Mitochondrial transplantation: Effects on chemotherapy in prostate and ovarian cancer cells in vitro and in vivo
Prostate and ovarian cancers affect the male and female reproductive organs and are among the most common cancers in developing countries. Previous studies have demonstrated that cancer cells have a high rate of aerobic glycolysis that is present in nearly all invasive human cancers and persists eve...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Biomedicine & Pharmacotherapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223003128 |
| _version_ | 1797857712388702208 |
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| author | Aybuke Celik Arzoo Orfany Jason Dearling Pedro J. del Nido James D. McCully Filiz Bakar-Ates |
| author_facet | Aybuke Celik Arzoo Orfany Jason Dearling Pedro J. del Nido James D. McCully Filiz Bakar-Ates |
| author_sort | Aybuke Celik |
| collection | DOAJ |
| description | Prostate and ovarian cancers affect the male and female reproductive organs and are among the most common cancers in developing countries. Previous studies have demonstrated that cancer cells have a high rate of aerobic glycolysis that is present in nearly all invasive human cancers and persists even under normoxic conditions. Aerobic glycolysis has been correlated with chemotherapeutic resistance and tumor aggressiveness. These data suggest that mitochondrial dysfunction may confer a significant proliferative advantage during the somatic evolution of cancer. In this study we investigated the effect of direct mitochondria transplantation on cancer cell proliferation and chemotherapeutic sensitivity in prostate and ovarian cancer models, both in vitro and in vivo. Our results show that the transplantation of viable, respiration competent mitochondria has no effect on cancer cell proliferation but significantly decreases migration and alters cell cycle checkpoints. Our results further demonstrate that mitochondrial transplantation significantly increases chemotherapeutic sensitivity, providing similar apoptotic levels with low-dose chemotherapy as that achieved with high-dose chemotherapy. These results suggest that mitochondria transplantation provides a novel approach for early prostate and ovarian cancer therapy, significantly increasing chemotherapeutic sensitivity in in vitro and in vivo murine models. |
| first_indexed | 2024-04-09T21:01:13Z |
| format | Article |
| id | doaj.art-cce0aa03ba1944a8a3a23f1a1a7ab412 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-09T21:01:13Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-cce0aa03ba1944a8a3a23f1a1a7ab4122023-03-29T09:23:45ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-05-01161114524Mitochondrial transplantation: Effects on chemotherapy in prostate and ovarian cancer cells in vitro and in vivoAybuke Celik0Arzoo Orfany1Jason Dearling2Pedro J. del Nido3James D. McCully4Filiz Bakar-Ates5Department of Biochemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey; Department of Cardiac Surgery, Boston Children’s Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USADepartment of Cardiac Surgery, Boston Children’s Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USADepartment of Radiology, Boston Children’s Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USADepartment of Cardiac Surgery, Boston Children’s Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USADepartment of Cardiac Surgery, Boston Children’s Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USADepartment of Biochemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey; Corresponding author.Prostate and ovarian cancers affect the male and female reproductive organs and are among the most common cancers in developing countries. Previous studies have demonstrated that cancer cells have a high rate of aerobic glycolysis that is present in nearly all invasive human cancers and persists even under normoxic conditions. Aerobic glycolysis has been correlated with chemotherapeutic resistance and tumor aggressiveness. These data suggest that mitochondrial dysfunction may confer a significant proliferative advantage during the somatic evolution of cancer. In this study we investigated the effect of direct mitochondria transplantation on cancer cell proliferation and chemotherapeutic sensitivity in prostate and ovarian cancer models, both in vitro and in vivo. Our results show that the transplantation of viable, respiration competent mitochondria has no effect on cancer cell proliferation but significantly decreases migration and alters cell cycle checkpoints. Our results further demonstrate that mitochondrial transplantation significantly increases chemotherapeutic sensitivity, providing similar apoptotic levels with low-dose chemotherapy as that achieved with high-dose chemotherapy. These results suggest that mitochondria transplantation provides a novel approach for early prostate and ovarian cancer therapy, significantly increasing chemotherapeutic sensitivity in in vitro and in vivo murine models.http://www.sciencedirect.com/science/article/pii/S0753332223003128Mitochondrial transplantationProstate cancerOvarian cancerCisplatinDocetaxelPaclitaxel |
| spellingShingle | Aybuke Celik Arzoo Orfany Jason Dearling Pedro J. del Nido James D. McCully Filiz Bakar-Ates Mitochondrial transplantation: Effects on chemotherapy in prostate and ovarian cancer cells in vitro and in vivo Biomedicine & Pharmacotherapy Mitochondrial transplantation Prostate cancer Ovarian cancer Cisplatin Docetaxel Paclitaxel |
| title | Mitochondrial transplantation: Effects on chemotherapy in prostate and ovarian cancer cells in vitro and in vivo |
| title_full | Mitochondrial transplantation: Effects on chemotherapy in prostate and ovarian cancer cells in vitro and in vivo |
| title_fullStr | Mitochondrial transplantation: Effects on chemotherapy in prostate and ovarian cancer cells in vitro and in vivo |
| title_full_unstemmed | Mitochondrial transplantation: Effects on chemotherapy in prostate and ovarian cancer cells in vitro and in vivo |
| title_short | Mitochondrial transplantation: Effects on chemotherapy in prostate and ovarian cancer cells in vitro and in vivo |
| title_sort | mitochondrial transplantation effects on chemotherapy in prostate and ovarian cancer cells in vitro and in vivo |
| topic | Mitochondrial transplantation Prostate cancer Ovarian cancer Cisplatin Docetaxel Paclitaxel |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223003128 |
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