A spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme
Abstract The recent prediction of diabetes to be a global pandemic invites a detection strategy preferably non-invasive, and bloodless to manage the disease and the associated complications. Here, we have synthesized chitosan polymer functionalized, organic–inorganic bio-compatible nano-hybrids of M...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-44011-y |
| _version_ | 1797559861652750336 |
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| author | Lopamudra Roy Susmita Mondal Neha Bhattacharyya Ria Ghosh Amrita Banerjee Soumendra Singh Arpita Chattopadhyay Saleh A. Ahmed Rabab S. Jassas Munirah M. Al-Rooqi Ziad Moussa Ismail I. Althagafi Debasish Bhattacharya Kallol Bhattacharya Asim Kumar Mallick Samir Kumar Pal |
| author_facet | Lopamudra Roy Susmita Mondal Neha Bhattacharyya Ria Ghosh Amrita Banerjee Soumendra Singh Arpita Chattopadhyay Saleh A. Ahmed Rabab S. Jassas Munirah M. Al-Rooqi Ziad Moussa Ismail I. Althagafi Debasish Bhattacharya Kallol Bhattacharya Asim Kumar Mallick Samir Kumar Pal |
| author_sort | Lopamudra Roy |
| collection | DOAJ |
| description | Abstract The recent prediction of diabetes to be a global pandemic invites a detection strategy preferably non-invasive, and bloodless to manage the disease and the associated complications. Here, we have synthesized chitosan polymer functionalized, organic–inorganic bio-compatible nano-hybrids of Mn3O4 nanoparticles, and characterized it by utilizing several optical methodologies for the structural characterization which shows the Michaelis Menten (MM) kinetics for glucose and alpha-amylase protein (well-known diabetes biomarkers). We have also studied the potentiality for the detection of alpha-amylase in human salivary secretion which is reported to be strongly correlated with uncontrolled hyperglycemia. Finally, we have developed a prototype for the measurement of glucose (LOD of 0.38 mg/dL, LOQ of 1.15 mg/dL) and HbA1c (LOD of 0.15% and LOQ of 0.45%) utilizing the basic knowledge in the study for the detection of uncontrolled hyperglycemia at the point-of-care. With the limited number of clinical trials, we have explored the potential of our work in combating the diabetic pandemic across the globe in near future. |
| first_indexed | 2024-03-10T17:51:15Z |
| format | Article |
| id | doaj.art-ccd0bb4125ca45c29b734abb8a00af6c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:51:15Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ccd0bb4125ca45c29b734abb8a00af6c2023-11-20T09:21:23ZengNature PortfolioScientific Reports2045-23222023-10-0113111510.1038/s41598-023-44011-yA spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozymeLopamudra Roy0Susmita Mondal1Neha Bhattacharyya2Ria Ghosh3Amrita Banerjee4Soumendra Singh5Arpita Chattopadhyay6Saleh A. Ahmed7Rabab S. Jassas8Munirah M. Al-Rooqi9Ziad Moussa10Ismail I. Althagafi11Debasish Bhattacharya12Kallol Bhattacharya13Asim Kumar Mallick14Samir Kumar Pal15Department of Applied Optics and Photonics, University of CalcuttaDepartment of Chemical and Biological Sciences, S. N. Bose National Centre for Basic SciencesDepartment of Radio Physics and Electronics, University of CalcuttaDepartment of Chemical and Biological Sciences, S. N. Bose National Centre for Basic SciencesDepartment of Physics, Jadavpur UniversityDepartment of Chemical and Biological Sciences, S. N. Bose National Centre for Basic SciencesDepartment of Basic Science and Humanities, Techno International New Town BlockChemistry Department, Faculty of Applied Science, Umm Al-Qura UniversityDepartment of Chemistry, Jamoum University College, Umm Al-Qura UniversityChemistry Department, Faculty of Applied Science, Umm Al-Qura UniversityDepartment of Chemistry, College of Science, United Arab Emirates UniversityChemistry Department, Faculty of Applied Science, Umm Al-Qura UniversityDepartment of Gynecology & Obstetrics, Nil Ratan Sircar Medical College & HospitalDepartment of Applied Optics and Photonics, University of CalcuttaDepartment of Pediatrics, Nil Ratan Sircar Medical College and HospitalDepartment of Chemical and Biological Sciences, S. N. Bose National Centre for Basic SciencesAbstract The recent prediction of diabetes to be a global pandemic invites a detection strategy preferably non-invasive, and bloodless to manage the disease and the associated complications. Here, we have synthesized chitosan polymer functionalized, organic–inorganic bio-compatible nano-hybrids of Mn3O4 nanoparticles, and characterized it by utilizing several optical methodologies for the structural characterization which shows the Michaelis Menten (MM) kinetics for glucose and alpha-amylase protein (well-known diabetes biomarkers). We have also studied the potentiality for the detection of alpha-amylase in human salivary secretion which is reported to be strongly correlated with uncontrolled hyperglycemia. Finally, we have developed a prototype for the measurement of glucose (LOD of 0.38 mg/dL, LOQ of 1.15 mg/dL) and HbA1c (LOD of 0.15% and LOQ of 0.45%) utilizing the basic knowledge in the study for the detection of uncontrolled hyperglycemia at the point-of-care. With the limited number of clinical trials, we have explored the potential of our work in combating the diabetic pandemic across the globe in near future.https://doi.org/10.1038/s41598-023-44011-y |
| spellingShingle | Lopamudra Roy Susmita Mondal Neha Bhattacharyya Ria Ghosh Amrita Banerjee Soumendra Singh Arpita Chattopadhyay Saleh A. Ahmed Rabab S. Jassas Munirah M. Al-Rooqi Ziad Moussa Ismail I. Althagafi Debasish Bhattacharya Kallol Bhattacharya Asim Kumar Mallick Samir Kumar Pal A spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme Scientific Reports |
| title | A spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme |
| title_full | A spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme |
| title_fullStr | A spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme |
| title_full_unstemmed | A spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme |
| title_short | A spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme |
| title_sort | spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme |
| url | https://doi.org/10.1038/s41598-023-44011-y |
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