Administration of probiotics affects Artemia franciscana metanauplii intestinal ultrastructure and offers resistance against a Photobacterium damselae ssp. piscicida induced oxidative stress response
The effects of Photobacterium damselae ssp. piscicida (Phdp) on immune responses and intestinal ultrastructure of Artemia franciscana following infection and their amelioration by the probiotic bacteria Bacillus subtilis, Lactobacillus plantarum and Lactococcus lactis were evaluated. Pathogen growth...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Fish and Shellfish Immunology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667011923000336 |
| _version_ | 1827593535712395264 |
|---|---|
| author | Maria Touraki Anna Chanou Vasiliki Mavridou Vasiliki Tsertseli Maria Tsiridi Emmanuel Panteris |
| author_facet | Maria Touraki Anna Chanou Vasiliki Mavridou Vasiliki Tsertseli Maria Tsiridi Emmanuel Panteris |
| author_sort | Maria Touraki |
| collection | DOAJ |
| description | The effects of Photobacterium damselae ssp. piscicida (Phdp) on immune responses and intestinal ultrastructure of Artemia franciscana following infection and their amelioration by the probiotic bacteria Bacillus subtilis, Lactobacillus plantarum and Lactococcus lactis were evaluated. Pathogen growth inhibition in coculture with each probiotic and its virulence against Artemia were confirmed with an LC50 of 105 CFU mL−1. Phdp administration to Artemia at sublethal levels resulted in depletion of superoxide dismutase, glutathione reductase, glutathione transferase and phenoloxidase activities, extensive lipid peroxidation and reduced survival. Following a combined administration of each probiotic and the pathogen, enzyme activities and survival were significantly higher, while lipid peroxidation was reduced, compared to the infected group with no probiotic treatment (P < 0.05). The transmission electron microscopy study revealed that pathogen infection resulted in disarranged and fragmented microvilli, formation of empty or pathogen containing cytoplasmic vacuoles and damaged mitochondria. In the probiotic-treated and Phdp-infected series, intestinal cells showed normal appearance, except for the presence of pathogen-containing vacuoles and highly ordered but laterally stacked microvilli. The results of the present study indicate that Phdp induces cell death through an oxidative stress response and probiotics enhance Artemia immune responses to protect it against the Phdp induced damage. |
| first_indexed | 2024-03-09T02:13:06Z |
| format | Article |
| id | doaj.art-cccbe13be99042b3be998690c1d45ea7 |
| institution | Directory Open Access Journal |
| issn | 2667-0119 |
| language | English |
| last_indexed | 2024-03-09T02:13:06Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Fish and Shellfish Immunology Reports |
| spelling | doaj.art-cccbe13be99042b3be998690c1d45ea72023-12-07T05:31:16ZengElsevierFish and Shellfish Immunology Reports2667-01192023-12-015100113Administration of probiotics affects Artemia franciscana metanauplii intestinal ultrastructure and offers resistance against a Photobacterium damselae ssp. piscicida induced oxidative stress responseMaria Touraki0Anna Chanou1Vasiliki Mavridou2Vasiliki Tsertseli3Maria Tsiridi4Emmanuel Panteris5Laboratory of General Biology, Division of Genetics, Development and Molecular Biology, Department of Biology, School of Sciences, Aristotle University of Thessaloniki (A.U.TH.), Thessaloniki 54 124, Greece; Corresponding author.Laboratory of General Biology, Division of Genetics, Development and Molecular Biology, Department of Biology, School of Sciences, Aristotle University of Thessaloniki (A.U.TH.), Thessaloniki 54 124, GreeceLaboratory of General Biology, Division of Genetics, Development and Molecular Biology, Department of Biology, School of Sciences, Aristotle University of Thessaloniki (A.U.TH.), Thessaloniki 54 124, GreeceLaboratory of General Biology, Division of Genetics, Development and Molecular Biology, Department of Biology, School of Sciences, Aristotle University of Thessaloniki (A.U.TH.), Thessaloniki 54 124, GreeceLaboratory of General Biology, Division of Genetics, Development and Molecular Biology, Department of Biology, School of Sciences, Aristotle University of Thessaloniki (A.U.TH.), Thessaloniki 54 124, GreeceDepartment of Botany, School of Biology, Aristotle University of Thessaloniki, Thessaloniki 54124, GreeceThe effects of Photobacterium damselae ssp. piscicida (Phdp) on immune responses and intestinal ultrastructure of Artemia franciscana following infection and their amelioration by the probiotic bacteria Bacillus subtilis, Lactobacillus plantarum and Lactococcus lactis were evaluated. Pathogen growth inhibition in coculture with each probiotic