Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger
Abstract Background Aspergillus niger ATCC 20611 is an industrially important fructooligosaccharides (FOS) producer since it produces the β-fructofuranosidase with superior transglycosylation activity, which is responsible for the conversion of sucrose to FOS accompanied by the by-product (glucose)...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-03-01
|
| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-024-02353-w |
| _version_ | 1797266592050970624 |
|---|---|
| author | Xiufen Wan Lu Wang Jingjing Chang Jing Zhang Zhiyun Zhang Kewen Li Guilian Sun Caixia Liu Yaohua Zhong |
| author_facet | Xiufen Wan Lu Wang Jingjing Chang Jing Zhang Zhiyun Zhang Kewen Li Guilian Sun Caixia Liu Yaohua Zhong |
| author_sort | Xiufen Wan |
| collection | DOAJ |
| description | Abstract Background Aspergillus niger ATCC 20611 is an industrially important fructooligosaccharides (FOS) producer since it produces the β-fructofuranosidase with superior transglycosylation activity, which is responsible for the conversion of sucrose to FOS accompanied by the by-product (glucose) generation. This study aims to consume glucose to enhance the content of FOS by heterologously expressing glucose oxidase and peroxidase in engineered A. niger. Results Glucose oxidase was successfully expressed and co-localized with β-fructofuranosidase in mycelia. These mycelia were applied to synthesis of FOS, which possessed an increased purity of 60.63% from 52.07%. Furthermore, peroxidase was expressed in A. niger and reached 7.70 U/g, which could remove the potential inhibitor of glucose oxidase to facilitate the FOS synthesis. Finally, the glucose oxidase-expressing strain and the peroxidase-expressing strain were jointly used to synthesize FOS, which content achieved 71.00%. Conclusions This strategy allows for obtaining high-content FOS by the multiple enzymes expressed in the industrial fungus, avoiding additional purification processes used in the production of oligosaccharides. This study not only facilitated the high-purity FOS synthesis, but also demonstrated the potential of A. niger ATCC 20611 as an enzyme-producing cell factory. |
| first_indexed | 2024-04-25T01:03:08Z |
| format | Article |
| id | doaj.art-ccf0faabd65b4933bbdc1cacfe9cc26b |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-25T01:03:08Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-ccf0faabd65b4933bbdc1cacfe9cc26b2024-03-10T12:24:50ZengBMCMicrobial Cell Factories1475-28592024-03-0123111310.1186/s12934-024-02353-wEffective synthesis of high-content fructooligosaccharides in engineered Aspergillus nigerXiufen Wan0Lu Wang1Jingjing Chang2Jing Zhang3Zhiyun Zhang4Kewen Li5Guilian Sun6Caixia Liu7Yaohua Zhong8State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong UniversityShandong Academy of Pharmaceutical SciencesBaolingbao Biology Co., LtdBaolingbao Biology Co., LtdShandong Academy of Pharmaceutical SciencesState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong UniversityAbstract Background Aspergillus niger ATCC 20611 is an industrially important fructooligosaccharides (FOS) producer since it produces the β-fructofuranosidase with superior transglycosylation activity, which is responsible for the conversion of sucrose to FOS accompanied by the by-product (glucose) generation. This study aims to consume glucose to enhance the content of FOS by heterologously expressing glucose oxidase and peroxidase in engineered A. niger. Results Glucose oxidase was successfully expressed and co-localized with β-fructofuranosidase in mycelia. These mycelia were applied to synthesis of FOS, which possessed an increased purity of 60.63% from 52.07%. Furthermore, peroxidase was expressed in A. niger and reached 7.70 U/g, which could remove the potential inhibitor of glucose oxidase to facilitate the FOS synthesis. Finally, the glucose oxidase-expressing strain and the peroxidase-expressing strain were jointly used to synthesize FOS, which content achieved 71.00%. Conclusions This strategy allows for obtaining high-content FOS by the multiple enzymes expressed in the industrial fungus, avoiding additional purification processes used in the production of oligosaccharides. This study not only facilitated the high-purity FOS synthesis, but also demonstrated the potential of A. niger ATCC 20611 as an enzyme-producing cell factory.https://doi.org/10.1186/s12934-024-02353-wFructooligosaccharidesAspergillus nigerHeterologous expressionβ-fructofuranosidaseGlucose oxidasePeroxidase |
| spellingShingle | Xiufen Wan Lu Wang Jingjing Chang Jing Zhang Zhiyun Zhang Kewen Li Guilian Sun Caixia Liu Yaohua Zhong Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger Microbial Cell Factories Fructooligosaccharides Aspergillus niger Heterologous expression β-fructofuranosidase Glucose oxidase Peroxidase |
| title | Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger |
| title_full | Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger |
| title_fullStr | Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger |
| title_full_unstemmed | Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger |
| title_short | Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger |
| title_sort | effective synthesis of high content fructooligosaccharides in engineered aspergillus niger |
| topic | Fructooligosaccharides Aspergillus niger Heterologous expression β-fructofuranosidase Glucose oxidase Peroxidase |
| url | https://doi.org/10.1186/s12934-024-02353-w |
| work_keys_str_mv | AT xiufenwan effectivesynthesisofhighcontentfructooligosaccharidesinengineeredaspergillusniger AT luwang effectivesynthesisofhighcontentfructooligosaccharidesinengineeredaspergillusniger AT jingjingchang effectivesynthesisofhighcontentfructooligosaccharidesinengineeredaspergillusniger AT jingzhang effectivesynthesisofhighcontentfructooligosaccharidesinengineeredaspergillusniger AT zhiyunzhang effectivesynthesisofhighcontentfructooligosaccharidesinengineeredaspergillusniger AT kewenli effectivesynthesisofhighcontentfructooligosaccharidesinengineeredaspergillusniger AT guiliansun effectivesynthesisofhighcontentfructooligosaccharidesinengineeredaspergillusniger AT caixialiu effectivesynthesisofhighcontentfructooligosaccharidesinengineeredaspergillusniger AT yaohuazhong effectivesynthesisofhighcontentfructooligosaccharidesinengineeredaspergillusniger |