DNA Barcoding unveils cryptic lineages of Hoplias malabaricus from Northeastern Brazil
Abstract The trahira or wolf fish - Hoplias malabaricus- is a valid species, although recent cytogenetic and molecular studies have indicated the existence of a species complex. In this context, the present study analyzed the mitochondrial COI marker to determine the levels of genetic diversity of s...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2020-10-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842021000400917&tlng=en |
| _version_ | 1819005252597186560 |
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| author | W. M. M. Pires M. C. Barros E. C. Fraga |
| author_facet | W. M. M. Pires M. C. Barros E. C. Fraga |
| author_sort | W. M. M. Pires |
| collection | DOAJ |
| description | Abstract The trahira or wolf fish - Hoplias malabaricus- is a valid species, although recent cytogenetic and molecular studies have indicated the existence of a species complex. In this context, the present study analyzed the mitochondrial COI marker to determine the levels of genetic diversity of specimens from the Brazilian state of Maranhão, and verify the occurrence of distinct lineages within the study area. Samples were collected from the basins of the Turiaçu, Pindaré, Mearim, Itapecuru, and Parnaíba rivers. A 630-bp fragment was obtained from 211 specimens, with 484 conserved and 108 variable sites, and 60 haplotypes (Hd = 0,947; π = 0,033). The phylogenetic analyses indicated the existence of three distinct lineages of H. malabaricus from Maranhão. Genetic distances of 1.5-8.2% were found between all the populations analyzed, while the variation between haplogroups ranged from 2.1% to 7.7%. The AMOVA indicated that most of the molecular variation was found among groups, with high FST values. The high levels of genetic variability found in the present study are supported by the available cytogenetic data. These findings reinforce the need for the development of effective programs of conservation and management independently for each river basin, in order to preserve the genetic variability found in this taxon. |
| first_indexed | 2024-12-20T23:49:50Z |
| format | Article |
| id | doaj.art-ccf84d3be2d34e7ab74703d305489461 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-20T23:49:50Z |
| publishDate | 2020-10-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-ccf84d3be2d34e7ab74703d3054894612022-12-21T19:22:52ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752020-10-0181491792710.1590/1519-6984.231598DNA Barcoding unveils cryptic lineages of Hoplias malabaricus from Northeastern BrazilW. M. M. Pireshttps://orcid.org/0000-0003-0893-736XM. C. Barroshttps://orcid.org/0000-0001-9055-1911E. C. Fragahttps://orcid.org/0000-0001-8062-0338Abstract The trahira or wolf fish - Hoplias malabaricus- is a valid species, although recent cytogenetic and molecular studies have indicated the existence of a species complex. In this context, the present study analyzed the mitochondrial COI marker to determine the levels of genetic diversity of specimens from the Brazilian state of Maranhão, and verify the occurrence of distinct lineages within the study area. Samples were collected from the basins of the Turiaçu, Pindaré, Mearim, Itapecuru, and Parnaíba rivers. A 630-bp fragment was obtained from 211 specimens, with 484 conserved and 108 variable sites, and 60 haplotypes (Hd = 0,947; π = 0,033). The phylogenetic analyses indicated the existence of three distinct lineages of H. malabaricus from Maranhão. Genetic distances of 1.5-8.2% were found between all the populations analyzed, while the variation between haplogroups ranged from 2.1% to 7.7%. The AMOVA indicated that most of the molecular variation was found among groups, with high FST values. The high levels of genetic variability found in the present study are supported by the available cytogenetic data. These findings reinforce the need for the development of effective programs of conservation and management independently for each river basin, in order to preserve the genetic variability found in this taxon.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842021000400917&tlng=encytotypeCOIspecies complexcryptic speciationtrahira |
| spellingShingle | W. M. M. Pires M. C. Barros E. C. Fraga DNA Barcoding unveils cryptic lineages of Hoplias malabaricus from Northeastern Brazil Brazilian Journal of Biology cytotype COI species complex cryptic speciation trahira |
| title | DNA Barcoding unveils cryptic lineages of Hoplias malabaricus from Northeastern Brazil |
| title_full | DNA Barcoding unveils cryptic lineages of Hoplias malabaricus from Northeastern Brazil |
| title_fullStr | DNA Barcoding unveils cryptic lineages of Hoplias malabaricus from Northeastern Brazil |
| title_full_unstemmed | DNA Barcoding unveils cryptic lineages of Hoplias malabaricus from Northeastern Brazil |
| title_short | DNA Barcoding unveils cryptic lineages of Hoplias malabaricus from Northeastern Brazil |
| title_sort | dna barcoding unveils cryptic lineages of hoplias malabaricus from northeastern brazil |
| topic | cytotype COI species complex cryptic speciation trahira |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842021000400917&tlng=en |
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