Denaturation of α-lactalbumin and bovine serum albumin in pressure-treated reconstituted skim milk
The kinetics of α-lactalbumin and bovine serum albumin (BSA) denaturation in pressure-treated reconstituted skim milk samples at pressures of 100–800 MPa and temperatures of 10–40 °C was studied. No denaturation was observed at pressures up to 400 MPa, whereas at 500 MPa or higher, the level of dena...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Food Chemistry Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X21000022 |
| _version_ | 1811178663828258816 |
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| author | Skelte G. Anema |
| author_facet | Skelte G. Anema |
| author_sort | Skelte G. Anema |
| collection | DOAJ |
| description | The kinetics of α-lactalbumin and bovine serum albumin (BSA) denaturation in pressure-treated reconstituted skim milk samples at pressures of 100–800 MPa and temperatures of 10–40 °C was studied. No denaturation was observed at pressures up to 400 MPa, whereas at 500 MPa or higher, the level of denaturation increased with increasing holding time at each pressure/temperature, with increasing pressure at each holding time/temperature or with temperature at each pressure/holding time. BSA denatured at a faster rate than α-lactalbumin, and denaturation for both proteins could be described as second order. Evaluation of the kinetic and thermodynamic parameters suggested that α-lactalbumin denaturation was an aggregation-limited reaction, whereas for BSA, the denaturation transitioned from aggregation-limited to unfolding-limited as the pressure increased. |
| first_indexed | 2024-04-11T06:22:13Z |
| format | Article |
| id | doaj.art-cce07ada4f284038904757829e23745f |
| institution | Directory Open Access Journal |
| issn | 2772-753X |
| language | English |
| last_indexed | 2024-04-11T06:22:13Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj.art-cce07ada4f284038904757829e23745f2022-12-22T04:40:32ZengElsevierFood Chemistry Advances2772-753X2022-10-011100002Denaturation of α-lactalbumin and bovine serum albumin in pressure-treated reconstituted skim milkSkelte G. Anema0Fonterra Research and Development Centre, Palmerston North, New Zealand; School of Food and Advanced Technology, Massey University, Private Bag 11 222, Palmerston North, New Zealand; Riddet Institute, Massey University, Private Bag 11 222, Palmerston North, New ZealandThe kinetics of α-lactalbumin and bovine serum albumin (BSA) denaturation in pressure-treated reconstituted skim milk samples at pressures of 100–800 MPa and temperatures of 10–40 °C was studied. No denaturation was observed at pressures up to 400 MPa, whereas at 500 MPa or higher, the level of denaturation increased with increasing holding time at each pressure/temperature, with increasing pressure at each holding time/temperature or with temperature at each pressure/holding time. BSA denatured at a faster rate than α-lactalbumin, and denaturation for both proteins could be described as second order. Evaluation of the kinetic and thermodynamic parameters suggested that α-lactalbumin denaturation was an aggregation-limited reaction, whereas for BSA, the denaturation transitioned from aggregation-limited to unfolding-limited as the pressure increased.http://www.sciencedirect.com/science/article/pii/S2772753X21000022α-lactalbuminBovine serum albuminMilkDenaturationHigh pressure |
| spellingShingle | Skelte G. Anema Denaturation of α-lactalbumin and bovine serum albumin in pressure-treated reconstituted skim milk Food Chemistry Advances α-lactalbumin Bovine serum albumin Milk Denaturation High pressure |
| title | Denaturation of α-lactalbumin and bovine serum albumin in pressure-treated reconstituted skim milk |
| title_full | Denaturation of α-lactalbumin and bovine serum albumin in pressure-treated reconstituted skim milk |
| title_fullStr | Denaturation of α-lactalbumin and bovine serum albumin in pressure-treated reconstituted skim milk |
| title_full_unstemmed | Denaturation of α-lactalbumin and bovine serum albumin in pressure-treated reconstituted skim milk |
| title_short | Denaturation of α-lactalbumin and bovine serum albumin in pressure-treated reconstituted skim milk |
| title_sort | denaturation of α lactalbumin and bovine serum albumin in pressure treated reconstituted skim milk |
| topic | α-lactalbumin Bovine serum albumin Milk Denaturation High pressure |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X21000022 |
| work_keys_str_mv | AT skelteganema denaturationofalactalbuminandbovineserumalbumininpressuretreatedreconstitutedskimmilk |