Mutant-Dependent Local Orientational Correlation in Biofilms of <i>Vibrio campbellii</i> Revealed through Digital Processing of Light Microscopy Images
Biofilms are key bacterial communities in genetic and adaptive resistance to antibiotics as well as disease control strategies. The mature high-coverage biofilm formations of the <i>Vibrio campbellii</i> strains (wild type BB120 and isogenic derivatives JAF633, KM387, and JMH603) are stu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5423 |
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| author | Maura Cesaria Matteo Calcagnile Pietro Alifano Rosella Cataldo |
| author_facet | Maura Cesaria Matteo Calcagnile Pietro Alifano Rosella Cataldo |
| author_sort | Maura Cesaria |
| collection | DOAJ |
| description | Biofilms are key bacterial communities in genetic and adaptive resistance to antibiotics as well as disease control strategies. The mature high-coverage biofilm formations of the <i>Vibrio campbellii</i> strains (wild type BB120 and isogenic derivatives JAF633, KM387, and JMH603) are studied here through the unstraightforward digital processing of morphologically complex images without segmentation or the unrealistic simplifications used to artificially simulate low-density formations. The main results concern the specific mutant- and coverage-dependent short-range orientational correlation as well as the coherent development of biofilm growth pathways over the subdomains of the image. These findings are demonstrated to be unthinkable based only on a visual inspection of the samples or on methods such as Voronoi tessellation or correlation analyses. The presented approach is general, relies on measured rather than simulated low-density formations, and could be employed in the development of a highly efficient screening method for drugs or innovative materials. |
| first_indexed | 2024-03-11T06:26:27Z |
| format | Article |
| id | doaj.art-ccd96c39300841499e79cf0a6d4cc6f7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:26:27Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ccd96c39300841499e79cf0a6d4cc6f72023-11-17T11:33:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246542310.3390/ijms24065423Mutant-Dependent Local Orientational Correlation in Biofilms of <i>Vibrio campbellii</i> Revealed through Digital Processing of Light Microscopy ImagesMaura Cesaria0Matteo Calcagnile1Pietro Alifano2Rosella Cataldo3Department of Mathematics and Physics Ennio De Giorgi, University of Salento-c/o Campus Ecotekne, Via per Arnesano, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies (Di.S.Te.BA.), University of Salento-c/o Campus Ecotekne—S.P. 6, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies (Di.S.Te.BA.), University of Salento-c/o Campus Ecotekne—S.P. 6, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies (Di.S.Te.BA.), University of Salento-c/o Campus Ecotekne—S.P. 6, 73100 Lecce, ItalyBiofilms are key bacterial communities in genetic and adaptive resistance to antibiotics as well as disease control strategies. The mature high-coverage biofilm formations of the <i>Vibrio campbellii</i> strains (wild type BB120 and isogenic derivatives JAF633, KM387, and JMH603) are studied here through the unstraightforward digital processing of morphologically complex images without segmentation or the unrealistic simplifications used to artificially simulate low-density formations. The main results concern the specific mutant- and coverage-dependent short-range orientational correlation as well as the coherent development of biofilm growth pathways over the subdomains of the image. These findings are demonstrated to be unthinkable based only on a visual inspection of the samples or on methods such as Voronoi tessellation or correlation analyses. The presented approach is general, relies on measured rather than simulated low-density formations, and could be employed in the development of a highly efficient screening method for drugs or innovative materials.https://www.mdpi.com/1422-0067/24/6/5423<i>Vibrio campbellii</i> strainshigh-coverage mature biofilmslight microscopy imagingdigital image processingcorrelation statisticsmutant-dependent oriented subdomains |
| spellingShingle | Maura Cesaria Matteo Calcagnile Pietro Alifano Rosella Cataldo Mutant-Dependent Local Orientational Correlation in Biofilms of <i>Vibrio campbellii</i> Revealed through Digital Processing of Light Microscopy Images International Journal of Molecular Sciences <i>Vibrio campbellii</i> strains high-coverage mature biofilms light microscopy imaging digital image processing correlation statistics mutant-dependent oriented subdomains |
| title | Mutant-Dependent Local Orientational Correlation in Biofilms of <i>Vibrio campbellii</i> Revealed through Digital Processing of Light Microscopy Images |
| title_full | Mutant-Dependent Local Orientational Correlation in Biofilms of <i>Vibrio campbellii</i> Revealed through Digital Processing of Light Microscopy Images |
| title_fullStr | Mutant-Dependent Local Orientational Correlation in Biofilms of <i>Vibrio campbellii</i> Revealed through Digital Processing of Light Microscopy Images |
| title_full_unstemmed | Mutant-Dependent Local Orientational Correlation in Biofilms of <i>Vibrio campbellii</i> Revealed through Digital Processing of Light Microscopy Images |
| title_short | Mutant-Dependent Local Orientational Correlation in Biofilms of <i>Vibrio campbellii</i> Revealed through Digital Processing of Light Microscopy Images |
| title_sort | mutant dependent local orientational correlation in biofilms of i vibrio campbellii i revealed through digital processing of light microscopy images |
| topic | <i>Vibrio campbellii</i> strains high-coverage mature biofilms light microscopy imaging digital image processing correlation statistics mutant-dependent oriented subdomains |
| url | https://www.mdpi.com/1422-0067/24/6/5423 |
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