<i>Piriformospora indica</i> Primes Onion Response against Stemphylium Leaf Blight Disease
The root-endophytic fungus <i>Piriformospora indica</i> (=<i>Serendipita indica</i>) has been revealed for its growth-promoting effects and its capacity to induce resistance in a broad spectrum of host plants. However, the bioefficacy of this fungus had not yet been tested ag...
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MDPI AG
2021-08-01
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| author | Praveen Roylawar Kiran Khandagale Pragati Randive Bharat Shinde Chandrashekhar Murumkar Avinash Ade Major Singh Suresh Gawande Massimiliano Morelli |
| author_facet | Praveen Roylawar Kiran Khandagale Pragati Randive Bharat Shinde Chandrashekhar Murumkar Avinash Ade Major Singh Suresh Gawande Massimiliano Morelli |
| author_sort | Praveen Roylawar |
| collection | DOAJ |
| description | The root-endophytic fungus <i>Piriformospora indica</i> (=<i>Serendipita indica</i>) has been revealed for its growth-promoting effects and its capacity to induce resistance in a broad spectrum of host plants. However, the bioefficacy of this fungus had not yet been tested against any pathogen affecting onion (<i>Allium cepa</i>). In this study, the biocontrol potency of <i>P. indica</i> against onion leaf blight, an impacting disease caused by the necrotrophic fungal pathogen <i>Stemphylium vesicarium</i>, was evaluated. First, it was proved that colonisation of onion roots by <i>P. indica</i> was beneficial for plant growth, as it increased leaf development and root biomass. Most relevantly, <i>P. indica</i> was also effective in reducing Stemphylium leaf blight (SLB) severity, as assessed under greenhouse conditions and confirmed in field trials in two consecutive years. These investigations could also provide some insight into the biochemical and molecular changes that treatment with <i>P. indica</i> induces in the main pathways associated with host defence response. It was possible to highlight the protective effect of <i>P. indica</i> colonisation against peroxidative damage, and its role in signalling oxidative stress, by assessing changes in malondialdehyde and H<sub>2</sub>O<sub>2</sub> content. It was also showed that treatment with <i>P. indica</i> contributes to modulate the enzymatic activity of superoxide dismutase, catalase, phenylalanine ammonia-lyase and peroxidase, in the course of infection. qPCR-based expression analysis of defence-related genes <i>AcLOX1</i>, <i>AcLOX2</i>, <i>AcPAL1</i>, <i>AcGST</i>, <i>AcCHI</i>, <i>AcWRKY1,</i> and <i>AcWRKY70</i> provided further indications on <i>P. indica</i> ability to induce onion systemic response. Based on the evidence gathered, this study aims to propose <i>P. indica</i> application as a sustainable tool for improving SLB control, which might not only enhance onion growth performance but also activate defence signalling mechanisms more effectively, involving different pathways. |
| first_indexed | 2024-03-10T07:20:39Z |
| format | Article |
| id | doaj.art-ccdae461a8a5428785d6cb7316a40c3a |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T07:20:39Z |
| publishDate | 2021-08-01 |
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| series | Pathogens |
| spelling | doaj.art-ccdae461a8a5428785d6cb7316a40c3a2023-11-22T14:41:29ZengMDPI AGPathogens2076-08172021-08-01109108510.3390/pathogens10091085<i>Piriformospora indica</i> Primes Onion Response against Stemphylium Leaf Blight DiseasePraveen Roylawar0Kiran Khandagale1Pragati Randive2Bharat Shinde3Chandrashekhar Murumkar4Avinash Ade5Major Singh6Suresh Gawande7Massimiliano Morelli8ICAR-Directorate of Onion and Garlic Research (DOGR), Rajgurunagar, Pune 410505, IndiaDepartment of Botany, Savitribai Phule Pune University, Pune 411007, IndiaICAR-Directorate of Onion and Garlic Research (DOGR), Rajgurunagar, Pune 410505, IndiaVidya Pratishthan’s Arts, Science & Commerce College, Baramati, Pune 413133, IndiaTuljaram Chaturchand