Molecular Analysis of Anti-Tuberculosis Drug Resistance of <i>Mycobacterium tuberculosis</i> Isolated in the Republic of Korea
Rapid and accurate detection of tuberculosis (TB) drug resistance is critical for the successful treatment and control of TB. Here, we investigated resistance to anti-TB drugs and genetic variations in 215 drug-resistant <i>Mycobacterium tuberculosis</i> isolates in Korea. Genetic variat...
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MDPI AG
2023-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/8/1324 |
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| author | Se-Mi Jeon Sanghee Park Na-Ra Lim Noori Lee Jihee Jung Nackmoon Sung Seonghan Kim |
| author_facet | Se-Mi Jeon Sanghee Park Na-Ra Lim Noori Lee Jihee Jung Nackmoon Sung Seonghan Kim |
| author_sort | Se-Mi Jeon |
| collection | DOAJ |
| description | Rapid and accurate detection of tuberculosis (TB) drug resistance is critical for the successful treatment and control of TB. Here, we investigated resistance to anti-TB drugs and genetic variations in 215 drug-resistant <i>Mycobacterium tuberculosis</i> isolates in Korea. Genetic variations were observed in <i>rpoB</i> Ser531Leu, <i>katG</i> Ser315Thr, and <i>gyrA</i> Asp94Gly; however, the minimum inhibitory concentrations varied, which can be attributed to other resistance mechanisms. Examination of genetic relatedness among drug-resistant isolates revealed that the cluster size of resistant bacteria was less than six strains, suggesting no evidence of a large-scale epidemic caused by a specific strain. However, <i>rpoC</i> mutants of the rifampicin-resistant isolates were composed of five types of clusters, suggesting that these compensatory mutations advance propagation. In the present study, more than 90% of the resistance mechanisms to major anti-TB drugs were identified, and the effect of each mutation on drug resistance was estimated. With the clinical application of recent next-generation sequencing-based susceptibility testing, the present study is expected to improve the clinical utilization of genotype-based drug susceptibility testing for the diagnosis and treatment of patients with drug-resistant TB. |
| first_indexed | 2024-03-11T00:10:55Z |
| format | Article |
| id | doaj.art-ccd37291e0e44048816f7731b02c15a2 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T00:10:55Z |
| publishDate | 2023-08-01 |
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| series | Antibiotics |
| spelling | doaj.art-ccd37291e0e44048816f7731b02c15a22023-11-19T00:00:00ZengMDPI AGAntibiotics2079-63822023-08-01128132410.3390/antibiotics12081324Molecular Analysis of Anti-Tuberculosis Drug Resistance of <i>Mycobacterium tuberculosis</i> Isolated in the Republic of KoreaSe-Mi Jeon0Sanghee Park1Na-Ra Lim2Noori Lee3Jihee Jung4Nackmoon Sung5Seonghan Kim6Division of Bacterial Disease Research, Korea National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju-si 28159, Republic of KoreaDivision of Bacterial Disease Research, Korea National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju-si 28159, Republic of KoreaDivision of Bacterial Disease Research, Korea National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju-si 28159, Republic of KoreaClinical Research Center, Masan National Tuberculosis Hospital, Changwon-si 51755, Republic of KoreaClinical Research Center, Masan National Tuberculosis Hospital, Changwon-si 51755, Republic of KoreaClinical Research Center, Masan National Tuberculosis Hospital, Changwon-si 51755, Republic of KoreaDivision of Bacterial Disease Research, Korea National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju-si 28159, Republic of KoreaRapid and accurate detection of tuberculosis (TB) drug resistance is critical for the successful treatment and control of TB. Here, we investigated resistance to anti-TB drugs and genetic variations in 215 drug-resistant <i>Mycobacterium tuberculosis</i> isolates in Korea. Genetic variations were observed in <i>rpoB</i> Ser531Leu, <i>katG</i> Ser315Thr, and <i>gyrA</i> Asp94Gly; however, the minimum inhibitory concentrations varied, which can be attributed to other resistance mechanisms. Examination of genetic relatedness among drug-resistant isolates revealed that the cluster size of resistant bacteria was less than six strains, suggesting no evidence of a large-scale epidemic caused by a specific strain. However, <i>rpoC</i> mutants of the rifampicin-resistant isolates were composed of five types of clusters, suggesting that these compensatory mutations advance propagation. In the present study, more than 90% of the resistance mechanisms to major anti-TB drugs were identified, and the effect of each mutation on drug resistance was estimated. With the clinical application of recent next-generation sequencing-based susceptibility testing, the present study is expected to improve the clinical utilization of genotype-based drug susceptibility testing for the diagnosis and treatment of patients with drug-resistant TB.https://www.mdpi.com/2079-6382/12/8/1324<i>Mycobacterium tuberculosis</i>drug resistanceDST24-locus MIRU-VNTRspoligotyping |
| spellingShingle | Se-Mi Jeon Sanghee Park Na-Ra Lim Noori Lee Jihee Jung Nackmoon Sung Seonghan Kim Molecular Analysis of Anti-Tuberculosis Drug Resistance of <i>Mycobacterium tuberculosis</i> Isolated in the Republic of Korea Antibiotics <i>Mycobacterium tuberculosis</i> drug resistance DST 24-locus MIRU-VNTR spoligotyping |
| title | Molecular Analysis of Anti-Tuberculosis Drug Resistance of <i>Mycobacterium tuberculosis</i> Isolated in the Republic of Korea |
| title_full | Molecular Analysis of Anti-Tuberculosis Drug Resistance of <i>Mycobacterium tuberculosis</i> Isolated in the Republic of Korea |
| title_fullStr | Molecular Analysis of Anti-Tuberculosis Drug Resistance of <i>Mycobacterium tuberculosis</i> Isolated in the Republic of Korea |
| title_full_unstemmed | Molecular Analysis of Anti-Tuberculosis Drug Resistance of <i>Mycobacterium tuberculosis</i> Isolated in the Republic of Korea |
| title_short | Molecular Analysis of Anti-Tuberculosis Drug Resistance of <i>Mycobacterium tuberculosis</i> Isolated in the Republic of Korea |
| title_sort | molecular analysis of anti tuberculosis drug resistance of i mycobacterium tuberculosis i isolated in the republic of korea |
| topic | <i>Mycobacterium tuberculosis</i> drug resistance DST 24-locus MIRU-VNTR spoligotyping |
| url | https://www.mdpi.com/2079-6382/12/8/1324 |
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