Genetic diversity analysis based on molecular marker and quantitative traits of the response of different tomato (Lycopersicon esculentum Mill.) cultivars to drought stress
The drought tolerance of tomato (Lycopersicon esculentum Mill.) is a trait needing urgent improvement due to recent climate changes and limited water availability. We therefore conducted a greenhouse screening experiment to identify tomato cultivars with improved drought tolerance. Several...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641500126M.pdf |
| Summary: | The drought tolerance of tomato (Lycopersicon esculentum Mill.) is a trait
needing urgent improvement due to recent climate changes and limited water
availability. We therefore conducted a greenhouse screening experiment to
identify tomato cultivars with improved drought tolerance. Several
sensitivity and tolerance indices were computed based on morphological
markers. With the aim of establishing a correlation to these markers, a total
of 16 inter-simple sequence repeat (ISSR) primers were used, the genetic
diversity among cultivars was elucidated and clustering the cultivars into
groups based on their molecular profiles was performed. The obtained results
indicated that selection indices, such as geometric mean productivity (GMP),
mean productivity (MP), tolerance index (TOL),and stress tolerance index
(STI), represented suitable indices for screening the drought tolerance of
tomato cultivars. An interesting correlation of the ISSR analyses to these
morphological findings was established according to 83 detectable fragments
derived from 10 primers. The highest value of the effective multiplex ratio
(EMR) and marker index (MI) was detected for primer INC7 followed by INC1.
Based on Jaccard's similarity coefficients, the genetic distance of the
genotypes varied from 0.702 to 0.942 with a mean value of 0.882. The results
showed a clear-cut separation of the 15 tomato cultivars due to their genetic
variability, making them a valuable genetic source for their incorporation
into potential breeding programs. Molecular data were in good agreement with
the results as regards selection indices, and both of them will be useful
tools for improvement of the tomato germplasm. |
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| ISSN: | 0354-4664 1821-4339 |