Molecular characterization and expression analysis of pitaya (Hylocereus polyrhizus) HpLRR genes in response to Neoscytalidium dimidiatum infection
Abstract Background Canker disease caused by Neoscytalidium dimidiatum is a devastating disease resulting in a major loss to the pitaya industry. However, resistance proteins in plants play crucial roles to against pathogen infection. Among resistance proteins, the leucine-rich repeat (LRR) protein...
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02368-6 |
| _version_ | 1818158333993943040 |
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| author | Min Xu Cheng-Li Liu Yu Fu Zhi-Wen Liao Pan-Yang Guo Rui Xiong Yu Cheng Shuang-Shuang Wei Jia-Quan Huang Hua Tang |
| author_facet | Min Xu Cheng-Li Liu Yu Fu Zhi-Wen Liao Pan-Yang Guo Rui Xiong Yu Cheng Shuang-Shuang Wei Jia-Quan Huang Hua Tang |
| author_sort | Min Xu |
| collection | DOAJ |
| description | Abstract Background Canker disease caused by Neoscytalidium dimidiatum is a devastating disease resulting in a major loss to the pitaya industry. However, resistance proteins in plants play crucial roles to against pathogen infection. Among resistance proteins, the leucine-rich repeat (LRR) protein is a major family that plays crucial roles in plant growth, development, and biotic and abiotic stress responses, especially in disease defense. Results In the present study, a transcriptomics analysis identified a total of 272 LRR genes, 233 of which had coding sequences (CDSs), in the plant pitaya (Hylocereus polyrhizus) in response to fungal Neoscytalidium dimidiatum infection. These genes were divided into various subgroups based on specific domains and phylogenetic analysis. Molecular characterization, functional annotation of proteins, and an expression analysis of the LRR genes were conducted. Additionally, four LRR genes (CL445.Contig4_All, Unigene28_All, CL28.Contig2_All, and Unigene2712_All, which were selected because they had the four longest CDSs were further assessed using quantitative reverse transcription PCR (qRT-PCR) at different fungal infection stages in different pitaya species (Hylocereus polyrhizus and Hylocereus undatus), in different pitaya tissues, and after treatment with salicylic acid (SA), methyl jasmonate (MeJA), and abscisic acid (ABA) hormones. The associated protein functions and roles in signaling pathways were identified. Conclusions This study provides a comprehensive overview of the HpLRR family genes at transcriptional level in pitaya in response to N. dimidiatum infection, it will be helpful to understand the molecular mechanism of pitaya canker disease, and lay a strong foundation for further research. |
| first_indexed | 2024-12-11T15:28:26Z |
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| id | doaj.art-cccf951873744b0f8cddb241d4c87a94 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-11T15:28:26Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-cccf951873744b0f8cddb241d4c87a942022-12-22T01:00:08ZengBMCBMC Plant Biology1471-22292020-04-0120112010.1186/s12870-020-02368-6Molecular characterization and expression analysis of pitaya (Hylocereus polyrhizus) HpLRR genes in response to Neoscytalidium dimidiatum infectionMin Xu0Cheng-Li Liu1Yu Fu2Zhi-Wen Liao3Pan-Yang Guo4Rui Xiong5Yu Cheng6Shuang-Shuang Wei7Jia-Quan Huang8Hua Tang9Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan UniversityAbstract Background Canker disease caused by Neoscytalidium dimidiatum is a devastating disease resulting in a major loss to the pitaya industry. However, resistance proteins in plants play crucial roles to against pathogen infection. Among resistance proteins, the leucine-rich repeat (LRR) protein is a major family that plays crucial roles in plant growth, development, and biotic and abiotic stress responses, especially in disease defense. Results In the present study, a transcriptomics analysis identified a total of 272 LRR genes, 233 of which had coding sequences (CDSs), in the plant pitaya (Hylocereus polyrhizus) in response to fungal Neoscytalidium dimidiatum infection. These genes were divided into various subgroups based on specific domains and phylogenetic analysis. Molecular characterization, functional annotation of proteins, and an expression analysis of the LRR genes were conducted. Additionally, four LRR genes (CL445.Contig4_All, Unigene28_All, CL28.Contig2_All, and Unigene2712_All, which were selected because they had the four longest CDSs were further assessed using quantitative reverse transcription PCR (qRT-PCR) at different fungal infection stages in different pitaya species (Hylocereus polyrhizus and Hylocereus undatus), in different pitaya tissues, and after treatment with salicylic acid (SA), methyl jasmonate (MeJA), and abscisic acid (ABA) hormones. The associated protein functions and roles in signaling pathways were identified. Conclusions This study provides a comprehensive overview of the HpLRR family genes at transcriptional level in pitaya in response to N. dimidiatum infection, it will be helpful to understand the molecular mechanism of pitaya canker disease, and lay a strong foundation for further research.http://link.springer.com/article/10.1186/s12870-020-02368-6PitayaCanker diseaseNeoscytalidium dimidiatumTranscriptomicsLeucine-rich-repeat genesExpression analysis |
| spellingShingle | Min Xu Cheng-Li Liu Yu Fu Zhi-Wen Liao Pan-Yang Guo Rui Xiong Yu Cheng Shuang-Shuang Wei Jia-Quan Huang Hua Tang Molecular characterization and expression analysis of pitaya (Hylocereus polyrhizus) HpLRR genes in response to Neoscytalidium dimidiatum infection BMC Plant Biology Pitaya Canker disease Neoscytalidium dimidiatum Transcriptomics Leucine-rich-repeat genes Expression analysis |
| title | Molecular characterization and expression analysis of pitaya (Hylocereus polyrhizus) HpLRR genes in response to Neoscytalidium dimidiatum infection |
| title_full | Molecular characterization and expression analysis of pitaya (Hylocereus polyrhizus) HpLRR genes in response to Neoscytalidium dimidiatum infection |
| title_fullStr | Molecular characterization and expression analysis of pitaya (Hylocereus polyrhizus) HpLRR genes in response to Neoscytalidium dimidiatum infection |
| title_full_unstemmed | Molecular characterization and expression analysis of pitaya (Hylocereus polyrhizus) HpLRR genes in response to Neoscytalidium dimidiatum infection |
| title_short | Molecular characterization and expression analysis of pitaya (Hylocereus polyrhizus) HpLRR genes in response to Neoscytalidium dimidiatum infection |
| title_sort | molecular characterization and expression analysis of pitaya hylocereus polyrhizus hplrr genes in response to neoscytalidium dimidiatum infection |
| topic | Pitaya Canker disease Neoscytalidium dimidiatum Transcriptomics Leucine-rich-repeat genes Expression analysis |
| url | http://link.springer.com/article/10.1186/s12870-020-02368-6 |
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