and its virulence against Artemia were confirmed with an LC50 of 105 CFU mL−1. Phdp administration to Artemia at sublethal levels resulted in depletion of superoxide dismutase, glutathione reductase, glutathione transferase and phenoloxidase activities, extensive lipid peroxidation and reduced survival. Following a combined administration of each probiotic and the pathogen, enzyme activities and survival were significantly higher, while lipid peroxidation was reduced, compared to the infected group with no probiotic treatment (P < 0.05). The transmission electron microscopy study revealed that pathogen infection resulted in disarranged and fragmented microvilli, formation of empty or pathogen containing cytoplasmic vacuoles and damaged mitochondria. In the probiotic-treated and Phdp-infected series, intestinal cells showed normal appearance, except for the presence of pathogen-containing vacuoles and highly ordered but laterally stacked microvilli. The results of the present study indicate that Phdp induces cell death through an oxidative stress response and probiotics enhance Artemia immune responses to protect it against the Phdp induced damage.http://www.sciencedirect.com/science/article/pii/S2667011923000336Free vibrationSpatially closed-coupled plates with curved edgesArbitrary connection and boundary conditionsVirtual spring stiffness functionsJacobian differential quadrature methodHammer modal test |
| spellingShingle | Maria Touraki Anna Chanou Vasiliki Mavridou Vasiliki Tsertseli Maria Tsiridi Emmanuel Panteris Administration of probiotics affects Artemia franciscana metanauplii intestinal ultrastructure and offers resistance against a Photobacterium damselae ssp. piscicida induced oxidative stress response Fish and Shellfish Immunology Reports Free vibration Spatially closed-coupled plates with curved edges Arbitrary connection and boundary conditions Virtual spring stiffness functions Jacobian differential quadrature method Hammer modal test |
| title | Administration of probiotics affects Artemia franciscana metanauplii intestinal ultrastructure and offers resistance against a Photobacterium damselae ssp. piscicida induced oxidative stress response |
| title_full | Administration of probiotics affects Artemia franciscana metanauplii intestinal ultrastructure and offers resistance against a Photobacterium damselae ssp. piscicida induced oxidative stress response |
| title_fullStr | Administration of probiotics affects Artemia franciscana metanauplii intestinal ultrastructure and offers resistance against a Photobacterium damselae ssp. piscicida induced oxidative stress response |
| title_full_unstemmed | Administration of probiotics affects Artemia franciscana metanauplii intestinal ultrastructure and offers resistance against a Photobacterium damselae ssp. piscicida induced oxidative stress response |
| title_short | Administration of probiotics affects Artemia franciscana metanauplii intestinal ultrastructure and offers resistance against a Photobacterium damselae ssp. piscicida induced oxidative stress response |
| title_sort | administration of probiotics affects artemia franciscana metanauplii intestinal ultrastructure and offers resistance against a photobacterium damselae ssp piscicida induced oxidative stress response |
| topic | Free vibration Spatially closed-coupled plates with curved edges Arbitrary connection and boundary conditions Virtual spring stiffness functions Jacobian differential quadrature method Hammer modal test |
| url | http://www.sciencedirect.com/science/article/pii/S2667011923000336 |
| work_keys_str_mv | AT mariatouraki administrationofprobioticsaffectsartemiafranciscanametanaupliiintestinalultrastructureandoffersresistanceagainstaphotobacteriumdamselaessppiscicidainducedoxidativestressresponse AT annachanou administrationofprobioticsaffectsartemiafranciscanametanaupliiintestinalultrastructureandoffersresistanceagainstaphotobacteriumdamselaessppiscicidainducedoxidativestressresponse AT vasilikimavridou administrationofprobioticsaffectsartemiafranciscanametanaupliiintestinalultrastructureandoffersresistanceagainstaphotobacteriumdamselaessppiscicidainducedoxidativestressresponse AT vasilikitsertseli administrationofprobioticsaffectsartemiafranciscanametanaupliiintestinalultrastructureandoffersresistanceagainstaphotobacteriumdamselaessppiscicidainducedoxidativestressresponse AT mariatsiridi administrationofprobioticsaffectsartemiafranciscanametanaupliiintestinalultrastructureandoffersresistanceagainstaphotobacteriumdamselaessppiscicidainducedoxidativestressresponse AT emmanuelpanteris administrationofprobioticsaffectsartemiafranciscanametanaupliiintestinalultrastructureandoffersresistanceagainstaphotobacteriumdamselaessppiscicidainducedoxidativestressresponse |