Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and 31P-NMR
The analysis of HDL and LDL is important for the further understanding of atherosclerosis because changes of the protein and lipid moieties occur under pathological conditions. Because destruction of lipids leads to the formation of well-defined products such as lysophospholipids or chlorohydrins, m...
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| Format: | Article |
| Language: | English |
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Elsevier
2001-09-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520341961 |
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| author | Jürgen Schiller Olaf Zschörnig Marijana Petkovic´ Matthias Müller Jürgen Arnhold Klaus Arnold |
| author_facet | Jürgen Schiller Olaf Zschörnig Marijana Petkovic´ Matthias Müller Jürgen Arnhold Klaus Arnold |
| author_sort | Jürgen Schiller |
| collection | DOAJ |
| description | The analysis of HDL and LDL is important for the further understanding of atherosclerosis because changes of the protein and lipid moieties occur under pathological conditions. Because destruction of lipids leads to the formation of well-defined products such as lysophospholipids or chlorohydrins, methods that allow their fast and reliable determination would be useful. In this study, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied for the analysis of the lipid composition of human lipoproteins. These data were compared with high resolution 31P-NMR spectroscopy. Differences between LDL and HDL in sphingomyelin and phosphatidylcholine content could be monitored by NMR and mass spectrometry, and differences with respect to the extraction efficiency were found by MALDI-TOF MS. Additionally, treatment of LDL with hypochlorite and phospholipase A2 resulted in marked changes (formation of chlorohydrines and lysolipids). Lysophosphatidylcholines were detectable by both methods, whereas MALDI-TOF MS failed to detect chlorohydrines of phospholipids. We conclude that MALDI-TOF MS provides rapidly a reliable lipid profile of lipoproteins. However, a previous lipid separation must be performed to detect lipid oxidation products. NMR can be directly applied, but suffers from lower sensitivity, and provides only limited information on fatty acid composition. —Schiller, J., O. Zschörnig, M. Petković, M. Müller, J. Arnhold, and K. Arnold. Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and 31P-NMR. |
| first_indexed | 2024-12-20T05:40:49Z |
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| id | doaj.art-ccc69606e3e94bd9b91ece9eba7103f3 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T05:40:49Z |
| publishDate | 2001-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-ccc69606e3e94bd9b91ece9eba7103f32022-12-21T19:51:28ZengElsevierJournal of Lipid Research0022-22752001-09-0142915011508Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and 31P-NMRJürgen Schiller0Olaf Zschörnig1Marijana Petkovic´2Matthias Müller3Jürgen Arnhold4Klaus Arnold5To whom correspondence should be addressed. e-mail:; Institute of Medical Physics and Biophysics, Medical Department, University of Leipzig, Liebigstr. 27, 04103 Leipzig, GermanyInstitute of Medical Physics and Biophysics, Medical Department, University of Leipzig, Liebigstr. 27, 04103 Leipzig, GermanyInstitute of Medical Physics and Biophysics, Medical Department, University of Leipzig, Liebigstr. 27, 04103 Leipzig, GermanyInstitute of Medical Physics and Biophysics, Medical Department, University of Leipzig, Liebigstr. 27, 04103 Leipzig, GermanyInstitute of Medical Physics and Biophysics, Medical Department, University of Leipzig, Liebigstr. 27, 04103 Leipzig, GermanyInstitute of Medical Physics and Biophysics, Medical Department, University of Leipzig, Liebigstr. 27, 04103 Leipzig, GermanyThe analysis of HDL and LDL is important for the further understanding of atherosclerosis because changes of the protein and lipid moieties occur under pathological conditions. Because destruction of lipids leads to the formation of well-defined products such as lysophospholipids or chlorohydrins, methods that allow their fast and reliable determination would be useful. In this study, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied for the analysis of the lipid composition of human lipoproteins. These data were compared with high resolution 31P-NMR spectroscopy. Differences between LDL and HDL in sphingomyelin and phosphatidylcholine content could be monitored by NMR and mass spectrometry, and differences with respect to the extraction efficiency were found by MALDI-TOF MS. Additionally, treatment of LDL with hypochlorite and phospholipase A2 resulted in marked changes (formation of chlorohydrines and lysolipids). Lysophosphatidylcholines were detectable by both methods, whereas MALDI-TOF MS failed to detect chlorohydrines of phospholipids. We conclude that MALDI-TOF MS provides rapidly a reliable lipid profile of lipoproteins. However, a previous lipid separation must be performed to detect lipid oxidation products. NMR can be directly applied, but suffers from lower sensitivity, and provides only limited information on fatty acid composition. —Schiller, J., O. Zschörnig, M. Petković, M. Müller, J. Arnhold, and K. Arnold. Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and 31P-NMR.http://www.sciencedirect.com/science/article/pii/S0022227520341961MALDI-TOF MSlysolipidschlorohydrine |
| spellingShingle | Jürgen Schiller Olaf Zschörnig Marijana Petkovic´ Matthias Müller Jürgen Arnhold Klaus Arnold Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and 31P-NMR Journal of Lipid Research MALDI-TOF MS lysolipids chlorohydrine |
| title | Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and 31P-NMR |
| title_full | Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and 31P-NMR |
| title_fullStr | Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and 31P-NMR |
| title_full_unstemmed | Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and 31P-NMR |
| title_short | Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and 31P-NMR |
| title_sort | lipid analysis of human hdl and ldl by maldi tof mass spectrometry and 31p nmr |
| topic | MALDI-TOF MS lysolipids chlorohydrine |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520341961 |
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