Decontamination of Titanium Surface Using Different Methods: An In Vitro Study
Contamination of implants is inevitable during different steps of production as well as during the clinical use. We devised a new implant cleaning strategy to restore the bioactivities on dental implant surfaces. We evaluated the efficiency of the Finevo cleaning system, and Ultraviolet and Plasma t...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Materials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1996-1944/13/10/2287 |
| _version_ | 1797567918982037504 |
|---|---|
| author | Sifan Yan Min Li Satoshi Komasa Akinori Agariguchi Yuanyuan Yang Yuhao Zeng Seiji Takao Honghao Zhang Yuichiro Tashiro Tetsuji Kusumoto Yasuyuki Kobayashi Liji Chen Kosuke Kashiwagi Naoyuki Matsumoto Joji Okazaki Takayoshi Kawazoe |
| author_facet | Sifan Yan Min Li Satoshi Komasa Akinori Agariguchi Yuanyuan Yang Yuhao Zeng Seiji Takao Honghao Zhang Yuichiro Tashiro Tetsuji Kusumoto Yasuyuki Kobayashi Liji Chen Kosuke Kashiwagi Naoyuki Matsumoto Joji Okazaki Takayoshi Kawazoe |
| author_sort | Sifan Yan |
| collection | DOAJ |
| description | Contamination of implants is inevitable during different steps of production as well as during the clinical use. We devised a new implant cleaning strategy to restore the bioactivities on dental implant surfaces. We evaluated the efficiency of the Finevo cleaning system, and Ultraviolet and Plasma treatments to decontaminate hydrocarbon-contaminated titanium disks. The surfaces of the contaminated titanium disks cleaned using the Finevo cleaning system were similar to those of the uncontaminated titanium disks in scanning electron microscopy and X-ray photoelectron spectroscopy analysis, but no obvious change in the roughness was observed in the scanning probe microscopy analysis. The rat bone marrow mesenchymal stem cells (rBMMSCs) cultured on the treated titanium disks attached to and covered the surfaces of disks cleaned with the Finevo cleaning system. The alkaline phosphatase activity, calcium deposition, and osteogenesis-related gene expression in rBMMSCs on disks cleaned using the Finevo cleaning system were higher compared to those in the ultraviolet and plasma treatments, displaying better cell functionality. Thus, the Finevo cleaning system can enhance the attachment, differentiation, and mineralization of rBMMSCs on treated titanium disk surfaces. This research provides a new strategy for cleaning the surface of contaminated titanium dental implants and for restoration of their biological functions. |
| first_indexed | 2024-03-10T19:48:59Z |
| format | Article |
| id | doaj.art-ccdc24954e214d8ca4a9d3e6e9808e4a |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T19:48:59Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-ccdc24954e214d8ca4a9d3e6e9808e4a2023-11-20T00:35:53ZengMDPI AGMaterials1996-19442020-05-011310228710.3390/ma13102287Decontamination of Titanium Surface Using Different Methods: An In Vitro StudySifan Yan0Min Li1Satoshi Komasa2Akinori Agariguchi3Yuanyuan Yang4Yuhao Zeng5Seiji Takao6Honghao Zhang7Yuichiro Tashiro8Tetsuji Kusumoto9Yasuyuki Kobayashi10Liji Chen11Kosuke Kashiwagi12Naoyuki Matsumoto13Joji Okazaki14Takayoshi Kawazoe15Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Oral Health Engineering, Faculty of Health Sciences, Osaka Dental University, Osaka 573-1121, JapanOsaka Research Institute of Industrial Science and Technology Morinomiya Center, 1-6-50, Morinomiya, Joto-ku, Osaka-shi 536-8553, JapanDepartment of Orthodntics, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Fixed Prosthodontics, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Orthodntics, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Fixed Prosthodontics, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanContamination of implants is inevitable during different steps of production as well as during the clinical use. We devised a new implant cleaning strategy to restore the bioactivities on dental implant surfaces. We evaluated the efficiency of the Finevo cleaning system, and Ultraviolet and Plasma treatments to decontaminate hydrocarbon-contaminated titanium disks. The surfaces of the contaminated titanium disks cleaned using the Finevo cleaning system were similar to those of the uncontaminated titanium disks in scanning electron microscopy and X-ray photoelectron spectroscopy analysis, but no obvious change in the roughness was observed in the scanning probe microscopy analysis. The rat bone marrow mesenchymal stem cells (rBMMSCs) cultured on the treated titanium disks attached to and covered the surfaces of disks cleaned with the Finevo cleaning system. The alkaline phosphatase activity, calcium deposition, and osteogenesis-related gene expression in rBMMSCs on disks cleaned using the Finevo cleaning system were higher compared to those in the ultraviolet and plasma treatments, displaying better cell functionality. Thus, the Finevo cleaning system can enhance the attachment, differentiation, and mineralization of rBMMSCs on treated titanium disk surfaces. This research provides a new strategy for cleaning the surface of contaminated titanium dental implants and for restoration of their biological functions.https://www.mdpi.com/1996-1944/13/10/2287Ti implanthydrocarbon decontaminationFinevo cleaning systemultraviolet treatmentplasma |
| spellingShingle | Sifan Yan Min Li Satoshi Komasa Akinori Agariguchi Yuanyuan Yang Yuhao Zeng Seiji Takao Honghao Zhang Yuichiro Tashiro Tetsuji Kusumoto Yasuyuki Kobayashi Liji Chen Kosuke Kashiwagi Naoyuki Matsumoto Joji Okazaki Takayoshi Kawazoe Decontamination of Titanium Surface Using Different Methods: An In Vitro Study Materials Ti implant hydrocarbon decontamination Finevo cleaning system ultraviolet treatment plasma |
| title | Decontamination of Titanium Surface Using Different Methods: An In Vitro Study |
| title_full | Decontamination of Titanium Surface Using Different Methods: An In Vitro Study |
| title_fullStr | Decontamination of Titanium Surface Using Different Methods: An In Vitro Study |
| title_full_unstemmed | Decontamination of Titanium Surface Using Different Methods: An In Vitro Study |
| title_short | Decontamination of Titanium Surface Using Different Methods: An In Vitro Study |
| title_sort | decontamination of titanium surface using different methods an in vitro study |
| topic | Ti implant hydrocarbon decontamination Finevo cleaning system ultraviolet treatment plasma |
| url | https://www.mdpi.com/1996-1944/13/10/2287 |
| work_keys_str_mv | AT sifanyan decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT minli decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT satoshikomasa decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT akinoriagariguchi decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT yuanyuanyang decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT yuhaozeng decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT seijitakao decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT honghaozhang decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT yuichirotashiro decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT tetsujikusumoto decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT yasuyukikobayashi decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT lijichen decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT kosukekashiwagi decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT naoyukimatsumoto decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT jojiokazaki decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy AT takayoshikawazoe decontaminationoftitaniumsurfaceusingdifferentmethodsaninvitrostudy |