Quantitative Determination of Aflatoxin by High Performance Liquid Chromatography in Wheat Silos in Golestan Province, North of Iran
Background: Aflatoxins are the most common mycotoxins that contaminate crops. They are produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. Wheat (Tricitumaestivum) is one of the most important staple foods used in Iran, and the environmental conditions in the north of Iran are...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-08-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/7323 |
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| author | Mohadeseh NAMJOO Faezeh SALAMAT Niloofar RAJABLI Reza HAJIHOSEEINI Farhad NIKNEJAD Faramarz KOHSAR Hamidreza JOSHAGHANI |
| author_facet | Mohadeseh NAMJOO Faezeh SALAMAT Niloofar RAJABLI Reza HAJIHOSEEINI Farhad NIKNEJAD Faramarz KOHSAR Hamidreza JOSHAGHANI |
| author_sort | Mohadeseh NAMJOO |
| collection | DOAJ |
| description | Background: Aflatoxins are the most common mycotoxins that contaminate crops. They are produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. Wheat (Tricitumaestivum) is one of the most important staple foods used in Iran, and the environmental conditions in the north of Iran are favorable to fungal growth. This study was designed in order to determine the aflatoxin concentration in wheat samples from silos in Golestan Province north of Iran.
Methods: Samples were collected from three silos of Golestan province. First, aflatoxins were isolated using immunoaffinity chromatography. Then the aflatoxin concentrations were determined by High performance liquid chromatography (HPLC) method and fluorescence detector.
Results: Ten out of 34 samples (29.4% of samples) were contaminated by aflatoxins.No concentration was found above permitted aflatoxin levels in Iran (15 ng/g). In one sample (2.9%), aflatoxin B1 was seen over the permissible limits in Iran. The highest level found in samples for total aflatoxin, aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2 were 7.08 ng/g, 6.91 ng/g, 0.29 ng/g, 1.37 ng/g and 0.23 ng/g, respectively. No correlation was found between humidity levels in wheat samples contained aflatoxin and wheat samples without aflatoxin.
Conclusion: Despite the total aflatoxins determined in samples were below the permissible limits in Iran, the 29% aflatoxin contamination rate can negatively affect health factors and it should not be neglected. So, it is predictable that if the storage duration of samples increases, the aflatoxin contamination levels will increase. |
| first_indexed | 2024-04-11T14:41:01Z |
| format | Article |
| id | doaj.art-cce020c27119480ea514ad5194a0fc99 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-04-11T14:41:01Z |
| publishDate | 2016-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-cce020c27119480ea514ad5194a0fc992022-12-22T04:17:58ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932016-08-014574953Quantitative Determination of Aflatoxin by High Performance Liquid Chromatography in Wheat Silos in Golestan Province, North of IranMohadeseh NAMJOO0Faezeh SALAMAT1Niloofar RAJABLI2Reza HAJIHOSEEINI3Farhad NIKNEJAD4Faramarz KOHSAR5Hamidreza JOSHAGHANI6Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, IranGolestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, IranGolestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, IranDept. of Biochemistry, Payame Noor University, Tehran, IranLaboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, IranLaboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, IranGolestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, IranBackground: Aflatoxins are the most common mycotoxins that contaminate crops. They are produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. Wheat (Tricitumaestivum) is one of the most important staple foods used in Iran, and the environmental conditions in the north of Iran are favorable to fungal growth. This study was designed in order to determine the aflatoxin concentration in wheat samples from silos in Golestan Province north of Iran. Methods: Samples were collected from three silos of Golestan province. First, aflatoxins were isolated using immunoaffinity chromatography. Then the aflatoxin concentrations were determined by High performance liquid chromatography (HPLC) method and fluorescence detector. Results: Ten out of 34 samples (29.4% of samples) were contaminated by aflatoxins.No concentration was found above permitted aflatoxin levels in Iran (15 ng/g). In one sample (2.9%), aflatoxin B1 was seen over the permissible limits in Iran. The highest level found in samples for total aflatoxin, aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2 were 7.08 ng/g, 6.91 ng/g, 0.29 ng/g, 1.37 ng/g and 0.23 ng/g, respectively. No correlation was found between humidity levels in wheat samples contained aflatoxin and wheat samples without aflatoxin. Conclusion: Despite the total aflatoxins determined in samples were below the permissible limits in Iran, the 29% aflatoxin contamination rate can negatively affect health factors and it should not be neglected. So, it is predictable that if the storage duration of samples increases, the aflatoxin contamination levels will increase.https://ijph.tums.ac.ir/index.php/ijph/article/view/7323AflatoxinWheatHPLCIran |
| spellingShingle | Mohadeseh NAMJOO Faezeh SALAMAT Niloofar RAJABLI Reza HAJIHOSEEINI Farhad NIKNEJAD Faramarz KOHSAR Hamidreza JOSHAGHANI Quantitative Determination of Aflatoxin by High Performance Liquid Chromatography in Wheat Silos in Golestan Province, North of Iran Iranian Journal of Public Health Aflatoxin Wheat HPLC Iran |
| title | Quantitative Determination of Aflatoxin by High Performance Liquid Chromatography in Wheat Silos in Golestan Province, North of Iran |
| title_full | Quantitative Determination of Aflatoxin by High Performance Liquid Chromatography in Wheat Silos in Golestan Province, North of Iran |
| title_fullStr | Quantitative Determination of Aflatoxin by High Performance Liquid Chromatography in Wheat Silos in Golestan Province, North of Iran |
| title_full_unstemmed | Quantitative Determination of Aflatoxin by High Performance Liquid Chromatography in Wheat Silos in Golestan Province, North of Iran |
| title_short | Quantitative Determination of Aflatoxin by High Performance Liquid Chromatography in Wheat Silos in Golestan Province, North of Iran |
| title_sort | quantitative determination of aflatoxin by high performance liquid chromatography in wheat silos in golestan province north of iran |
| topic | Aflatoxin Wheat HPLC Iran |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/7323 |
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