Bdf1 Bromodomains Are Essential for Meiosis and the Expression of Meiotic-Specific Genes.
Bromodomain and Extra-terminal motif (BET) proteins play a central role in transcription regulation and chromatin signalling pathways. They are present in unicellular eukaryotes and in this study, the role of the BET protein Bdf1 has been explored in Saccharomyces cerevisiae. Mutation of Bdf1 bromod...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC5261807?pdf=render |
| _version_ | 1818203536455892992 |
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| author | Encar García-Oliver Claire Ramus Jonathan Perot Marie Arlotto Morgane Champleboux Flore Mietton Christophe Battail Anne Boland Jean-François Deleuze Myriam Ferro Yohann Couté Jérôme Govin |
| author_facet | Encar García-Oliver Claire Ramus Jonathan Perot Marie Arlotto Morgane Champleboux Flore Mietton Christophe Battail Anne Boland Jean-François Deleuze Myriam Ferro Yohann Couté Jérôme Govin |
| author_sort | Encar García-Oliver |
| collection | DOAJ |
| description | Bromodomain and Extra-terminal motif (BET) proteins play a central role in transcription regulation and chromatin signalling pathways. They are present in unicellular eukaryotes and in this study, the role of the BET protein Bdf1 has been explored in Saccharomyces cerevisiae. Mutation of Bdf1 bromodomains revealed defects on both the formation of spores and the meiotic progression, blocking cells at the exit from prophase, before the first meiotic division. This phenotype is associated with a massive deregulation of the transcription of meiotic genes and Bdf1 bromodomains are required for appropriate expression of the key meiotic transcription factor NDT80 and almost all the Ndt80-inducible genes, including APC complex components. Bdf1 notably accumulates on the promoter of Ndt80 and its recruitment is dependent on Bdf1 bromodomains. In addition, the ectopic expression of NDT80 during meiosis partially bypasses this dependency. Finally, purification of Bdf1 partners identified two independent complexes with Bdf2 or the SWR complex, neither of which was required to complete sporulation. Taken together, our results unveil a new role for Bdf1 -working independently from its predominant protein partners Bdf2 and the SWR1 complex-as a regulator of meiosis-specific genes. |
| first_indexed | 2024-12-12T03:26:54Z |
| format | Article |
| id | doaj.art-ccd126a24df94810b555e05b63fbaa6e |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-12T03:26:54Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-ccd126a24df94810b555e05b63fbaa6e2022-12-22T00:40:01ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042017-01-01131e100654110.1371/journal.pgen.1006541Bdf1 Bromodomains Are Essential for Meiosis and the Expression of Meiotic-Specific Genes.Encar García-OliverClaire RamusJonathan PerotMarie ArlottoMorgane ChamplebouxFlore MiettonChristophe BattailAnne BolandJean-François DeleuzeMyriam FerroYohann CoutéJérôme GovinBromodomain and Extra-terminal motif (BET) proteins play a central role in transcription regulation and chromatin signalling pathways. They are present in unicellular eukaryotes and in this study, the role of the BET protein Bdf1 has been explored in Saccharomyces cerevisiae. Mutation of Bdf1 bromodomains revealed defects on both the formation of spores and the meiotic progression, blocking cells at the exit from prophase, before the first meiotic division. This phenotype is associated with a massive deregulation of the transcription of meiotic genes and Bdf1 bromodomains are required for appropriate expression of the key meiotic transcription factor NDT80 and almost all the Ndt80-inducible genes, including APC complex components. Bdf1 notably accumulates on the promoter of Ndt80 and its recruitment is dependent on Bdf1 bromodomains. In addition, the ectopic expression of NDT80 during meiosis partially bypasses this dependency. Finally, purification of Bdf1 partners identified two independent complexes with Bdf2 or the SWR complex, neither of which was required to complete sporulation. Taken together, our results unveil a new role for Bdf1 -working independently from its predominant protein partners Bdf2 and the SWR1 complex-as a regulator of meiosis-specific genes.http://europepmc.org/articles/PMC5261807?pdf=render |
| spellingShingle | Encar García-Oliver Claire Ramus Jonathan Perot Marie Arlotto Morgane Champleboux Flore Mietton Christophe Battail Anne Boland Jean-François Deleuze Myriam Ferro Yohann Couté Jérôme Govin Bdf1 Bromodomains Are Essential for Meiosis and the Expression of Meiotic-Specific Genes. PLoS Genetics |
| title | Bdf1 Bromodomains Are Essential for Meiosis and the Expression of Meiotic-Specific Genes. |
| title_full | Bdf1 Bromodomains Are Essential for Meiosis and the Expression of Meiotic-Specific Genes. |
| title_fullStr | Bdf1 Bromodomains Are Essential for Meiosis and the Expression of Meiotic-Specific Genes. |
| title_full_unstemmed | Bdf1 Bromodomains Are Essential for Meiosis and the Expression of Meiotic-Specific Genes. |
| title_short | Bdf1 Bromodomains Are Essential for Meiosis and the Expression of Meiotic-Specific Genes. |
| title_sort | bdf1 bromodomains are essential for meiosis and the expression of meiotic specific genes |
| url | http://europepmc.org/articles/PMC5261807?pdf=render |
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