Spatially controlled construction of assembloids using bioprinting
Abstract The biofabrication of three-dimensional (3D) tissues that recapitulate organ-specific architecture and function would benefit from temporal and spatial control of cell-cell interactions. Bioprinting, while potentially capable of achieving such control, is poorly suited to organoids with con...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-40006-5 |
| _version_ | 1797774111665029120 |
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| author | Julien G. Roth Lucia G. Brunel Michelle S. Huang Yueming Liu Betty Cai Sauradeep Sinha Fan Yang Sergiu P. Pașca Sungchul Shin Sarah C. Heilshorn |
| author_facet | Julien G. Roth Lucia G. Brunel Michelle S. Huang Yueming Liu Betty Cai Sauradeep Sinha Fan Yang Sergiu P. Pașca Sungchul Shin Sarah C. Heilshorn |
| author_sort | Julien G. Roth |
| collection | DOAJ |
| description | Abstract The biofabrication of three-dimensional (3D) tissues that recapitulate organ-specific architecture and function would benefit from temporal and spatial control of cell-cell interactions. Bioprinting, while potentially capable of achieving such control, is poorly suited to organoids with conserved cytoarchitectures that are susceptible to plastic deformation. Here, we develop a platform, termed Spatially Patterned Organoid Transfer (SPOT), consisting of an iron-oxide nanoparticle laden hydrogel and magnetized 3D printer to enable the controlled lifting, transport, and deposition of organoids. We identify cellulose nanofibers as both an ideal biomaterial for encasing organoids with magnetic nanoparticles and a shear-thinning, self-healing support hydrogel for maintaining the spatial positioning of organoids to facilitate the generation of assembloids. We leverage SPOT to create precisely arranged assembloids composed of human pluripotent stem cell-derived neural organoids and patient-derived glioma organoids. In doing so, we demonstrate the potential for the SPOT platform to construct assembloids which recapitulate key developmental processes and disease etiologies. |
| first_indexed | 2024-03-12T22:16:16Z |
| format | Article |
| id | doaj.art-ccf6740b904b407fbc10859b9fe7c252 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-12T22:16:16Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-ccf6740b904b407fbc10859b9fe7c2522023-07-23T11:18:47ZengNature PortfolioNature Communications2041-17232023-07-0114111410.1038/s41467-023-40006-5Spatially controlled construction of assembloids using bioprintingJulien G. Roth0Lucia G. Brunel1Michelle S. Huang2Yueming Liu3Betty Cai4Sauradeep Sinha5Fan Yang6Sergiu P. Pașca7Sungchul Shin8Sarah C. Heilshorn9Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of MedicineDepartment of Chemical Engineering, Stanford UniversityDepartment of Chemical Engineering, Stanford UniversityDepartment of Materials Science and Engineering, Stanford UniversityDepartment of Materials Science and Engineering, Stanford UniversityDepartment of Bioengineering, Stanford UniversityDepartment of Bioengineering, Stanford UniversityStanford Brain Organogenesis, Wu Tsai Neurosciences Institute & Bio-X, Stanford UniversityDepartment of Materials Science and Engineering, Stanford UniversityStanford Brain Organogenesis, Wu Tsai Neurosciences Institute & Bio-X, Stanford UniversityAbstract The biofabrication of three-dimensional (3D) tissues that recapitulate organ-specific architecture and function would benefit from temporal and spatial control of cell-cell interactions. Bioprinting, while potentially capable of achieving such control, is poorly suited to organoids with conserved cytoarchitectures that are susceptible to plastic deformation. Here, we develop a platform, termed Spatially Patterned Organoid Transfer (SPOT), consisting of an iron-oxide nanoparticle laden hydrogel and magnetized 3D printer to enable the controlled lifting, transport, and deposition of organoids. We identify cellulose nanofibers as both an ideal biomaterial for encasing organoids with magnetic nanoparticles and a shear-thinning, self-healing support hydrogel for maintaining the spatial positioning of organoids to facilitate the generation of assembloids. We leverage SPOT to create precisely arranged assembloids composed of human pluripotent stem cell-derived neural organoids and patient-derived glioma organoids. In doing so, we demonstrate the potential for the SPOT platform to construct assembloids which recapitulate key developmental processes and disease etiologies.https://doi.org/10.1038/s41467-023-40006-5 |
| spellingShingle | Julien G. Roth Lucia G. Brunel Michelle S. Huang Yueming Liu Betty Cai Sauradeep Sinha Fan Yang Sergiu P. Pașca Sungchul Shin Sarah C. Heilshorn Spatially controlled construction of assembloids using bioprinting Nature Communications |
| title | Spatially controlled construction of assembloids using bioprinting |
| title_full | Spatially controlled construction of assembloids using bioprinting |
| title_fullStr | Spatially controlled construction of assembloids using bioprinting |
| title_full_unstemmed | Spatially controlled construction of assembloids using bioprinting |
| title_short | Spatially controlled construction of assembloids using bioprinting |
| title_sort | spatially controlled construction of assembloids using bioprinting |
| url | https://doi.org/10.1038/s41467-023-40006-5 |
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