Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (<i>Chondrilla caribensis</i> and <i>Aplysina fulva</i>): Physicochemical Properties and In Vitro Biological Analysis
This study aimed to compare different protocols (Protocol 1: P1; Protocol 2: P2; Protocol 3: P3; Protocol 4: P4) for the extraction of spongin-like collagen (SC) from marine sponges. The SEM micrographs demonstrated a fibrillar structure for the extracts from <i>Chondrilla caribensis</i>...
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MDPI AG
2021-07-01
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author | Tiago A. T. Araújo Amanda de Souza Alan F. Santana Anna Rafaela C. Braga Márcio R. Custódio Fábio R. Simões Gabriela M. Araújo Antônio Miranda Flávio Alves Renata N. Granito Na Yu Ana Claudia M. Renno |
author_facet | Tiago A. T. Araújo Amanda de Souza Alan F. Santana Anna Rafaela C. Braga Márcio R. Custódio Fábio R. Simões Gabriela M. Araújo Antônio Miranda Flávio Alves Renata N. Granito Na Yu Ana Claudia M. Renno |
author_sort | Tiago A. T. Araújo |
collection | DOAJ |
description | This study aimed to compare different protocols (Protocol 1: P1; Protocol 2: P2; Protocol 3: P3; Protocol 4: P4) for the extraction of spongin-like collagen (SC) from marine sponges. The SEM micrographs demonstrated a fibrillar structure for the extracts from <i>Chondrilla caribensis</i> and the nodular/particulate aggregates for <i>Aplysina fulva</i>. FTIR showed for all samples peaks similar to collagen for both species. For <i>C. caribensis</i>, the extracts obtained using P2, P3, and P4 protocols presented higher values of extraction yield, TPQ, and GAGs. P2 and P4 showed higher values of SC concentration and for antioxidant analysis. For <i>A. fulva</i>, P2, P3, and P4 provided a higher extraction yield besides an increase in the antioxidant assay. For both species, no difference was observed for Col quantification and TPQ analysis; also, higher values of GAGs were found using P2 and P4. Fibroblast proliferation observed for <i>C. caribensis</i> was lower for P1 on day 1 and for P2 and P3 on day 3 (for 50%) compared to the control group. There was a significant reduction in fibroblast cell proliferation for all <i>A. fulva</i> extracts evaluated. It can be concluded that protocols P2 and P4 were more efficient for extracting SC from <i>C. caribensis</i>. |
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language | English |
last_indexed | 2024-03-10T09:32:46Z |
publishDate | 2021-07-01 |
publisher | MDPI AG |
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series | Membranes |
spelling | doaj.art-ccc17903e3534876b43e2050d71e28052023-11-22T04:22:16ZengMDPI AGMembranes2077-03752021-07-0111752210.3390/membranes11070522Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (<i>Chondrilla caribensis</i> and <i>Aplysina fulva</i>): Physicochemical Properties and In Vitro Biological AnalysisTiago A. T. Araújo0Amanda de Souza1Alan F. Santana2Anna Rafaela C. Braga3Márcio R. Custódio4Fábio R. Simões5Gabriela M. Araújo6Antônio Miranda7Flávio Alves8Renata N. Granito9Na Yu10Ana Claudia M. Renno11Department of Biosciences, Universidade Federal de São Paulo (UNIFESP), Santos 11015-220, SP, BrazilDepartment of Biosciences, Universidade Federal de São Paulo (UNIFESP), Santos 11015-220, SP, BrazilDepartment of Biosciences, Universidade Federal de São Paulo (UNIFESP), Santos 11015-220, SP, BrazilDepartment of Biosciences, Universidade Federal de São Paulo (UNIFESP), Santos 11015-220, SP, BrazilLaboratory of Marine Invertebrates Cell Biology, Institute of Biosciences, Universidade de São Paulo (USP), São Paulo 05508-090, SP, BrazilInstitute of Marine Sciences, Universidade Federal de São Paulo (UNIFESP), Santos 11070-100, SP, BrazilInstitute