Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats
This study aimed at investigating the effects of intraperitoneal (IP) administration of magnesium sulfate (MgSO<sub>4</sub>) on testicular ischemia-reperfusion (IR) injury in rats. In total, 50 adult Wistar rats were randomly divided into 5 groups. Group 1 received no injection (control)...
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Razi Vaccine and Serum Research Institute
2020-03-01
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| Series: | Archives of Razi Institute |
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| Online Access: | https://archrazi.areeo.ac.ir/article_120982_a89484bed2625f9cce5b58ea563834b4.pdf |
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| author | S. Moshkelani A. Asghari A. Abedi A. Jahandideh P. Mortazavi |
| author_facet | S. Moshkelani A. Asghari A. Abedi A. Jahandideh P. Mortazavi |
| author_sort | S. Moshkelani |
| collection | DOAJ |
| description | This study aimed at investigating the effects of intraperitoneal (IP) administration of magnesium sulfate (MgSO<sub>4</sub>) on testicular ischemia-reperfusion (IR) injury in rats. In total, 50 adult Wistar rats were randomly divided into 5 groups. Group 1 received no injection (control); however, group 2 was subjected to 2 h of I and 24 h of R. Subsequently, group 3 was subjected to 2 h of 1, and after 1 h of I, 125 mg/kg MgSO<sub>4</sub> was injected intraperitoneally followed by 24 h of R. Groups 4 and 5 were subjected to the same process as group 3, whereas the rats were injected with 250 and 500 mg/kg of MgSO<sub>4</sub>, respectively. After 24 h, the left testes of all rats were removed for histological analysis and antioxidant activities. According to the results, there was a significant increase in tissue malondialdehyde (MDA) among I/R rats (P<0.05), whereas MgSO<sub>4</sub> decreased I/R-induced MDA (P<0.05). Furthermore, experimental I/R diminished glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels significantly (P<0.05). Moreover, MgSO<sub>4</sub> (250 and 500 mg/kg) increased GPx and SOD activity significantly in I/R rats (P<0.05). Furthermore, seminiferous tubules degenerated, and few spermatocytes were observed in the testis tubules of the I/R rats. Regarding pathological parameters, seminiferous tubules and spermatocyte were normal in the testes of MgSO<sub>4</sub> (250 and 500 mg/kg)-treated experimental I/R-induced rats. In conclusion, this study demonstrated the beneficial effects of MgSO<sub>4</sub> on testicular IR injury in rats. |
| first_indexed | 2024-12-11T16:53:54Z |
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| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-11T16:53:54Z |
| publishDate | 2020-03-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-ccccdb3ed3de45589746c86fd6c01e282022-12-22T00:58:01ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722020-03-01751839110.22092/ari.2018.123458.1251120982Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in RatsS. Moshkelani0A. Asghari1A. Abedi2A. Jahandideh3P. Mortazavi4Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, IranThis study aimed at investigating the effects of intraperitoneal (IP) administration of magnesium sulfate (MgSO<sub>4</sub>) on testicular ischemia-reperfusion (IR) injury in rats. In total, 50 adult Wistar rats were randomly divided into 5 groups. Group 1 received no injection (control); however, group 2 was subjected to 2 h of I and 24 h of R. Subsequently, group 3 was subjected to 2 h of 1, and after 1 h of I, 125 mg/kg MgSO<sub>4</sub> was injected intraperitoneally followed by 24 h of R. Groups 4 and 5 were subjected to the same process as group 3, whereas the rats were injected with 250 and 500 mg/kg of MgSO<sub>4</sub>, respectively. After 24 h, the left testes of all rats were removed for histological analysis and antioxidant activities. According to the results, there was a significant increase in tissue malondialdehyde (MDA) among I/R rats (P<0.05), whereas MgSO<sub>4</sub> decreased I/R-induced MDA (P<0.05). Furthermore, experimental I/R diminished glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels significantly (P<0.05). Moreover, MgSO<sub>4</sub> (250 and 500 mg/kg) increased GPx and SOD activity significantly in I/R rats (P<0.05). Furthermore, seminiferous tubules degenerated, and few spermatocytes were observed in the testis tubules of the I/R rats. Regarding pathological parameters, seminiferous tubules and spermatocyte were normal in the testes of MgSO<sub>4</sub> (250 and 500 mg/kg)-treated experimental I/R-induced rats. In conclusion, this study demonstrated the beneficial effects of MgSO<sub>4</sub> on testicular IR injury in rats.https://archrazi.areeo.ac.ir/article_120982_a89484bed2625f9cce5b58ea563834b4.pdfischemia/reperfusion (i/r)magnesium sulfaterattestis |
| spellingShingle | S. Moshkelani A. Asghari A. Abedi A. Jahandideh P. Mortazavi Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats Archives of Razi Institute ischemia/reperfusion (i/r) magnesium sulfate rat testis |
| title | Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats |
| title_full | Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats |
| title_fullStr | Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats |
| title_full_unstemmed | Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats |
| title_short | Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats |
| title_sort | effects of magnesium sulfate administration on testicular ischemia reperfusion injury in rats |
| topic | ischemia/reperfusion (i/r) magnesium sulfate rat testis |
| url | https://archrazi.areeo.ac.ir/article_120982_a89484bed2625f9cce5b58ea563834b4.pdf |
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