Lyophilized extender supplemented with rainbow trout (oncorhynchus mykiss) seminal plasma improves cryopreservation of ram sperm
Th is study aimed to investigate the eff ect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%) lyophilized extender on freezability of ram semen. Collected semen was pooled and split into two aliquots, and each of the ejaculates was diluted with fresh or lyophilized exten...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2022-03-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2899 |
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| author | Burcu USTUNER Selim ALCAY Elif GOKCE M. Melih YILMAZ Ahmet AKTAR Oguzhan HURAYDIN Muhammed DUMAN N. Tekin ONDER Eser AKAL Zekariya NUR |
| author_facet | Burcu USTUNER Selim ALCAY Elif GOKCE M. Melih YILMAZ Ahmet AKTAR Oguzhan HURAYDIN Muhammed DUMAN N. Tekin ONDER Eser AKAL Zekariya NUR |
| author_sort | Burcu USTUNER |
| collection | DOAJ |
| description | Th is study aimed to investigate the eff ect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%)
lyophilized extender on freezability of ram semen. Collected semen was pooled and split into two aliquots, and each of the ejaculates was
diluted with fresh or lyophilized extenders with RTSP (0%, FC and LC; 10% F10 and L10or 15% F15 and L15) using two-step dilution
method. Semen was frozen using the programmable freezing machine. Semen samples were examined for sperm motility, defective
acrosomes, plasma membrane integrity and DNA fragmentation at native and post-thaw stage. Th e highest percentages of post-thaw
motility and plasma integrity were observed in the F10, F15, and L15 (P<0.05). Th ere was no significant diff erence in the rate of post-thaw
defective acrosomes when the other extender groups were compared with the control (P>0.05). Th e highest percentage of post-thaw DNA
fragmentation values were observed in the FC group, while the lowest DNA fragmentation was obtained in the F15 but only significant
diff erent from FC and LC groups. In conclusion, the findings of this study show that the lyophilized extender with 15% RTSP added can
be used successfully for freezing ram semen. |
| first_indexed | 2024-03-13T04:39:43Z |
| format | Article |
| id | doaj.art-ccbe86cc24ac4607bc1ae10c15bd3678 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:39:43Z |
| publishDate | 2022-03-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-ccbe86cc24ac4607bc1ae10c15bd36782023-06-19T06:28:21ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512022-03-0128225526010.9775/kvfd.2021.268552899Lyophilized extender supplemented with rainbow trout (oncorhynchus mykiss) seminal plasma improves cryopreservation of ram spermBurcu USTUNER0Selim ALCAY1Elif GOKCE2M. Melih YILMAZ3Ahmet AKTAR4Oguzhan HURAYDIN5Muhammed DUMAN6N. Tekin ONDER7Eser AKAL8Zekariya NUR9Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, TR-16059 Görükle, Bursa - TÜRKİYEDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, TR-16059 Görükle, Bursa - TÜRKİYEDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Namik Kemal University, TR-59030 Tekirdağ - TÜRKİYEDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, TR-16059 Görükle, Bursa - TÜRKİYEDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, TR-16059 Görükle, Bursa - TÜRKİYEDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, TR-16059 Görükle, Bursa - TÜRKİYEDepartment of Aquatic Animal Disease, Faculty of Veterinary Medicine, Uludag University, TR-16059 Görükle, Bursa - TÜRKİYEDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Kafk as University, TR-36300 Paşaçayırı, Kars - TÜRKİYEDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ondokuz Mayis University, TR-55200 Atakum, Samsun - TÜRKİYEDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, TR-16059 Görükle, Bursa - TÜRKİYETh is study aimed to investigate the eff ect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%) lyophilized extender on freezability of ram semen. Collected semen was pooled and split into two aliquots, and each of the ejaculates was diluted with fresh or lyophilized extenders with RTSP (0%, FC and LC; 10% F10 and L10or 15% F15 and L15) using two-step dilution method. Semen was frozen using the programmable freezing machine. Semen samples were examined for sperm motility, defective acrosomes, plasma membrane integrity and DNA fragmentation at native and post-thaw stage. Th e highest percentages of post-thaw motility and plasma integrity were observed in the F10, F15, and L15 (P<0.05). Th ere was no significant diff erence in the rate of post-thaw defective acrosomes when the other extender groups were compared with the control (P>0.05). Th e highest percentage of post-thaw DNA fragmentation values were observed in the FC group, while the lowest DNA fragmentation was obtained in the F15 but only significant diff erent from FC and LC groups. In conclusion, the findings of this study show that the lyophilized extender with 15% RTSP added can be used successfully for freezing ram semen.https://vetdergikafkas.org/pdf.php?id=2899cryopreservationlyophilized extenderrainbow trout seminal plasmaram semen |
| spellingShingle | Burcu USTUNER Selim ALCAY Elif GOKCE M. Melih YILMAZ Ahmet AKTAR Oguzhan HURAYDIN Muhammed DUMAN N. Tekin ONDER Eser AKAL Zekariya NUR Lyophilized extender supplemented with rainbow trout (oncorhynchus mykiss) seminal plasma improves cryopreservation of ram sperm Kafkas Universitesi Veteriner Fakültesi Dergisi cryopreservation lyophilized extender rainbow trout seminal plasma ram semen |
| title | Lyophilized extender supplemented with rainbow trout (oncorhynchus mykiss) seminal plasma improves cryopreservation of ram sperm |
| title_full | Lyophilized extender supplemented with rainbow trout (oncorhynchus mykiss) seminal plasma improves cryopreservation of ram sperm |
| title_fullStr | Lyophilized extender supplemented with rainbow trout (oncorhynchus mykiss) seminal plasma improves cryopreservation of ram sperm |
| title_full_unstemmed | Lyophilized extender supplemented with rainbow trout (oncorhynchus mykiss) seminal plasma improves cryopreservation of ram sperm |
| title_short | Lyophilized extender supplemented with rainbow trout (oncorhynchus mykiss) seminal plasma improves cryopreservation of ram sperm |
| title_sort | lyophilized extender supplemented with rainbow trout oncorhynchus mykiss seminal plasma improves cryopreservation of ram sperm |
| topic | cryopreservation lyophilized extender rainbow trout seminal plasma ram semen |
| url | https://vetdergikafkas.org/pdf.php?id=2899 |
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