Hydroethanolic Extract of <i>Urtica dioica</i> L. (Stinging Nettle) Leaves as Disaccharidase Inhibitor and Glucose Transport in Caco-2 Hinderer
Herbal treatment for diabetes mellitus is widely used. The pharmacological activity is thought to be due to the phenolic compounds found in the plant leaves. The present study aims to investigate the phytochemical composition of <i>Urtica dioica</i> (UD) hydroethanolic extract and to scr...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/24/8872 |
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| author | Mohammad A. Altamimi Ibrahim M. Abu-Reidah Almothana Altamimi Nidal Jaradat |
| author_facet | Mohammad A. Altamimi Ibrahim M. Abu-Reidah Almothana Altamimi Nidal Jaradat |
| author_sort | Mohammad A. Altamimi |
| collection | DOAJ |
| description | Herbal treatment for diabetes mellitus is widely used. The pharmacological activity is thought to be due to the phenolic compounds found in the plant leaves. The present study aims to investigate the phytochemical composition of <i>Urtica dioica</i> (UD) hydroethanolic extract and to screen its antidiabetic activity by disaccharidase hindering and glucose transport in Caco-2 cells. The results have shown that a total of 13 phenolic compounds in this work, viz. caffeic and coumaric acid esters (<b>1</b>, <b>2</b>, <b>4</b>–<b>7</b>, <b>10</b>), ferulic derivative (<b>3</b>), and flavonoid glycosides (<b>8</b>, <b>9</b>, <b>11</b>–<b>13</b>), were identified using HPLC-DAD-ESI/MS<sup>2</sup>. The most abundant phenolic compounds were <b>8</b> (rutin) followed by <b>6</b> (caffeoylquinic acid III). Less predominant compounds were <b>4</b> (caffeoylquinic acid II) and <b>11</b> (kaempferol-O-rutinoside). The UD hydroethanolic extract showed 56%, 45%, and 28% (1.0 mg/mL) inhibition level for maltase, sucrase, and lactase, respectively. On the other hand, glucose transport was 1.48 times less at 1.0 mg/mL UD extract compared with the control containing no UD extract. The results confirmed that <i>U. dioica</i> is a potential antidiabetic herb having both anti-disaccharidase and glucose transport inhibitory properties, which explained the use of UD in traditional medicine. |
| first_indexed | 2024-03-09T16:02:37Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T16:02:37Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ccc9c170024546ef8336dbd9a44141eb2023-11-24T16:58:53ZengMDPI AGMolecules1420-30492022-12-012724887210.3390/molecules27248872Hydroethanolic Extract of <i>Urtica dioica</i> L. (Stinging Nettle) Leaves as Disaccharidase Inhibitor and Glucose Transport in Caco-2 HindererMohammad A. Altamimi0Ibrahim M. Abu-Reidah1Almothana Altamimi2Nidal Jaradat3Department of Nutrition and Food Technology, An-Najah National University, Nablus P.O. Box 7, PalestineDepartment of Industrial Chemistry, Faculty of Science, Arab American University, Jenin P.O. Box 240, PalestineDepartment of Medicine and Surgery, Università di Napoli Federico II, 80131 Naples, ItalyDepartment of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus P.O. Box 7, PalestineHerbal treatment for diabetes mellitus is widely used. The pharmacological activity is thought to be due to the phenolic compounds found in the plant leaves. The present study aims to investigate the phytochemical composition of <i>Urtica dioica</i> (UD) hydroethanolic extract and to screen its antidiabetic activity by disaccharidase hindering and glucose transport in Caco-2 cells. The results have shown that a total of 13 phenolic compounds in this work, viz. caffeic and coumaric acid esters (<b>1</b>, <b>2</b>, <b>4</b>–<b>7</b>, <b>10</b>), ferulic derivative (<b>3</b>), and flavonoid glycosides (<b>8</b>, <b>9</b>, <b>11</b>–<b>13</b>), were identified using HPLC-DAD-ESI/MS<sup>2</sup>. The most abundant phenolic compounds were <b>8</b> (rutin) followed by <b>6</b> (caffeoylquinic acid III). Less predominant compounds were <b>4</b> (caffeoylquinic acid II) and <b>11</b> (kaempferol-O-rutinoside). The UD hydroethanolic extract showed 56%, 45%, and 28% (1.0 mg/mL) inhibition level for maltase, sucrase, and lactase, respectively. On the other hand, glucose transport was 1.48 times less at 1.0 mg/mL UD extract compared with the control containing no UD extract. The results confirmed that <i>U. dioica</i> is a potential antidiabetic herb having both anti-disaccharidase and glucose transport inhibitory properties, which explained the use of UD in traditional medicine.https://www.mdpi.com/1420-3049/27/24/8872<i>Urtica dioica</i>diabetes mellitusmedicinal plantsCaco-2α-glucosidaseglucose transport |
| spellingShingle | Mohammad A. Altamimi Ibrahim M. Abu-Reidah Almothana Altamimi Nidal Jaradat Hydroethanolic Extract of <i>Urtica dioica</i> L. (Stinging Nettle) Leaves as Disaccharidase Inhibitor and Glucose Transport in Caco-2 Hinderer Molecules <i>Urtica dioica</i> diabetes mellitus medicinal plants Caco-2 α-glucosidase glucose transport |
| title | Hydroethanolic Extract of <i>Urtica dioica</i> L. (Stinging Nettle) Leaves as Disaccharidase Inhibitor and Glucose Transport in Caco-2 Hinderer |
| title_full | Hydroethanolic Extract of <i>Urtica dioica</i> L. (Stinging Nettle) Leaves as Disaccharidase Inhibitor and Glucose Transport in Caco-2 Hinderer |
| title_fullStr | Hydroethanolic Extract of <i>Urtica dioica</i> L. (Stinging Nettle) Leaves as Disaccharidase Inhibitor and Glucose Transport in Caco-2 Hinderer |
| title_full_unstemmed | Hydroethanolic Extract of <i>Urtica dioica</i> L. (Stinging Nettle) Leaves as Disaccharidase Inhibitor and Glucose Transport in Caco-2 Hinderer |
| title_short | Hydroethanolic Extract of <i>Urtica dioica</i> L. (Stinging Nettle) Leaves as Disaccharidase Inhibitor and Glucose Transport in Caco-2 Hinderer |
| title_sort | hydroethanolic extract of i urtica dioica i l stinging nettle leaves as disaccharidase inhibitor and glucose transport in caco 2 hinderer |
| topic | <i>Urtica dioica</i> diabetes mellitus medicinal plants Caco-2 α-glucosidase glucose transport |
| url | https://www.mdpi.com/1420-3049/27/24/8872 |
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