The effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes

Background: Heat shock protein 60 (HSP60) is considered as an indicator of mitochondrial stress and plays a role in modulating mitochondrial dysfunction. This study aimed to investigate the effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with t...

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Main Authors: Mehrzad Shabani, Vahid Valipour-Dehnou, Mohammad Reza Tabandeh, Mahdieh Molanouri-Shamsi
Format: Article
Language:fas
Published: Kashan University of Medical Sciences and Health Services 2022-08-01
Series:Fiyz̤
Subjects:
Online Access:http://feyz.kaums.ac.ir/article-1-4588-en.html
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author Mehrzad Shabani
Vahid Valipour-Dehnou
Mohammad Reza Tabandeh
Mahdieh Molanouri-Shamsi
author_facet Mehrzad Shabani
Vahid Valipour-Dehnou
Mohammad Reza Tabandeh
Mahdieh Molanouri-Shamsi
author_sort Mehrzad Shabani
collection DOAJ
description Background: Heat shock protein 60 (HSP60) is considered as an indicator of mitochondrial stress and plays a role in modulating mitochondrial dysfunction. This study aimed to investigate the effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes. Materials and Methods: Thirty adult mice were randomly and equally divided into three groups: control (C), diabetic (D) and diabetic-exercise (DE). Diabetes was induced by intraperitoneal injection of Streptozotocin. Aerobic endurance training was performed five sessions per week for eight weeks. 48 hours after the last training session, glucose, insulin and HSP60 levels and insulin resistance index were measured. Results: Insulin and blood glucose levels and insulin resistance in group DE decreased significantly compared to group D (P<0.05). Also, HSP60 protein levels in group DE increased significantly compared to group D (P<0.05) and decreased significantly in group D compared to group C (P<0.05). Also, the results showed that there was a negative correlation between HSP60 and insulin resistance in all three groups (P= 0.001, r= 0.930). Conclusion: Diabetes lowers HSP60 levels and aerobic endurance exercise increases its levels. Also, endurance exercise can enhance mitochondrial function in diabetes by increasing HSP60 protein expression. It improves the insulin resistance index, also.
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spelling doaj.art-ccecc55e1c5e42eeb93b5da7953510192022-12-22T01:36:03ZfasKashan University of Medical Sciences and Health ServicesFiyz̤1029-78552008-98212022-08-01263273281 The effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetesMehrzad Shabani 0Vahid Valipour-Dehnou1Mohammad Reza Tabandeh2Mahdieh Molanouri-Shamsi3Department of Sport Sciences, Faculty of Literature and Human Sciences, Lorestan University, Khorramabad, I.R. Iran.Department of Sport Sciences, Faculty of Literature and Human Sciences, Lorestan University, Khorramabad, I.R. Iran.Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, I.R. Iran.Department of Physical Education and Sport Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, I.R. Iran.Background: Heat shock protein 60 (HSP60) is considered as an indicator of mitochondrial stress and plays a role in modulating mitochondrial dysfunction. This study aimed to investigate the effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes. Materials and Methods: Thirty adult mice were randomly and equally divided into three groups: control (C), diabetic (D) and diabetic-exercise (DE). Diabetes was induced by intraperitoneal injection of Streptozotocin. Aerobic endurance training was performed five sessions per week for eight weeks. 48 hours after the last training session, glucose, insulin and HSP60 levels and insulin resistance index were measured. Results: Insulin and blood glucose levels and insulin resistance in group DE decreased significantly compared to group D (P<0.05). Also, HSP60 protein levels in group DE increased significantly compared to group D (P<0.05) and decreased significantly in group D compared to group C (P<0.05). Also, the results showed that there was a negative correlation between HSP60 and insulin resistance in all three groups (P= 0.001, r= 0.930). Conclusion: Diabetes lowers HSP60 levels and aerobic endurance exercise increases its levels. Also, endurance exercise can enhance mitochondrial function in diabetes by increasing HSP60 protein expression. It improves the insulin resistance index, also.http://feyz.kaums.ac.ir/article-1-4588-en.htmlaerobic exercisediabetesinsulin resistancehsp60mice
spellingShingle Mehrzad Shabani
Vahid Valipour-Dehnou
Mohammad Reza Tabandeh
Mahdieh Molanouri-Shamsi
The effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes
Fiyz̤
aerobic exercise
diabetes
insulin resistance
hsp60
mice
title The effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes
title_full The effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes
title_fullStr The effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes
title_full_unstemmed The effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes
title_short The effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes
title_sort effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes
topic aerobic exercise
diabetes
insulin resistance
hsp60
mice
url http://feyz.kaums.ac.ir/article-1-4588-en.html
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