Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa.
Selenium (Se) has been becoming an emerging pollutant causing severe phytotoxicity, which the biochemical mechanism is rarely known. Although hydrogen sulfide (H2S) has been suggested as an important exogenous regulator modulating plant physiological adaptions in response to heavy metal stress, whet...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4204939?pdf=render |
| _version_ | 1819111260172582912 |
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| author | Yi Chen Hai-Zhen Mo Mei-Yu Zheng Ming Xian Zhong-Qiang Qi You-Qin Li Liang-Bin Hu Jian Chen Li-Fei Yang |
| author_facet | Yi Chen Hai-Zhen Mo Mei-Yu Zheng Ming Xian Zhong-Qiang Qi You-Qin Li Liang-Bin Hu Jian Chen Li-Fei Yang |
| author_sort | Yi Chen |
| collection | DOAJ |
| description | Selenium (Se) has been becoming an emerging pollutant causing severe phytotoxicity, which the biochemical mechanism is rarely known. Although hydrogen sulfide (H2S) has been suggested as an important exogenous regulator modulating plant physiological adaptions in response to heavy metal stress, whether and how the endogenous H2S regulates Se-induce phytotoxicity remains unclear. In this work, a self-developed specific fluorescent probe (WSP-1) was applied to track endogenous H2S in situ in the roots of Brassica rapa under Se(IV) stress. Se(IV)-induced root growth stunt was closely correlated with the inhibition of endogenous H2S generation in root tips. Se(IV) stress dampened the expression of most LCD and DCD homologues in the roots of B. rapa. By using various specific fluorescent probes for bio-imaging root tips in situ, we found that the increase in endogenous H2S by the application of H2S donor NaHS could significantly alleviate Se(IV)-induced reactive oxygen species (ROS) over-accumulation, oxidative impairment, and cell death in root tips, which further resulted in the recovery of root growth under Se(IV) stress. However, dampening the endogenous H2S could block the alleviated effect of NaHS on Se(IV)-induced phytotoxicity. Finally, the increase in endogenous H2S resulted in the enhancement of glutathione (GSH) in Se(IV)-treated roots, which may share the similar molecular mechanism for the dominant role of H2S in removing ROS by activating GSH biosynthesis in mammals. Altogether, these data provide the first direct evidences confirming the pivotal role of endogenous H2S in modulating Se(IV)-induced phytotoxicity in roots. |
| first_indexed | 2024-12-22T03:54:47Z |
| format | Article |
| id | doaj.art-cce2ea9ae895462c99142cfce8a7d2ff |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T03:54:47Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cce2ea9ae895462c99142cfce8a7d2ff2022-12-21T18:39:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e11090410.1371/journal.pone.0110904Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa.Yi ChenHai-Zhen MoMei-Yu ZhengMing XianZhong-Qiang QiYou-Qin LiLiang-Bin HuJian ChenLi-Fei YangSelenium (Se) has been becoming an emerging pollutant causing severe phytotoxicity, which the biochemical mechanism is rarely known. Although hydrogen sulfide (H2S) has been suggested as an important exogenous regulator modulating plant physiological adaptions in response to heavy metal stress, whether and how the endogenous H2S regulates Se-induce phytotoxicity remains unclear. In this work, a self-developed specific fluorescent probe (WSP-1) was applied to track endogenous H2S in situ in the roots of Brassica rapa under Se(IV) stress. Se(IV)-induced root growth stunt was closely correlated with the inhibition of endogenous H2S generation in root tips. Se(IV) stress dampened the expression of most LCD and DCD homologues in the roots of B. rapa. By using various specific fluorescent probes for bio-imaging root tips in situ, we found that the increase in endogenous H2S by the application of H2S donor NaHS could significantly alleviate Se(IV)-induced reactive oxygen species (ROS) over-accumulation, oxidative impairment, and cell death in root tips, which further resulted in the recovery of root growth under Se(IV) stress. However, dampening the endogenous H2S could block the alleviated effect of NaHS on Se(IV)-induced phytotoxicity. Finally, the increase in endogenous H2S resulted in the enhancement of glutathione (GSH) in Se(IV)-treated roots, which may share the similar molecular mechanism for the dominant role of H2S in removing ROS by activating GSH biosynthesis in mammals. Altogether, these data provide the first direct evidences confirming the pivotal role of endogenous H2S in modulating Se(IV)-induced phytotoxicity in roots.http://europepmc.org/articles/PMC4204939?pdf=render |
| spellingShingle | Yi Chen Hai-Zhen Mo Mei-Yu Zheng Ming Xian Zhong-Qiang Qi You-Qin Li Liang-Bin Hu Jian Chen Li-Fei Yang Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa. PLoS ONE |
| title | Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa. |
| title_full | Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa. |
| title_fullStr | Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa. |
| title_full_unstemmed | Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa. |
| title_short | Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa. |
| title_sort | selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in brassica rapa |
| url | http://europepmc.org/articles/PMC4204939?pdf=render |
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