Deeper Insights into the Effect of Humic Acid on Kitchen Waste Anaerobic Digestion: Enzyme Activities, Microbial Community Dynamics, and Key Metabolic Pathways
Anaerobic digestion (AD) represents one of the most eco-friendly approaches for recovering the energy from kitchen waste (KW). This study investigated the impact of humic acid (HA) on AD of KW. Batch experiments were conducted using KW as the substrate, with varying HA dosages. The results revealed...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/10/881 |
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| author | Lin Lyu Yanzeng Li Shenghua Zhang Zhou Chen |
| author_facet | Lin Lyu Yanzeng Li Shenghua Zhang Zhou Chen |
| author_sort | Lin Lyu |
| collection | DOAJ |
| description | Anaerobic digestion (AD) represents one of the most eco-friendly approaches for recovering the energy from kitchen waste (KW). This study investigated the impact of humic acid (HA) on AD of KW. Batch experiments were conducted using KW as the substrate, with varying HA dosages. The results revealed that an increase in HA dosage led to an inhibition of methanogenic efficiency by 29.51% and a delayed start-up of AD. The HA exhibited dual effects on enzyme activities during AD, inhibiting hydrolytic enzymes while stimulating acidogenic enzymes. This unique interaction ultimately resulted in a significant 12.32% increase in volatile fatty acid production. Furthermore, HA induced the generation of reactive oxygen species and had a discernible impact on the activity of the electron transfer system. A bioinformatics analysis highlighted that HA promoted the abundance of microorganisms with mediated interspecies electron transfer ability, including DMER64 and Methanosaeta, as well as the Firmicutes phylum, while significantly reducing the abundance of Methanobacterium. Moreover, the KEGG pathway analysis revealed a decrease in hydrolysis and methanogenesis-related genes and an increase in acidogenesis-related genes. |
| first_indexed | 2024-03-10T21:16:02Z |
| format | Article |
| id | doaj.art-cced76b7df3f43b2bf5fafa041e0f6f8 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T21:16:02Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-cced76b7df3f43b2bf5fafa041e0f6f82023-11-19T16:26:06ZengMDPI AGFermentation2311-56372023-09-0191088110.3390/fermentation9100881Deeper Insights into the Effect of Humic Acid on Kitchen Waste Anaerobic Digestion: Enzyme Activities, Microbial Community Dynamics, and Key Metabolic PathwaysLin Lyu0Yanzeng Li1Shenghua Zhang2Zhou Chen3College of Harbour and Coastal Engineering, Jimei University, Xiamen 361021, ChinaInstitute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, ChinaCollege of Harbour and Coastal Engineering, Jimei University, Xiamen 361021, ChinaInstitute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, ChinaAnaerobic digestion (AD) represents one of the most eco-friendly approaches for recovering the energy from kitchen waste (KW). This study investigated the impact of humic acid (HA) on AD of KW. Batch experiments were conducted using KW as the substrate, with varying HA dosages. The results revealed that an increase in HA dosage led to an inhibition of methanogenic efficiency by 29.51% and a delayed start-up of AD. The HA exhibited dual effects on enzyme activities during AD, inhibiting hydrolytic enzymes while stimulating acidogenic enzymes. This unique interaction ultimately resulted in a significant 12.32% increase in volatile fatty acid production. Furthermore, HA induced the generation of reactive oxygen species and had a discernible impact on the activity of the electron transfer system. A bioinformatics analysis highlighted that HA promoted the abundance of microorganisms with mediated interspecies electron transfer ability, including DMER64 and Methanosaeta, as well as the Firmicutes phylum, while significantly reducing the abundance of Methanobacterium. Moreover, the KEGG pathway analysis revealed a decrease in hydrolysis and methanogenesis-related genes and an increase in acidogenesis-related genes.https://www.mdpi.com/2311-5637/9/10/881kitchen wasteanaerobic digestionhumic acidenzyme activitymetabolic pathways |
| spellingShingle | Lin Lyu Yanzeng Li Shenghua Zhang Zhou Chen Deeper Insights into the Effect of Humic Acid on Kitchen Waste Anaerobic Digestion: Enzyme Activities, Microbial Community Dynamics, and Key Metabolic Pathways Fermentation kitchen waste anaerobic digestion humic acid enzyme activity metabolic pathways |
| title | Deeper Insights into the Effect of Humic Acid on Kitchen Waste Anaerobic Digestion: Enzyme Activities, Microbial Community Dynamics, and Key Metabolic Pathways |
| title_full | Deeper Insights into the Effect of Humic Acid on Kitchen Waste Anaerobic Digestion: Enzyme Activities, Microbial Community Dynamics, and Key Metabolic Pathways |
| title_fullStr | Deeper Insights into the Effect of Humic Acid on Kitchen Waste Anaerobic Digestion: Enzyme Activities, Microbial Community Dynamics, and Key Metabolic Pathways |
| title_full_unstemmed | Deeper Insights into the Effect of Humic Acid on Kitchen Waste Anaerobic Digestion: Enzyme Activities, Microbial Community Dynamics, and Key Metabolic Pathways |
| title_short | Deeper Insights into the Effect of Humic Acid on Kitchen Waste Anaerobic Digestion: Enzyme Activities, Microbial Community Dynamics, and Key Metabolic Pathways |
| title_sort | deeper insights into the effect of humic acid on kitchen waste anaerobic digestion enzyme activities microbial community dynamics and key metabolic pathways |
| topic | kitchen waste anaerobic digestion humic acid enzyme activity metabolic pathways |
| url | https://www.mdpi.com/2311-5637/9/10/881 |
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