Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p
Abstract Pseudogene-derived transcripts, especially those barely transcribed in normal tissues, have been regarded as a kind of non-coding RNAs, and present potential functions in tumorigenicity and tumor development in human beings. However, their exact effects on hepatocellular carcinoma (HCC) rem...
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24597-5 |
| _version_ | 1797977565172858880 |
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| author | Nan Wang Simin Guo Fengjie Hao Yifan Zhang Yongjun Chen Xiaochun Fei Junqing Wang |
| author_facet | Nan Wang Simin Guo Fengjie Hao Yifan Zhang Yongjun Chen Xiaochun Fei Junqing Wang |
| author_sort | Nan Wang |
| collection | DOAJ |
| description | Abstract Pseudogene-derived transcripts, especially those barely transcribed in normal tissues, have been regarded as a kind of non-coding RNAs, and present potential functions in tumorigenicity and tumor development in human beings. However, their exact effects on hepatocellular carcinoma (HCC) remain largely unknown. On basis of our previous research and the constructed online database for the non-coding RNAs related to HCC, a series of pseudogene transcripts have been discovered, and SNRPFP1, the homologous pseudogene of SNRPF, was found to produce an anomalously high expression long non-coding RNA in HCC. In this study, we validated the expression of the SNRPFP1 transcript in both HCC tissues and cell lines. The adverse correlation between SNRPFP1 expression and patients’ outcomes was observed. And depletion of SNRPF1 in HCC cells significantly suppressed cell proliferation and apoptosis resistance. Meanwhile, the motility of HCC cells was potently impaired. Interestingly, miR-126-5p, one of the tumor-suppressive genes commonly decreased in HCC, was found negatively expressed and correlated with SNRPF1, and a specific region of SNRPF1 transcript is directly binding to miR-126-5p in a molecular sponge way. The rescue experiment by knock-out miR-126-5p significantly reversed the cell growth suppression and a higher ratio of cell apoptosis induced by SNRPF1 depletion. Lastly, we concluded that SNRPF1 is a pseudogene active in HCC, and its abnormally over-expressed transcript is a strong promoter of HCC cell progress in vitro by sponging miR-126-5p. We believe that the findings in this study provide new strategies for HCC prevention and therapeutic treatment. |
| first_indexed | 2024-04-11T05:08:58Z |
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| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T05:08:58Z |
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| spelling | doaj.art-cccbc38183e74aaaae76ecfda3aa59062022-12-25T12:11:19ZengNature PortfolioScientific Reports2045-23222022-12-0112111310.1038/s41598-022-24597-5Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5pNan Wang0Simin Guo1Fengjie Hao2Yifan Zhang3Yongjun Chen4Xiaochun Fei5Junqing Wang6Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Infectious Disease, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Pathology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineAbstract Pseudogene-derived transcripts, especially those barely transcribed in normal tissues, have been regarded as a kind of non-coding RNAs, and present potential functions in tumorigenicity and tumor development in human beings. However, their exact effects on hepatocellular carcinoma (HCC) remain largely unknown. On basis of our previous research and the constructed online database for the non-coding RNAs related to HCC, a series of pseudogene transcripts have been discovered, and SNRPFP1, the homologous pseudogene of SNRPF, was found to produce an anomalously high expression long non-coding RNA in HCC. In this study, we validated the expression of the SNRPFP1 transcript in both HCC tissues and cell lines. The adverse correlation between SNRPFP1 expression and patients’ outcomes was observed. And depletion of SNRPF1 in HCC cells significantly suppressed cell proliferation and apoptosis resistance. Meanwhile, the motility of HCC cells was potently impaired. Interestingly, miR-126-5p, one of the tumor-suppressive genes commonly decreased in HCC, was found negatively expressed and correlated with SNRPF1, and a specific region of SNRPF1 transcript is directly binding to miR-126-5p in a molecular sponge way. The rescue experiment by knock-out miR-126-5p significantly reversed the cell growth suppression and a higher ratio of cell apoptosis induced by SNRPF1 depletion. Lastly, we concluded that SNRPF1 is a pseudogene active in HCC, and its abnormally over-expressed transcript is a strong promoter of HCC cell progress in vitro by sponging miR-126-5p. We believe that the findings in this study provide new strategies for HCC prevention and therapeutic treatment.https://doi.org/10.1038/s41598-022-24597-5 |
| spellingShingle | Nan Wang Simin Guo Fengjie Hao Yifan Zhang Yongjun Chen Xiaochun Fei Junqing Wang Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p Scientific Reports |
| title | Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p |
| title_full | Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p |
| title_fullStr | Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p |
| title_full_unstemmed | Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p |
| title_short | Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p |
| title_sort | pseudogene snrpfp1 derived long non coding rna facilitates hepatocellular carcinoma progress in vitro by sponging tumor suppressive mir 126 5p |
| url | https://doi.org/10.1038/s41598-022-24597-5 |
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