Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid
Abstract Hepatocellular carcinoma (HCC) treatment remains lack of effective chemopreventive agents, therefore it is very attractive and urgent to discover novel anti-HCC drugs. In the present study, the effects of chlorogenic acid (ChA) and caffeic acid (CaA) on HCC induced by diethylnitrosamine (DE...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-04888-y |
| _version_ | 1818839900297887744 |
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| author | Zhan Zhang Di Wang Shanlei Qiao Xinyue Wu Shuyuan Cao Li Wang Xiaojian Su Lei Li |
| author_facet | Zhan Zhang Di Wang Shanlei Qiao Xinyue Wu Shuyuan Cao Li Wang Xiaojian Su Lei Li |
| author_sort | Zhan Zhang |
| collection | DOAJ |
| description | Abstract Hepatocellular carcinoma (HCC) treatment remains lack of effective chemopreventive agents, therefore it is very attractive and urgent to discover novel anti-HCC drugs. In the present study, the effects of chlorogenic acid (ChA) and caffeic acid (CaA) on HCC induced by diethylnitrosamine (DEN) were evaluated. ChA or CaA could reduce the histopathological changes and liver injury markers, such as alanine transarninase, aspartate aminotransferase, alkaline phosphatase, total bile acid, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol. The underlying mechanisms were investigated by a data integration strategy based on correlation analyses of metabonomics data and 16 S rRNA gene sequencing data. ChA or CaA could inhibit the increase of Rumincoccaceae UCG-004 and reduction of Lachnospiraceae incertae sedis, and Prevotella 9 in HCC rats. The principal component analysis and partial least squares discriminant analysis were applied to reveal the metabolic differences among these groups. 28 different metabolites showed a trend to return to normal in both CaA and ChA treatment. Among them, Bilirubin, L-Tyrosine, L-Methionine and Ethanolamine were correlated increased Rumincoccaceae UCG-004 and decreased of Lachnospiraceae incertae sedis and Prevotella 9. These correlations could be identified as metabolic and microbial signatures of HCC onset and potential therapeutic targets. |
| first_indexed | 2024-12-19T04:01:38Z |
| format | Article |
| id | doaj.art-cce35029c8e040e9ac0b92902dd2de46 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:01:38Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-cce35029c8e040e9ac0b92902dd2de462022-12-21T20:36:40ZengNature PortfolioScientific Reports2045-23222017-07-017111010.1038/s41598-017-04888-yMetabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acidZhan Zhang0Di Wang1Shanlei Qiao2Xinyue Wu3Shuyuan Cao4Li Wang5Xiaojian Su6Lei Li7Department of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical UniversityDepartment of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical UniversityDepartment of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical UniversityDepartment of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical UniversityDepartment of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical UniversityDepartment of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical UniversityNanjing entry-exit inspection and quarantine bureauDepartment of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical UniversityAbstract Hepatocellular carcinoma (HCC) treatment remains lack of effective chemopreventive agents, therefore it is very attractive and urgent to discover novel anti-HCC drugs. In the present study, the effects of chlorogenic acid (ChA) and caffeic acid (CaA) on HCC induced by diethylnitrosamine (DEN) were evaluated. ChA or CaA could reduce the histopathological changes and liver injury markers, such as alanine transarninase, aspartate aminotransferase, alkaline phosphatase, total bile acid, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol. The underlying mechanisms were investigated by a data integration strategy based on correlation analyses of metabonomics data and 16 S rRNA gene sequencing data. ChA or CaA could inhibit the increase of Rumincoccaceae UCG-004 and reduction of Lachnospiraceae incertae sedis, and Prevotella 9 in HCC rats. The principal component analysis and partial least squares discriminant analysis were applied to reveal the metabolic differences among these groups. 28 different metabolites showed a trend to return to normal in both CaA and ChA treatment. Among them, Bilirubin, L-Tyrosine, L-Methionine and Ethanolamine were correlated increased Rumincoccaceae UCG-004 and decreased of Lachnospiraceae incertae sedis and Prevotella 9. These correlations could be identified as metabolic and microbial signatures of HCC onset and potential therapeutic targets.https://doi.org/10.1038/s41598-017-04888-y |
| spellingShingle | Zhan Zhang Di Wang Shanlei Qiao Xinyue Wu Shuyuan Cao Li Wang Xiaojian Su Lei Li Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid Scientific Reports |
| title | Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid |
| title_full | Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid |
| title_fullStr | Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid |
| title_full_unstemmed | Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid |
| title_short | Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid |
| title_sort | metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid |
| url | https://doi.org/10.1038/s41598-017-04888-y |
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