Comparison of inducible nitric oxide synthase activity in pancreatic islets of young and aged rats
Objective(s):Some pathologic situations such as diabetes and metabolic syndrome are associated with alternation in nitric oxide level. Incidence of these condition increases with aging. On the other hand, insulin secretion is modulated by nitric oxide, and nitric oxide synthase (NOS) activity is als...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2015-02-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_4011_e5ee4e7fe857b9a9cb3844bc5490cf55.html |
| _version_ | 1818808150148513792 |
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| author | Khadije Farrokhfall Mehri Seyed Hashtroudi Asghar Ghasemi Hossein Mehrani |
| author_facet | Khadije Farrokhfall Mehri Seyed Hashtroudi Asghar Ghasemi Hossein Mehrani |
| author_sort | Khadije Farrokhfall |
| collection | DOAJ |
| description | Objective(s):Some pathologic situations such as diabetes and metabolic syndrome are associated with alternation in nitric oxide level. Incidence of these condition increases with aging. On the other hand, insulin secretion is modulated by nitric oxide, and nitric oxide synthase (NOS) activity is also altered in diabetes. In this study, modification in the enzyme activity associated with aging and also optimized procedure for islet NOS assay was investigated.
Materials and Methods: Male Wistar rats were randomly divided in two experimental groups: A: adult rats; were 4 month old and B: old rats; were 12 month old. In all groups, plasma glucose, insulin and NOX (nitrite + nitrate = NOX) were measured, and also insulin secretion in isolated pancreatic islet with or without L-NAME was investigated. Furthermore, the inducible NOS activity with L-citrulline measurement in islets was measured.
Results: L-citrulline was quantified using one step HPLC column. Aging induced hyperglycemia (P |
| first_indexed | 2024-12-18T19:36:59Z |
| format | Article |
| id | doaj.art-ccd5c2c925904468a34982dba0665913 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-18T19:36:59Z |
| publishDate | 2015-02-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-ccd5c2c925904468a34982dba06659132022-12-21T20:55:35ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742015-02-011821151214011Comparison of inducible nitric oxide synthase activity in pancreatic islets of young and aged ratsKhadije Farrokhfall0Mehri Seyed Hashtroudi1Asghar Ghasemi2Hossein Mehrani3Department of Physiology and Biophysics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, IranDepartment of Marine Living Sciences, Iranian National Institute for Oceanography and Atmospheric Sciences (INIOAS), Tehran, IranEndocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IranChemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranObjective(s):Some pathologic situations such as diabetes and metabolic syndrome are associated with alternation in nitric oxide level. Incidence of these condition increases with aging. On the other hand, insulin secretion is modulated by nitric oxide, and nitric oxide synthase (NOS) activity is also altered in diabetes. In this study, modification in the enzyme activity associated with aging and also optimized procedure for islet NOS assay was investigated. Materials and Methods: Male Wistar rats were randomly divided in two experimental groups: A: adult rats; were 4 month old and B: old rats; were 12 month old. In all groups, plasma glucose, insulin and NOX (nitrite + nitrate = NOX) were measured, and also insulin secretion in isolated pancreatic islet with or without L-NAME was investigated. Furthermore, the inducible NOS activity with L-citrulline measurement in islets was measured. Results: L-citrulline was quantified using one step HPLC column. Aging induced hyperglycemia (Phttp://ijbms.mums.ac.ir/pdf_4011_e5ee4e7fe857b9a9cb3844bc5490cf55.htmlAging High performance liquid chromato-graphy Islet L-Citrulline Nitric oxide synthase activity Rat |
| spellingShingle | Khadije Farrokhfall Mehri Seyed Hashtroudi Asghar Ghasemi Hossein Mehrani Comparison of inducible nitric oxide synthase activity in pancreatic islets of young and aged rats Iranian Journal of Basic Medical Sciences Aging High performance liquid chromato-graphy Islet L-Citrulline Nitric oxide synthase activity Rat |
| title | Comparison of inducible nitric oxide synthase activity in pancreatic islets of young and aged rats |
| title_full | Comparison of inducible nitric oxide synthase activity in pancreatic islets of young and aged rats |
| title_fullStr | Comparison of inducible nitric oxide synthase activity in pancreatic islets of young and aged rats |
| title_full_unstemmed | Comparison of inducible nitric oxide synthase activity in pancreatic islets of young and aged rats |
| title_short | Comparison of inducible nitric oxide synthase activity in pancreatic islets of young and aged rats |
| title_sort | comparison of inducible nitric oxide synthase activity in pancreatic islets of young and aged rats |
| topic | Aging High performance liquid chromato-graphy Islet L-Citrulline Nitric oxide synthase activity Rat |
| url | http://ijbms.mums.ac.ir/pdf_4011_e5ee4e7fe857b9a9cb3844bc5490cf55.html |
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