| Summary: | Th is study aimed to investigate the eff ect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%)
lyophilized extender on freezability of ram semen. Collected semen was pooled and split into two aliquots, and each of the ejaculates was
diluted with fresh or lyophilized extenders with RTSP (0%, FC and LC; 10% F10 and L10or 15% F15 and L15) using two-step dilution
method. Semen was frozen using the programmable freezing machine. Semen samples were examined for sperm motility, defective
acrosomes, plasma membrane integrity and DNA fragmentation at native and post-thaw stage. Th e highest percentages of post-thaw
motility and plasma integrity were observed in the F10, F15, and L15 (P<0.05). Th ere was no significant diff erence in the rate of post-thaw
defective acrosomes when the other extender groups were compared with the control (P>0.05). Th e highest percentage of post-thaw DNA
fragmentation values were observed in the FC group, while the lowest DNA fragmentation was obtained in the F15 but only significant
diff erent from FC and LC groups. In conclusion, the findings of this study show that the lyophilized extender with 15% RTSP added can
be used successfully for freezing ram semen.
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