Bypass of Dfi1 Regulation of Candida albicans Invasive Filamentation by Iron Limitation
ABSTRACT Candida albicans filamentation, the ability to convert from oval yeast cells to elongated hyphal cells, is a key factor in its pathogenesis. Previous work has shown that the integral membrane protein Dfi1 is required for filamentation in cells grown in contact with a semisolid surface. Inve...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2022-02-01
|
| Series: | mSphere |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/msphere.00779-21 |
| _version_ | 1818979848202223616 |
|---|---|
| author | Ashlee Junier Anne Weeks Ysabella Alcaraz Carol A. Kumamoto |
| author_facet | Ashlee Junier Anne Weeks Ysabella Alcaraz Carol A. Kumamoto |
| author_sort | Ashlee Junier |
| collection | DOAJ |
| description | ABSTRACT Candida albicans filamentation, the ability to convert from oval yeast cells to elongated hyphal cells, is a key factor in its pathogenesis. Previous work has shown that the integral membrane protein Dfi1 is required for filamentation in cells grown in contact with a semisolid surface. Investigations into the downstream targets of the Dfi1 pathway revealed potential links to two transcription factors, Sef1 and Czf1. Sef1 regulates iron uptake and iron utilization genes under low-iron conditions, leading us to hypothesize that there exists a link between iron availability and contact-dependent invasive filamentation. In this study, we showed that Sef1 was not required for contact-dependent filamentation, but it was required for wild-type (WT) expression levels of a number of genes during growth under contact conditions. Czf1 is required for contact-dependent filamentation and for WT levels of expression of several genes. Constitutive expression and activation of either Sef1 or Czf1 individually in a dfi1 null strain resulted in a complete rescue of the dfi1 null filamentation defect. Because Sef1 is normally activated in low-iron environments, we embedded WT and dfi1 null cells in iron-free agar medium supplemented with various concentrations of ferrous ammonium sulfate (FAS). dfi1 null cells embedded in media with a low concentration of iron (20 μM FAS) showed increased filamentation in comparison to mutant cells embedded in higher concentrations of iron (50 to 500 μM). WT cells produced filamentous colonies in all concentrations. Together, the data indicate that Dfi1, Czf1, Sef1, and environmental iron regulate C. albicans contact-dependent filamentation. IMPORTANCE Candida albicans is an opportunistic pathogen responsible for a larger proportion of candidiasis and candidemia cases than any other Candida species. The ability of C. albicans cells to invade and cause disease is linked to their ability to filament. Despite this, there are gaps in our knowledge of the environmental cues and intracellular signaling that triggers the switch from commensal organism to filamentous pathogen. In this study, we identified a link between contact-dependent filamentation and iron availability. Over the course of tissue invasion, C. albicans cells encounter a number of different iron microenvironments, from the iron-rich gut to iron-poor tissues. Increased expression of Sef1-dependent iron uptake genes as a result of contact-dependent signaling will promote the adaptation of C. albicans cells to a low-iron-availability environment. |
| first_indexed | 2024-12-20T17:06:03Z |
| format | Article |
| id | doaj.art-ccd08401ee7344618670111f7b793a6f |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-20T17:06:03Z |
| publishDate | 2022-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-ccd08401ee7344618670111f7b793a6f2022-12-21T19:32:17ZengAmerican Society for MicrobiologymSphere2379-50422022-02-017110.1128/msphere.00779-21Bypass of Dfi1 Regulation of Candida albicans Invasive Filamentation by Iron LimitationAshlee Junier0Anne Weeks1Ysabella Alcaraz2Carol A. Kumamoto3Graduate School of Biomedical Sciences and Department of Molecular Biology and Microbiology, Tufts University, Boston, Massachusetts, USAGraduate School of Biomedical Sciences and Department of Molecular Biology and Microbiology, Tufts University, Boston, Massachusetts, USAGraduate School of Biomedical Sciences and Department of Molecular