Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats
This study aimed at investigating the effects of intraperitoneal (IP) administration of magnesium sulfate (MgSO<sub>4</sub>) on testicular ischemia-reperfusion (IR) injury in rats. In total, 50 adult Wistar rats were randomly divided into 5 groups. Group 1 received no injection (control)...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Razi Vaccine and Serum Research Institute
2020-03-01
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| Series: | Archives of Razi Institute |
| Subjects: | |
| Online Access: | https://archrazi.areeo.ac.ir/article_120982_a89484bed2625f9cce5b58ea563834b4.pdf |
| Summary: | This study aimed at investigating the effects of intraperitoneal (IP) administration of magnesium sulfate (MgSO<sub>4</sub>) on testicular ischemia-reperfusion (IR) injury in rats. In total, 50 adult Wistar rats were randomly divided into 5 groups. Group 1 received no injection (control); however, group 2 was subjected to 2 h of I and 24 h of R. Subsequently, group 3 was subjected to 2 h of 1, and after 1 h of I, 125 mg/kg MgSO<sub>4</sub> was injected intraperitoneally followed by 24 h of R. Groups 4 and 5 were subjected to the same process as group 3, whereas the rats were injected with 250 and 500 mg/kg of MgSO<sub>4</sub>, respectively. After 24 h, the left testes of all rats were removed for histological analysis and antioxidant activities. According to the results, there was a significant increase in tissue malondialdehyde (MDA) among I/R rats (P<0.05), whereas MgSO<sub>4</sub> decreased I/R-induced MDA (P<0.05). Furthermore, experimental I/R diminished glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels significantly (P<0.05). Moreover, MgSO<sub>4</sub> (250 and 500 mg/kg) increased GPx and SOD activity significantly in I/R rats (P<0.05). Furthermore, seminiferous tubules degenerated, and few spermatocytes were observed in the testis tubules of the I/R rats. Regarding pathological parameters, seminiferous tubules and spermatocyte were normal in the testes of MgSO<sub>4</sub> (250 and 500 mg/kg)-treated experimental I/R-induced rats. In conclusion, this study demonstrated the beneficial effects of MgSO<sub>4</sub> on testicular IR injury in rats. |
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| ISSN: | 0365-3439 2008-9872 |