Minocycline-Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan-Induced Diabetic Mice
Diabetes is a life-threatening disease, and chronic diabetes affects parts of the body including the liver, kidney, and pancreas. The root cause of diabetes is mainly associated with oxidative stress produced by reactive oxygen species. Minocycline is a drug with a multi-substituted phenol ring and...
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| Aineistotyyppi: | Artikkeli |
| Kieli: | English |
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MDPI AG
2021-10-01
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| Sarja: | Pharmaceutics |
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| Linkit: | https://www.mdpi.com/1999-4923/13/10/1678 |
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| author | Syed Akif Raza Kazmi Muhammad Zahid Qureshi Sadia Saleh S. Alhewairini Shaukat Ali Shazia Khurshid Muhammad Saeed Shumaila Mumtaz Tafail Akbar Mughal |
| author_facet | Syed Akif Raza Kazmi Muhammad Zahid Qureshi Sadia Saleh S. Alhewairini Shaukat Ali Shazia Khurshid Muhammad Saeed Shumaila Mumtaz Tafail Akbar Mughal |
| author_sort | Syed Akif Raza Kazmi |
| collection | DOAJ |
| description | Diabetes is a life-threatening disease, and chronic diabetes affects parts of the body including the liver, kidney, and pancreas. The root cause of diabetes is mainly associated with oxidative stress produced by reactive oxygen species. Minocycline is a drug with a multi-substituted phenol ring and has shown excellent antioxidant activities. The objective of the present study was to investigate the antidiabetic potential of minocycline-modified silver nanoparticles (mino/AgNPs) against alloxan-induced diabetic mice. The mino/AgNPs were synthesized using minocycline as reducing and stabilizing agents. UV-visible, FT-IR, X-ray diffraction (XRD), and transmission electron microscopy (TEM) were applied for the characterization of mino/AgNPs. A 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay was conducted to determine the antioxidant potential of newly synthesized mino/AgNPs. The results revealed that the mino/AgNPs showed higher radical scavenging activity (IC<sub>50</sub> = 19.7 µg/mL) compared to the minocycline (IC<sub>50</sub> = 26.0 µg/mL) and ascorbic acid (IC<sub>50</sub> = 25.2 µg/mL). Further, mino/AgNPs were successfully employed to examine their antidiabetic potential against alloxan-induced diabetic mice. Hematological results showed that the mice treated with mino/AgNPs demonstrated a significant decrease in fasting blood glucose level and lipid profile compared to the untreated diabetic group. A histopathological examination confirmed that the diabetic mice treated with mino/AgNPs showed significant recovery and revival of the histo-morphology of the kidney, central vein of the liver, and islet cells of the pancreas compared to the untreated diabetic mice. Hence, mino/AgNPs have good antidiabetic potential and could be an appropriate nanomedicine to prevent the development of diabetes. |
| first_indexed | 2024-03-10T06:16:38Z |
| format | Article |
| id | doaj.art-cce76ed48d7844dc8f67b115dd1040e6 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T06:16:38Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-cce76ed48d7844dc8f67b115dd1040e62023-11-22T19:39:36ZengMDPI AGPharmaceutics1999-49232021-10-011310167810.3390/pharmaceutics13101678Minocycline-Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan-Induced Diabetic MiceSyed Akif Raza Kazmi0Muhammad Zahid Qureshi1Sadia2Saleh S. Alhewairini3Shaukat Ali4Shazia Khurshid5Muhammad Saeed6Shumaila Mumtaz7Tafail Akbar Mughal8Department of Chemistry, Government College University Lahore, Lahore 54000, PakistanDepartment of Chemistry, Government College University Lahore, Lahore 54000, PakistanDepartment of Biotechnology, University of Azad Jammu and Kashmir, Muzaffarabad 13100, PakistanDepartment of Plant Production and Protection, College of Agriculture and Veterinary Medicine, Qassim University, Buraidah 51452, Saudi ArabiaMedical Toxicology Laboratory, Department of Zoology, Government College University Lahore, Lahore 54000, PakistanDepartment of Chemistry, Government College University Lahore, Lahore 54000, PakistanDepartment of Chemistry and Chemical Engineering, SBASSE LUMS, Lahore 54692, PakistanMedical Toxicology Laboratory, Department of Zoology, Government College University Lahore, Lahore 54000, PakistanMedical Toxicology Laboratory, Department of Zoology, Government College University Lahore, Lahore 54000, PakistanDiabetes is a life-threatening disease, and chronic diabetes affects parts of the body including the liver, kidney, and pancreas. The root cause of diabetes is mainly associated with oxidative stress produced by reactive oxygen species. Minocycline is a drug with a multi-substituted phenol ring and has shown excellent antioxidant activities. The objective of the present study was to investigate the antidiabetic potential of minocycline-modified silver nanoparticles (mino/AgNPs) against alloxan-induced diabetic mice. The mino/AgNPs were synthesized using minocycline as reducing and stabilizing agents. UV-visible, FT-IR, X-ray diffraction (XRD), and transmission electron microscopy (TEM) were applied for the characterization of mino/AgNPs. A 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay was conducted to determine the antioxidant potential of newly synthesized mino/AgNPs. The results revealed that the mino/AgNPs showed higher radical scavenging activity (IC<sub>50</sub> = 19.7 µg/mL) compared to the minocycline (IC<sub>50</sub> = 26.0 µg/mL) and ascorbic acid (IC<sub>50</sub> = 25.2 µg/mL). Further, mino/AgNPs were successfully employed to examine their antidiabetic potential against alloxan-induced diabetic mice. Hematological results showed that the mice treated with mino/AgNPs demonstrated a significant decrease in fasting blood glucose level and lipid profile compared to the untreated diabetic group. A histopathological examination confirmed that the diabetic mice treated with mino/AgNPs showed significant recovery and revival of the histo-morphology of the kidney, central vein of the liver, and islet cells of the pancreas compared to the untreated diabetic mice. Hence, mino/AgNPs have good antidiabetic potential and could be an appropriate nanomedicine to prevent the development of diabetes.https://www.mdpi.com/1999-4923/13/10/1678minocyclinesilver nanoparticlestetracyclineantidiabeticin vivonanomedicine |
| spellingShingle | Syed Akif Raza Kazmi Muhammad Zahid Qureshi Sadia Saleh S. Alhewairini Shaukat Ali Shazia Khurshid Muhammad Saeed Shumaila Mumtaz Tafail Akbar Mughal Minocycline-Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan-Induced Diabetic Mice Pharmaceutics minocycline silver nanoparticles tetracycline antidiabetic in vivo nanomedicine |
| title | Minocycline-Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan-Induced Diabetic Mice |
| title_full | Minocycline-Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan-Induced Diabetic Mice |
| title_fullStr | Minocycline-Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan-Induced Diabetic Mice |
| title_full_unstemmed | Minocycline-Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan-Induced Diabetic Mice |
| title_short | Minocycline-Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan-Induced Diabetic Mice |
| title_sort | minocycline derived silver nanoparticles for assessment of their antidiabetic potential against alloxan induced diabetic mice |
| topic | minocycline silver nanoparticles tetracycline antidiabetic in vivo nanomedicine |
| url | https://www.mdpi.com/1999-4923/13/10/1678 |
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