie Cameron R. Currie Substrate Shift Reveals Roles for Members of Bacterial Consortia in Degradation of Plant Cell Wall Polymers Frontiers in Microbiology microbial enrichment lignocellulose xylan lignin Pseudomonas Sphingobacterium |
| title | Substrate Shift Reveals Roles for Members of Bacterial Consortia in Degradation of Plant Cell Wall Polymers |
| title_full | Substrate Shift Reveals Roles for Members of Bacterial Consortia in Degradation of Plant Cell Wall Polymers |
| title_fullStr | Substrate Shift Reveals Roles for Members of Bacterial Consortia in Degradation of Plant Cell Wall Polymers |
| title_full_unstemmed | Substrate Shift Reveals Roles for Members of Bacterial Consortia in Degradation of Plant Cell Wall Polymers |
| title_short | Substrate Shift Reveals Roles for Members of Bacterial Consortia in Degradation of Plant Cell Wall Polymers |
| title_sort | substrate shift reveals roles for members of bacterial consortia in degradation of plant cell wall polymers |
| topic | microbial enrichment lignocellulose xylan lignin Pseudomonas Sphingobacterium |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00364/full |
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