Biology and Microbiology, Tufts University, Boston, Massachusetts, USAGraduate School of Biomedical Sciences and Department of Molecular Biology and Microbiology, Tufts University, Boston, Massachusetts, USAABSTRACT Candida albicans filamentation, the ability to convert from oval yeast cells to elongated hyphal cells, is a key factor in its pathogenesis. Previous work has shown that the integral membrane protein Dfi1 is required for filamentation in cells grown in contact with a semisolid surface. Investigations into the downstream targets of the Dfi1 pathway revealed potential links to two transcription factors, Sef1 and Czf1. Sef1 regulates iron uptake and iron utilization genes under low-iron conditions, leading us to hypothesize that there exists a link between iron availability and contact-dependent invasive filamentation. In this study, we showed that Sef1 was not required for contact-dependent filamentation, but it was required for wild-type (WT) expression levels of a number of genes during growth under contact conditions. Czf1 is required for contact-dependent filamentation and for WT levels of expression of several genes. Constitutive expression and activation of either Sef1 or Czf1 individually in a dfi1 null strain resulted in a complete rescue of the dfi1 null filamentation defect. Because Sef1 is normally activated in low-iron environments, we embedded WT and dfi1 null cells in iron-free agar medium supplemented with various concentrations of ferrous ammonium sulfate (FAS). dfi1 null cells embedded in media with a low concentration of iron (20 μM FAS) showed increased filamentation in comparison to mutant cells embedded in higher concentrations of iron (50 to 500 μM). WT cells produced filamentous colonies in all concentrations. Together, the data indicate that Dfi1, Czf1, Sef1, and environmental iron regulate C. albicans contact-dependent filamentation. IMPORTANCE Candida albicans is an opportunistic pathogen responsible for a larger proportion of candidiasis and candidemia cases than any other Candida species. The ability of C. albicans cells to invade and cause disease is linked to their ability to filament. Despite this, there are gaps in our knowledge of the environmental cues and intracellular signaling that triggers the switch from commensal organism to filamentous pathogen. In this study, we identified a link between contact-dependent filamentation and iron availability. Over the course of tissue invasion, C. albicans cells encounter a number of different iron microenvironments, from the iron-rich gut to iron-poor tissues. Increased expression of Sef1-dependent iron uptake genes as a result of contact-dependent signaling will promote the adaptation of C. albicans cells to a low-iron-availability environment.https://journals.asm.org/doi/10.1128/msphere.00779-21Candida albicansCzf1Sef1filamentationinvasion |
| spellingShingle | Ashlee Junier Anne Weeks Ysabella Alcaraz Carol A. Kumamoto Bypass of Dfi1 Regulation of Candida albicans Invasive Filamentation by Iron Limitation mSphere Candida albicans Czf1 Sef1 filamentation invasion |
| title | Bypass of Dfi1 Regulation of Candida albicans Invasive Filamentation by Iron Limitation |
| title_full | Bypass of Dfi1 Regulation of Candida albicans Invasive Filamentation by Iron Limitation |
| title_fullStr | Bypass of Dfi1 Regulation of Candida albicans Invasive Filamentation by Iron Limitation |
| title_full_unstemmed | Bypass of Dfi1 Regulation of Candida albicans Invasive Filamentation by Iron Limitation |
| title_short | Bypass of Dfi1 Regulation of Candida albicans Invasive Filamentation by Iron Limitation |
| title_sort | bypass of dfi1 regulation of candida albicans invasive filamentation by iron limitation |
| topic | Candida albicans Czf1 Sef1 filamentation invasion |
| url | https://journals.asm.org/doi/10.1128/msphere.00779-21 |
| work_keys_str_mv | AT ashleejunier bypassofdfi1regulationofcandidaalbicansinvasivefilamentationbyironlimitation AT anneweeks bypassofdfi1regulationofcandidaalbicansinvasivefilamentationbyironlimitation AT ysabellaalcaraz bypassofdfi1regulationofcandidaalbicansinvasivefilamentationbyironlimitation AT carolakumamoto bypassofdfi1regulationofcandidaalbicansinvasivefilamentationbyironlimitation |