Biosensors Used for Epifluorescence and Confocal Laser Scanning Microscopies to Study <i>Dickeya</i> and <i>Pectobacterium</i> Virulence and Biocontrol
Promoter-probe vectors carrying fluorescent protein-reporter genes are powerful tools used to study microbial ecology, epidemiology, and etiology. In addition, they provide direct visual evidence of molecular interactions related to cell physiology and metabolism. Knowledge and advances carried out...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/295 |
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| author | Yvann Bourigault Andrea Chane Corinne Barbey Sylwia Jafra Robert Czajkowski Xavier Latour |
| author_facet | Yvann Bourigault Andrea Chane Corinne Barbey Sylwia Jafra Robert Czajkowski Xavier Latour |
| author_sort | Yvann Bourigault |
| collection | DOAJ |
| description | Promoter-probe vectors carrying fluorescent protein-reporter genes are powerful tools used to study microbial ecology, epidemiology, and etiology. In addition, they provide direct visual evidence of molecular interactions related to cell physiology and metabolism. Knowledge and advances carried out thanks to the construction of soft-rot <i>Pectobacteriaceae</i> biosensors, often inoculated in potato <i>Solanum tuberosum</i>, are discussed in this review. Under epifluorescence and confocal laser scanning microscopies, <i>Dickeya</i> and <i>Pectobacterium</i>-tagged strains managed to monitor in situ bacterial viability, microcolony and biofilm formation, and colonization of infected plant organs, as well as disease symptoms, such as cell-wall lysis and their suppression by biocontrol antagonists. The use of dual-colored reporters encoding the first fluorophore expressed from a constitutive promoter as a cell tag, while a second was used as a regulator-based reporter system, was also used to simultaneously visualize bacterial spread and activity. This revealed the chronology of events leading to tuber maceration and quorum-sensing communication, in addition to the disruption of the latter by biocontrol agents. The promising potential of these fluorescent biosensors should make it possible to apprehend other activities, such as subcellular localization of key proteins involved in bacterial virulence <i>in planta</i>, in the near future. |
| first_indexed | 2024-03-09T06:09:45Z |
| format | Article |
| id | doaj.art-ccd42f33037c41e28823e66dfc090e95 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T06:09:45Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ccd42f33037c41e28823e66dfc090e952023-12-03T11:59:45ZengMDPI AGMicroorganisms2076-26072021-02-019229510.3390/microorganisms9020295Biosensors Used for Epifluorescence and Confocal Laser Scanning Microscopies to Study <i>Dickeya</i> and <i>Pectobacterium</i> Virulence and BiocontrolYvann Bourigault0Andrea Chane1Corinne Barbey2Sylwia Jafra3Robert Czajkowski4Xavier Latour5Laboratory of Microbiology Signals and Microenvironment (LMSM EA 4312), University of Rouen Normandy, 55 rue Saint-Germain, F-27000 Evreux, FranceLaboratory of Microbiology Signals and Microenvironment (LMSM EA 4312), University of Rouen Normandy, 55 rue Saint-Germain, F-27000 Evreux, FranceLaboratory of Microbiology Signals and Microenvironment (LMSM EA 4312), University of Rouen Normandy, 55 rue Saint-Germain, F-27000 Evreux, FranceDivision of Biological Plant Protection, Intercollegiate Faculty of Biotechnology UG and MUG, University of Gdansk, ul. A. Abrahama 58, 80-307 Gdansk, PolandDivision of Biologically Active Compounds, Intercollegiate Faculty of Biotechnology UG and MUG, University of Gdansk, ul. A. Abrahama 58, 80-307 Gdansk, PolandLaboratory of Microbiology Signals and Microenvironment (LMSM EA 4312), University of Rouen Normandy, 55 rue Saint-Germain, F-27000 Evreux, FrancePromoter-probe vectors carrying fluorescent protein-reporter genes are powerful tools used to study microbial ecology, epidemiology, and etiology. In addition, they provide direct visual evidence of molecular interactions related to cell physiology and metabolism. Knowledge and advances carried out thanks to the construction of soft-rot <i>Pectobacteriaceae</i> biosensors, often inoculated in potato <i>Solanum tuberosum</i>, are discussed in this review. Under epifluorescence and confocal laser scanning microscopies, <i>Dickeya</i> and <i>Pectobacterium</i>-tagged strains managed to monitor in situ bacterial viability, microcolony and biofilm formation, and colonization of infected plant organs, as well as disease symptoms, such as cell-wall lysis and their suppression by biocontrol antagonists. The use of dual-colored reporters encoding the first fluorophore expressed from a constitutive promoter as a cell tag, while a second was used as a regulator-based reporter system, was also used to simultaneously visualize bacterial spread and activity. This revealed the chronology of events leading to tuber maceration and quorum-sensing communication, in addition to the disruption of the latter by biocontrol agents. The promising potential of these fluorescent biosensors should make it possible to apprehend other activities, such as subcellular localization of key proteins involved in bacterial virulence <i>in planta</i>, in the near future.https://www.mdpi.com/2076-2607/9/2/295fluorescent proteinsgreen fluorescent protein (GFP)microscopyplant colonization<i>Dickeya</i><i>Pectobacterium</i> |
| spellingShingle | Yvann Bourigault Andrea Chane Corinne Barbey Sylwia Jafra Robert Czajkowski Xavier Latour Biosensors Used for Epifluorescence and Confocal Laser Scanning Microscopies to Study <i>Dickeya</i> and <i>Pectobacterium</i> Virulence and Biocontrol Microorganisms fluorescent proteins green fluorescent protein (GFP) microscopy plant colonization <i>Dickeya</i> <i>Pectobacterium</i> |
| title | Biosensors Used for Epifluorescence and Confocal Laser Scanning Microscopies to Study <i>Dickeya</i> and <i>Pectobacterium</i> Virulence and Biocontrol |
| title_full | Biosensors Used for Epifluorescence and Confocal Laser Scanning Microscopies to Study <i>Dickeya</i> and <i>Pectobacterium</i> Virulence and Biocontrol |
| title_fullStr | Biosensors Used for Epifluorescence and Confocal Laser Scanning Microscopies to Study <i>Dickeya</i> and <i>Pectobacterium</i> Virulence and Biocontrol |
| title_full_unstemmed | Biosensors Used for Epifluorescence and Confocal Laser Scanning Microscopies to Study <i>Dickeya</i> and <i>Pectobacterium</i> Virulence and Biocontrol |
| title_short | Biosensors Used for Epifluorescence and Confocal Laser Scanning Microscopies to Study <i>Dickeya</i> and <i>Pectobacterium</i> Virulence and Biocontrol |
| title_sort | biosensors used for epifluorescence and confocal laser scanning microscopies to study i dickeya i and i pectobacterium i virulence and biocontrol |
| topic | fluorescent proteins green fluorescent protein (GFP) microscopy plant colonization <i>Dickeya</i> <i>Pectobacterium</i> |
| url | https://www.mdpi.com/2076-2607/9/2/295 |
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