College of Arts, Science and Commerce, Baramati, Pune 413102, IndiaDepartment of Botany, Savitribai Phule Pune University, Pune 411007, IndiaICAR-Directorate of Onion and Garlic Research (DOGR), Rajgurunagar, Pune 410505, IndiaICAR-Directorate of Onion and Garlic Research (DOGR), Rajgurunagar, Pune 410505, IndiaCNR-IPSP Istituto per la Protezione Sostenibile delle Piante, Sede Secondaria di Bari, 70124 Bari, ItalyThe root-endophytic fungus <i>Piriformospora indica</i> (=<i>Serendipita indica</i>) has been revealed for its growth-promoting effects and its capacity to induce resistance in a broad spectrum of host plants. However, the bioefficacy of this fungus had not yet been tested against any pathogen affecting onion (<i>Allium cepa</i>). In this study, the biocontrol potency of <i>P. indica</i> against onion leaf blight, an impacting disease caused by the necrotrophic fungal pathogen <i>Stemphylium vesicarium</i>, was evaluated. First, it was proved that colonisation of onion roots by <i>P. indica</i> was beneficial for plant growth, as it increased leaf development and root biomass. Most relevantly, <i>P. indica</i> was also effective in reducing Stemphylium leaf blight (SLB) severity, as assessed under greenhouse conditions and confirmed in field trials in two consecutive years. These investigations could also provide some insight into the biochemical and molecular changes that treatment with <i>P. indica</i> induces in the main pathways associated with host defence response. It was possible to highlight the protective effect of <i>P. indica</i> colonisation against peroxidative damage, and its role in signalling oxidative stress, by assessing changes in malondialdehyde and H<sub>2</sub>O<sub>2</sub> content. It was also showed that treatment with <i>P. indica</i> contributes to modulate the enzymatic activity of superoxide dismutase, catalase, phenylalanine ammonia-lyase and peroxidase, in the course of infection. qPCR-based expression analysis of defence-related genes <i>AcLOX1</i>, <i>AcLOX2</i>, <i>AcPAL1</i>, <i>AcGST</i>, <i>AcCHI</i>, <i>AcWRKY1,</i> and <i>AcWRKY70</i> provided further indications on <i>P. indica</i> ability to induce onion systemic response. Based on the evidence gathered, this study aims to propose <i>P. indica</i> application as a sustainable tool for improving SLB control, which might not only enhance onion growth performance but also activate defence signalling mechanisms more effectively, involving different pathways.https://www.mdpi.com/2076-0817/10/9/1085biocontrolInduced systemic resistanceonion<i>Piriformospora indica</i>Stemphylium leaf blight disease<i>Stemphylium vesicarium</i> |
| spellingShingle | Praveen Roylawar Kiran Khandagale Pragati Randive Bharat Shinde Chandrashekhar Murumkar Avinash Ade Major Singh Suresh Gawande Massimiliano Morelli <i>Piriformospora indica</i> Primes Onion Response against Stemphylium Leaf Blight Disease Pathogens biocontrol Induced systemic resistance onion <i>Piriformospora indica</i> Stemphylium leaf blight disease <i>Stemphylium vesicarium</i> |
| title | <i>Piriformospora indica</i> Primes Onion Response against Stemphylium Leaf Blight Disease |
| title_full | <i>Piriformospora indica</i> Primes Onion Response against Stemphylium Leaf Blight Disease |
| title_fullStr | <i>Piriformospora indica</i> Primes Onion Response against Stemphylium Leaf Blight Disease |
| title_full_unstemmed | <i>Piriformospora indica</i> Primes Onion Response against Stemphylium Leaf Blight Disease |
| title_short | <i>Piriformospora indica</i> Primes Onion Response against Stemphylium Leaf Blight Disease |
| title_sort | i piriformospora indica i primes onion response against stemphylium leaf blight disease |
| topic | biocontrol Induced systemic resistance onion <i>Piriformospora indica</i> Stemphylium leaf blight disease <i>Stemphylium vesicarium</i> |
| url | https://www.mdpi.com/2076-0817/10/9/1085 |
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