of Marine Sciences, Universidade Federal de São Paulo (UNIFESP), Santos 11070-100, SP, BrazilDepartment of Biophysics, Universidade Federal de São Paulo (UNIFESP), São Paulo 04044-020, SP, BrazilDepartment of Biophysics, Universidade Federal de São Paulo (UNIFESP), São Paulo 04044-020, SP, BrazilDepartment of Biosciences, Universidade Federal de São Paulo (UNIFESP), Santos 11015-220, SP, BrazilNational Dental Centre Singapore, 5 Second Hospital Avenue, Singapore 168938, SingaporeDepartment of Biosciences, Universidade Federal de São Paulo (UNIFESP), Santos 11015-220, SP, BrazilThis study aimed to compare different protocols (Protocol 1: P1; Protocol 2: P2; Protocol 3: P3; Protocol 4: P4) for the extraction of spongin-like collagen (SC) from marine sponges. The SEM micrographs demonstrated a fibrillar structure for the extracts from <i>Chondrilla caribensis</i> and the nodular/particulate aggregates for <i>Aplysina fulva</i>. FTIR showed for all samples peaks similar to collagen for both species. For <i>C. caribensis</i>, the extracts obtained using P2, P3, and P4 protocols presented higher values of extraction yield, TPQ, and GAGs. P2 and P4 showed higher values of SC concentration and for antioxidant analysis. For <i>A. fulva</i>, P2, P3, and P4 provided a higher extraction yield besides an increase in the antioxidant assay. For both species, no difference was observed for Col quantification and TPQ analysis; also, higher values of GAGs were found using P2 and P4. Fibroblast proliferation observed for <i>C. caribensis</i> was lower for P1 on day 1 and for P2 and P3 on day 3 (for 50%) compared to the control group. There was a significant reduction in fibroblast cell proliferation for all <i>A. fulva</i> extracts evaluated. It can be concluded that protocols P2 and P4 were more efficient for extracting SC from <i>C. caribensis</i>.https://www.mdpi.com/2077-0375/11/7/522spongin-like collagenextraction protocolsmarine spongesbiomaterials |
spellingShingle | Tiago A. T. Araújo Amanda de Souza Alan F. Santana Anna Rafaela C. Braga Márcio R. Custódio Fábio R. Simões Gabriela M. Araújo Antônio Miranda Flávio Alves Renata N. Granito Na Yu Ana Claudia M. Renno Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (<i>Chondrilla caribensis</i> and <i>Aplysina fulva</i>): Physicochemical Properties and In Vitro Biological Analysis Membranes spongin-like collagen extraction protocols marine sponges biomaterials |
title | Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (<i>Chondrilla caribensis</i> and <i>Aplysina fulva</i>): Physicochemical Properties and In Vitro Biological Analysis |
title_full | Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (<i>Chondrilla caribensis</i> and <i>Aplysina fulva</i>): Physicochemical Properties and In Vitro Biological Analysis |
title_fullStr | Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (<i>Chondrilla caribensis</i> and <i>Aplysina fulva</i>): Physicochemical Properties and In Vitro Biological Analysis |
title_full_unstemmed | Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (<i>Chondrilla caribensis</i> and <i>Aplysina fulva</i>): Physicochemical Properties and In Vitro Biological Analysis |
title_short | Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (<i>Chondrilla caribensis</i> and <i>Aplysina fulva</i>): Physicochemical Properties and In Vitro Biological Analysis |
title_sort | comparison of different methods for spongin like collagen extraction from marine sponges i chondrilla caribensis i and i aplysina fulva i physicochemical properties and in vitro biological analysis |
topic | spongin-like collagen extraction protocols marine sponges biomaterials |
url | https://www.mdpi.com/2077-0375/11/7/522 |
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