Calcium dynamics of cortical astrocytic networks in vivo.
Large and long-lasting cytosolic calcium surges in astrocytes have been described in cultured cells and acute slice preparations. The mechanisms that give rise to these calcium events have been extensively studied in vitro. However, their existence and functions in the intact brain are unknown. We h...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2004-04-01
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| Series: | PLoS Biology |
| Online Access: | http://europepmc.org/articles/PMC387267?pdf=render |
| _version_ | 1819149218348007424 |
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| author | Hajime Hirase Lifen Qian Peter Barthó György Buzsáki |
| author_facet | Hajime Hirase Lifen Qian Peter Barthó György Buzsáki |
| author_sort | Hajime Hirase |
| collection | DOAJ |
| description | Large and long-lasting cytosolic calcium surges in astrocytes have been described in cultured cells and acute slice preparations. The mechanisms that give rise to these calcium events have been extensively studied in vitro. However, their existence and functions in the intact brain are unknown. We have topically applied Fluo-4 AM on the cerebral cortex of anesthetized rats, and imaged cytosolic calcium fluctuation in astrocyte populations of superficial cortical layers in vivo, using two-photon laser scanning microscopy. Spontaneous [Ca(2+)](i) events in individual astrocytes were similar to those observed in vitro. Coordination of [Ca(2+)](i) events among astrocytes was indicated by the broad cross-correlograms. Increased neuronal discharge was associated with increased astrocytic [Ca(2+)](i) activity in individual cells and a robust coordination of [Ca(2+)](i) signals in neighboring astrocytes. These findings indicate potential neuron-glia communication in the intact brain. |
| first_indexed | 2024-12-22T13:58:07Z |
| format | Article |
| id | doaj.art-ccfaff9bb7da47bc80bc7276620f3d5d |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-22T13:58:07Z |
| publishDate | 2004-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-ccfaff9bb7da47bc80bc7276620f3d5d2022-12-21T18:23:29ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852004-04-0124E9610.1371/journal.pbio.0020096Calcium dynamics of cortical astrocytic networks in vivo.Hajime HiraseLifen QianPeter BarthóGyörgy BuzsákiLarge and long-lasting cytosolic calcium surges in astrocytes have been described in cultured cells and acute slice preparations. The mechanisms that give rise to these calcium events have been extensively studied in vitro. However, their existence and functions in the intact brain are unknown. We have topically applied Fluo-4 AM on the cerebral cortex of anesthetized rats, and imaged cytosolic calcium fluctuation in astrocyte populations of superficial cortical layers in vivo, using two-photon laser scanning microscopy. Spontaneous [Ca(2+)](i) events in individual astrocytes were similar to those observed in vitro. Coordination of [Ca(2+)](i) events among astrocytes was indicated by the broad cross-correlograms. Increased neuronal discharge was associated with increased astrocytic [Ca(2+)](i) activity in individual cells and a robust coordination of [Ca(2+)](i) signals in neighboring astrocytes. These findings indicate potential neuron-glia communication in the intact brain.http://europepmc.org/articles/PMC387267?pdf=render |
| spellingShingle | Hajime Hirase Lifen Qian Peter Barthó György Buzsáki Calcium dynamics of cortical astrocytic networks in vivo. PLoS Biology |
| title | Calcium dynamics of cortical astrocytic networks in vivo. |
| title_full | Calcium dynamics of cortical astrocytic networks in vivo. |
| title_fullStr | Calcium dynamics of cortical astrocytic networks in vivo. |
| title_full_unstemmed | Calcium dynamics of cortical astrocytic networks in vivo. |
| title_short | Calcium dynamics of cortical astrocytic networks in vivo. |
| title_sort | calcium dynamics of cortical astrocytic networks in vivo |
| url | http://europepmc.org/articles/PMC387267?pdf=render |
| work_keys_str_mv | AT hajimehirase calciumdynamicsofcorticalastrocyticnetworksinvivo AT lifenqian calciumdynamicsofcorticalastrocyticnetworksinvivo AT peterbartho calciumdynamicsofcorticalastrocyticnetworksinvivo AT gyorgybuzsaki calciumdynamicsofcorticalastrocyticnetworksinvivo |