Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining
Examination of sperm morphology is an essential criterion for evaluating male fertility. This examination can be done by staining sperm cells with various techniques to facilitate the visualization of sperm cells. Several other methods that can be used to evaluate sperm morphology are Eosin-nigrosi...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Nahdlatul Ulama Surabaya
2023-08-01
|
| Series: | Indonesian Journal of Medical Laboratory Science and Technology |
| Subjects: | |
| Online Access: | https://103.106.72.77/index.php/IJMLST/article/view/3961 |
| _version_ | 1797733989890392064 |
|---|---|
| author | Mamay Mamay Ernawati Ernawati Astari Nurisani |
| author_facet | Mamay Mamay Ernawati Ernawati Astari Nurisani |
| author_sort | Mamay Mamay |
| collection | DOAJ |
| description |
Examination of sperm morphology is an essential criterion for evaluating male fertility. This examination can be done by staining sperm cells with various techniques to facilitate the visualization of sperm cells. Several other methods that can be used to evaluate sperm morphology are Eosin-nigrosin, AgNO3 staining, Papanicolaou, Diff Quick and Giemsa. However, using these synthetic dyes can harm the environment and water sources because they prefer to decompose. The natural dye derived from environmentally friendly plants expected to minimize hazardous waste. This study aimed to identify and compare the potency of several natural dyes derived from purple cabbage, sappan wood and beetroot, which have not been studied in sperm studies. Sperm obtained from 30 men in the campus area and underwent 3-5 days of abstinence from intercourse in preparation for sampling. The sperm stained with natural dye, and their quality compared with WHO standard Papanicolaou. Extracts of sappan wood, purple cabbage and beetroot are made with various mordant alum, ethanol and acetic acid compositions. The study's results showed that the presence of mordant increased the staining quality of sperm with sappan wood and purple cabbage extracts. The use of acid improves the quality of sperm staining with beet extract. There was no different between dyeing with beetroot extract in various solvent compositions and dyeing with Papanicolaou to color all sperm components (p> 0.05). The results showed that beetroot extract has a high potential to evaluate sperm morphology.
|
| first_indexed | 2024-03-12T12:36:38Z |
| format | Article |
| id | doaj.art-ccbcbb85cb8149f4bf0308dcd9cf25ce |
| institution | Directory Open Access Journal |
| issn | 2684-6748 2656-9825 |
| language | English |
| last_indexed | 2024-03-12T12:36:38Z |
| publishDate | 2023-08-01 |
| publisher | Universitas Nahdlatul Ulama Surabaya |
| record_format | Article |
| series | Indonesian Journal of Medical Laboratory Science and Technology |
| spelling | doaj.art-ccbcbb85cb8149f4bf0308dcd9cf25ce2023-08-29T00:13:40ZengUniversitas Nahdlatul Ulama SurabayaIndonesian Journal of Medical Laboratory Science and Technology2684-67482656-98252023-08-015210.33086/ijmlst.v5i2.3961Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm StainingMamay Mamay0Ernawati Ernawati1Astari Nurisani2Department of Medical Laboratory Technology, STIKes Karsa Husada, Garut, IndonesiaDepartment of Midwifery, STIKes Karsa Husada, Garut, IndonesiaDepartment of Medical Laboratory Technology, STIKes Karsa Husada, Garut, Indonesia Examination of sperm morphology is an essential criterion for evaluating male fertility. This examination can be done by staining sperm cells with various techniques to facilitate the visualization of sperm cells. Several other methods that can be used to evaluate sperm morphology are Eosin-nigrosin, AgNO3 staining, Papanicolaou, Diff Quick and Giemsa. However, using these synthetic dyes can harm the environment and water sources because they prefer to decompose. The natural dye derived from environmentally friendly plants expected to minimize hazardous waste. This study aimed to identify and compare the potency of several natural dyes derived from purple cabbage, sappan wood and beetroot, which have not been studied in sperm studies. Sperm obtained from 30 men in the campus area and underwent 3-5 days of abstinence from intercourse in preparation for sampling. The sperm stained with natural dye, and their quality compared with WHO standard Papanicolaou. Extracts of sappan wood, purple cabbage and beetroot are made with various mordant alum, ethanol and acetic acid compositions. The study's results showed that the presence of mordant increased the staining quality of sperm with sappan wood and purple cabbage extracts. The use of acid improves the quality of sperm staining with beet extract. There was no different between dyeing with beetroot extract in various solvent compositions and dyeing with Papanicolaou to color all sperm components (p> 0.05). The results showed that beetroot extract has a high potential to evaluate sperm morphology. https://103.106.72.77/index.php/IJMLST/article/view/3961BeetrootPurple CabbageSappan WoodSpermStaining |
| spellingShingle | Mamay Mamay Ernawati Ernawati Astari Nurisani Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining Indonesian Journal of Medical Laboratory Science and Technology Beetroot Purple Cabbage Sappan Wood Sperm Staining |
| title | Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining |
| title_full | Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining |
| title_fullStr | Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining |
| title_full_unstemmed | Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining |
| title_short | Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining |
| title_sort | potential of sappan wood purple cabbage and beetroot extract in sperm staining |
| topic | Beetroot Purple Cabbage Sappan Wood Sperm Staining |
| url | https://103.106.72.77/index.php/IJMLST/article/view/3961 |
| work_keys_str_mv | AT mamaymamay potentialofsappanwoodpurplecabbageandbeetrootextractinspermstaining AT ernawatiernawati potentialofsappanwoodpurplecabbageandbeetrootextractinspermstaining AT astarinurisani potentialofsappanwoodpurplecabbageandbeetrootextractinspermstaining |