PDIA6 contributes to aerobic glycolysis and cancer progression in oral squamous cell carcinoma
Abstract Background/objective Accumulated evidence has demonstrated that aerobic glycolysis serves as a regulator of tumor cell growth, invasion, and angiogenesis. Herein, we explored the role of protein disulfide isomerase family 6 (PDIA6) in the aerobic glycolysis and the progression of oral squam...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-03-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-021-02190-w |
| _version_ | 1818965164826820608 |
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| author | Ling Mao Xiaoweng Wu Zhengpeng Gong Ming Yu Zhi Huang |
| author_facet | Ling Mao Xiaoweng Wu Zhengpeng Gong Ming Yu Zhi Huang |
| author_sort | Ling Mao |
| collection | DOAJ |
| description | Abstract Background/objective Accumulated evidence has demonstrated that aerobic glycolysis serves as a regulator of tumor cell growth, invasion, and angiogenesis. Herein, we explored the role of protein disulfide isomerase family 6 (PDIA6) in the aerobic glycolysis and the progression of oral squamous cell carcinoma (OSCC). Methods The expression pattern of PDIA6 in OSCC tissues was determined by qPCR and western blotting. Lentivirus and small interfering RNAs (siRNAs) were introduced into cells to upregulate and downregulate PDIA6 expression. CCK-8, flow cytometry, transwell, and xenotransplantation models were applied to detect cell proliferation, apoptosis, migration, invasion, and tumorigenesis, respectively. Results A high expression pattern of PDIA6 was observed in OSCC tissues, which was closely associated with lower overall survival and malignant clinical features in OSCC. Compared with the control group, overexpression of PDIA6 induced significant enhancements in cell growth, migration, invasiveness, and tumorigenesis and decreased cell apoptosis, while knockdown of PDIA6 caused opposite results. In addition, overexpression of PDIA6 increased glucose consumption, lactate production, and ATP level in OSCC cells. Conclusion This study demonstrated that PDIA6 expression was elevated in OSCC tissues, and overexpression of it promoted aerobic glycolysis and OSCC progression. |
| first_indexed | 2024-12-20T13:12:40Z |
| format | Article |
| id | doaj.art-ccc2881b1e3541ccb88069541be19b16 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-12-20T13:12:40Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-ccc2881b1e3541ccb88069541be19b162022-12-21T19:39:37ZengBMCWorld Journal of Surgical Oncology1477-78192021-03-011911910.1186/s12957-021-02190-wPDIA6 contributes to aerobic glycolysis and cancer progression in oral squamous cell carcinomaLing Mao0Xiaoweng Wu1Zhengpeng Gong2Ming Yu3Zhi Huang4The Laboratory of Head and Neck Cancer Research, Hospital and School of Stomatology, Guizhou Medical UniversityDepartment of Imaging, Liaocheng People’s HospitalDepartment of Otorhinolaryngology, The Affiliated Hospital of Guizhou Medical UniversityLaryngology and Otology, the Affiliated Baiyun Hospital of Guizhou Medical UniversityDepartment of Interventional Radiology, the Affiliated Cancer Hospital of Guizhou Medical UniversityAbstract Background/objective Accumulated evidence has demonstrated that aerobic glycolysis serves as a regulator of tumor cell growth, invasion, and angiogenesis. Herein, we explored the role of protein disulfide isomerase family 6 (PDIA6) in the aerobic glycolysis and the progression of oral squamous cell carcinoma (OSCC). Methods The expression pattern of PDIA6 in OSCC tissues was determined by qPCR and western blotting. Lentivirus and small interfering RNAs (siRNAs) were introduced into cells to upregulate and downregulate PDIA6 expression. CCK-8, flow cytometry, transwell, and xenotransplantation models were applied to detect cell proliferation, apoptosis, migration, invasion, and tumorigenesis, respectively. Results A high expression pattern of PDIA6 was observed in OSCC tissues, which was closely associated with lower overall survival and malignant clinical features in OSCC. Compared with the control group, overexpression of PDIA6 induced significant enhancements in cell growth, migration, invasiveness, and tumorigenesis and decreased cell apoptosis, while knockdown of PDIA6 caused opposite results. In addition, overexpression of PDIA6 increased glucose consumption, lactate production, and ATP level in OSCC cells. Conclusion This study demonstrated that PDIA6 expression was elevated in OSCC tissues, and overexpression of it promoted aerobic glycolysis and OSCC progression.https://doi.org/10.1186/s12957-021-02190-wPDIA6ProliferationMigrationAerobic glycolysisTumorigenesis |
| spellingShingle | Ling Mao Xiaoweng Wu Zhengpeng Gong Ming Yu Zhi Huang PDIA6 contributes to aerobic glycolysis and cancer progression in oral squamous cell carcinoma World Journal of Surgical Oncology PDIA6 Proliferation Migration Aerobic glycolysis Tumorigenesis |
| title | PDIA6 contributes to aerobic glycolysis and cancer progression in oral squamous cell carcinoma |
| title_full | PDIA6 contributes to aerobic glycolysis and cancer progression in oral squamous cell carcinoma |
| title_fullStr | PDIA6 contributes to aerobic glycolysis and cancer progression in oral squamous cell carcinoma |
| title_full_unstemmed | PDIA6 contributes to aerobic glycolysis and cancer progression in oral squamous cell carcinoma |
| title_short | PDIA6 contributes to aerobic glycolysis and cancer progression in oral squamous cell carcinoma |
| title_sort | pdia6 contributes to aerobic glycolysis and cancer progression in oral squamous cell carcinoma |
| topic | PDIA6 Proliferation Migration Aerobic glycolysis Tumorigenesis |
| url | https://doi.org/10.1186/s12957-021-02190-w |
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